biobox/target/executable/lofreq/lofreq_call/lofreq_call

#!/usr/bin/env bash

# lofreq_call main
# 
# This wrapper script is auto-generated by viash 0.9.0-RC7 and is thus a
# derivative work thereof. This software comes with ABSOLUTELY NO WARRANTY from
# Data Intuitive.
# 
# The component may contain files which fall under a different license. The
# authors of this component should specify the license in the header of such
# files, or include a separate license file detailing the licenses of all included
# files.
# 
# Component authors:
#  * Kai Waldrant (author, maintainer)

set -e

if [ -z "$VIASH_TEMP" ]; then
  VIASH_TEMP=${VIASH_TEMP:-$VIASH_TMPDIR}
  VIASH_TEMP=${VIASH_TEMP:-$VIASH_TEMPDIR}
  VIASH_TEMP=${VIASH_TEMP:-$VIASH_TMP}
  VIASH_TEMP=${VIASH_TEMP:-$TMPDIR}
  VIASH_TEMP=${VIASH_TEMP:-$TMP}
  VIASH_TEMP=${VIASH_TEMP:-$TEMPDIR}
  VIASH_TEMP=${VIASH_TEMP:-$TEMP}
  VIASH_TEMP=${VIASH_TEMP:-/tmp}
fi

# define helper functions
# ViashQuote: put quotes around non flag values
# $1     : unquoted string
# return : possibly quoted string
# examples:
#   ViashQuote --foo      # returns --foo
#   ViashQuote bar        # returns 'bar'
#   Viashquote --foo=bar  # returns --foo='bar'
function ViashQuote {
  if [[ "$1" =~ ^-+[a-zA-Z0-9_\-]+=.+$ ]]; then
    echo "$1" | sed "s#=\(.*\)#='\1'#"
  elif [[ "$1" =~ ^-+[a-zA-Z0-9_\-]+$ ]]; then
    echo "$1"
  else
    echo "'$1'"
  fi
}
# ViashRemoveFlags: Remove leading flag
# $1     : string with a possible leading flag
# return : string without possible leading flag
# examples:
#   ViashRemoveFlags --foo=bar  # returns bar
function ViashRemoveFlags {
  echo "$1" | sed 's/^--*[a-zA-Z0-9_\-]*=//'
}
# ViashSourceDir: return the path of a bash file, following symlinks
# usage   : ViashSourceDir ${BASH_SOURCE[0]}
# $1      : Should always be set to ${BASH_SOURCE[0]}
# returns : The absolute path of the bash file
function ViashSourceDir {
  local source="$1"
  while [ -h "$source" ]; do
    local dir="$( cd -P "$( dirname "$source" )" >/dev/null 2>&1 && pwd )"
    source="$(readlink "$source")"
    [[ $source != /* ]] && source="$dir/$source"
  done
  cd -P "$( dirname "$source" )" >/dev/null 2>&1 && pwd
}
# ViashFindTargetDir: return the path of the '.build.yaml' file, following symlinks
# usage   : ViashFindTargetDir 'ScriptPath'
# $1      : The location from where to start the upward search
# returns : The absolute path of the '.build.yaml' file
function ViashFindTargetDir {
  local source="$1"
  while [[ "$source" != "" && ! -e "$source/.build.yaml" ]]; do
    source=${source%/*}
  done
  echo $source
}
# see https://en.wikipedia.org/wiki/Syslog#Severity_level
VIASH_LOGCODE_EMERGENCY=0
VIASH_LOGCODE_ALERT=1
VIASH_LOGCODE_CRITICAL=2
VIASH_LOGCODE_ERROR=3
VIASH_LOGCODE_WARNING=4
VIASH_LOGCODE_NOTICE=5
VIASH_LOGCODE_INFO=6
VIASH_LOGCODE_DEBUG=7
VIASH_VERBOSITY=$VIASH_LOGCODE_NOTICE

# ViashLog: Log events depending on the verbosity level
# usage: ViashLog 1 alert Oh no something went wrong!
# $1: required verbosity level
# $2: display tag
# $3+: messages to display
# stdout: Your input, prepended by '[$2] '.
function ViashLog {
  local required_level="$1"
  local display_tag="$2"
  shift 2
  if [ $VIASH_VERBOSITY -ge $required_level ]; then
    >&2 echo "[$display_tag]" "$@"
  fi
}

# ViashEmergency: log events when the system is unstable
# usage: ViashEmergency Oh no something went wrong.
# stdout: Your input, prepended by '[emergency] '.
function ViashEmergency {
  ViashLog $VIASH_LOGCODE_EMERGENCY emergency "$@"
}

# ViashAlert: log events when actions must be taken immediately (e.g. corrupted system database)
# usage: ViashAlert Oh no something went wrong.
# stdout: Your input, prepended by '[alert] '.
function ViashAlert {
  ViashLog $VIASH_LOGCODE_ALERT alert "$@"
}

# ViashCritical: log events when a critical condition occurs
# usage: ViashCritical Oh no something went wrong.
# stdout: Your input, prepended by '[critical] '.
function ViashCritical {
  ViashLog $VIASH_LOGCODE_CRITICAL critical "$@"
}

# ViashError: log events when an error condition occurs
# usage: ViashError Oh no something went wrong.
# stdout: Your input, prepended by '[error] '.
function ViashError {
  ViashLog $VIASH_LOGCODE_ERROR error "$@"
}

# ViashWarning: log potentially abnormal events
# usage: ViashWarning Something may have gone wrong.
# stdout: Your input, prepended by '[warning] '.
function ViashWarning {
  ViashLog $VIASH_LOGCODE_WARNING warning "$@"
}

# ViashNotice: log significant but normal events
# usage: ViashNotice This just happened.
# stdout: Your input, prepended by '[notice] '.
function ViashNotice {
  ViashLog $VIASH_LOGCODE_NOTICE notice "$@"
}

# ViashInfo: log normal events
# usage: ViashInfo This just happened.
# stdout: Your input, prepended by '[info] '.
function ViashInfo {
  ViashLog $VIASH_LOGCODE_INFO info "$@"
}

# ViashDebug: log all events, for debugging purposes
# usage: ViashDebug This just happened.
# stdout: Your input, prepended by '[debug] '.
function ViashDebug {
  ViashLog $VIASH_LOGCODE_DEBUG debug "$@"
}

# find source folder of this component
VIASH_META_RESOURCES_DIR=`ViashSourceDir ${BASH_SOURCE[0]}`

# find the root of the built components & dependencies
VIASH_TARGET_DIR=`ViashFindTargetDir $VIASH_META_RESOURCES_DIR`

# define meta fields
VIASH_META_NAME="lofreq_call"
VIASH_META_FUNCTIONALITY_NAME="lofreq_call"
VIASH_META_EXECUTABLE="$VIASH_META_RESOURCES_DIR/$VIASH_META_NAME"
VIASH_META_CONFIG="$VIASH_META_RESOURCES_DIR/.config.vsh.yaml"
VIASH_META_TEMP_DIR="$VIASH_TEMP"


# ViashHelp: Display helpful explanation about this executable
function ViashHelp {
  echo "lofreq_call main"
  echo ""
  echo "Call variants from a BAM file."
  echo ""
  echo "LoFreq* (i.e. LoFreq version 2) is a fast and sensitive variant-caller for"
  echo "inferring SNVs and indels from next-generation sequencing data. It makes full"
  echo "use of base-call qualities and other sources of errors inherent in sequencing"
  echo "(e.g. mapping or base/indel alignment uncertainty), which are usually ignored by"
  echo "other methods or only used for filtering."
  echo ""
  echo "LoFreq* can run on almost any type of aligned sequencing data (e.g. Illumina,"
  echo "IonTorrent or Pacbio) since no machine- or sequencing-technology dependent"
  echo "thresholds are used. It automatically adapts to changes in coverage and"
  echo "sequencing quality and can therefore be applied to a variety of data-sets e.g."
  echo "viral/quasispecies, bacterial, metagenomics or somatic data."
  echo ""
  echo "LoFreq* is very sensitive; most notably, it is able to predict variants below"
  echo "the average base-call quality (i.e. sequencing error rate). Each variant call is"
  echo "assigned a p-value which allows for rigorous false positive control. Even though"
  echo "it uses no approximations or heuristics, it is very efficient due to several"
  echo "runtime optimizations and also provides a (pseudo-)parallel implementation."
  echo "LoFreq* is generic and fast enough to be applied to high-coverage data and large"
  echo "genomes. On a single processor it takes a minute to analyze Dengue genome"
  echo "sequencing data with nearly 4000X coverage, roughly one hour to call SNVs on a"
  echo "600X coverage E.coli genome and also roughly an hour to run on a 100X coverage"
  echo "human exome dataset."
  echo ""
  echo "Inputs:"
  echo "    --input"
  echo "        type: file, required parameter, file must exist"
  echo "        example: normal.bam"
  echo "        Input BAM file."
  echo ""
  echo "    --input_bai"
  echo "        type: file, required parameter, file must exist"
  echo "        example: normal.bai"
  echo "        Index file for the input BAM file."
  echo ""
  echo "    -f, --ref"
  echo "        type: file, required parameter, file must exist"
  echo "        example: reference.fasta"
  echo "        Indexed reference fasta file (gzip supported). Default: none."
  echo ""
  echo "Outputs:"
  echo "    -o, --out"
  echo "        type: file, required parameter, output, file must exist"
  echo "        example: output.vcf"
  echo "        Vcf output file. Default: stdout."
  echo ""
  echo "Arguments:"
  echo "    -r, --region"
  echo "        type: string"
  echo "        example: chr1:1000-2000"
  echo "        Limit calls to this region (chrom:start-end). Default: none."
  echo ""
  echo "    -l, --bed"
  echo "        type: file, file must exist"
  echo "        example: regions.bed"
  echo "        List of positions (chr pos) or regions (BED). Default: none."
  echo ""
  echo "    -q, --min_bq"
  echo "        type: integer"
  echo "        example: 6"
  echo "        Skip any base with baseQ smaller than INT. Default: 6."
  echo ""
  echo "    -Q, --min_alt_bq"
  echo "        type: integer"
  echo "        example: 6"
  echo "        Skip alternate bases with baseQ smaller than INT. Default: 6."
  echo ""
  echo "    -R, --def_alt_bq"
  echo "        type: integer"
  echo "        example: 0"
  echo "        Overwrite baseQs of alternate bases (that passed bq filter) with this"
  echo "        value (-1: use median ref-bq; 0: keep). Default: 0."
  echo ""
  echo "    -j, --min_jq"
  echo "        type: integer"
  echo "        example: 0"
  echo "        Skip any base with joinedQ smaller than INT. Default: 0."
  echo ""
  echo "    -J, --min_alt_jq"
  echo "        type: integer"
  echo "        example: 0"
  echo "        Skip alternate bases with joinedQ smaller than INT. Default: 0."
  echo ""
  echo "    -K, --def_alt_jq"
  echo "        type: integer"
  echo "        example: 0"
  echo "        Overwrite joinedQs of alternate bases (that passed jq filter) with this"
  echo "        value (-1: use median ref-bq; 0: keep). Default: 0."
  echo ""
  echo "    -B, --no_baq"
  echo "        type: boolean_true"
  echo "        Disable use of base-alignment quality (BAQ)."
  echo ""
  echo "    -A, --no_idaq"
  echo "        type: boolean_true"
  echo "        Don't use IDAQ values (NOT recommended under ANY circumstances other"
  echo "        than debugging)."
  echo ""
  echo "    -D, --del_baq"
  echo "        type: boolean_true"
  echo "        Delete pre-existing BAQ values, i.e. compute even if already present in"
  echo "        BAM."
  echo ""
  echo "    -e, --no_ext_baq"
  echo "        type: boolean_true"
  echo "        Use 'normal' BAQ (samtools default) instead of extended BAQ (both"
  echo "        computed on the fly if not already present in lb tag)."
  echo ""
  echo "    -m, --min_mq"
  echo "        type: integer"
  echo "        example: 0"
  echo "        Skip reads with mapping quality smaller than INT. Default: 0."
  echo ""
  echo "    -M, --max_mq"
  echo "        type: integer"
  echo "        example: 255"
  echo "        Cap mapping quality at INT. Default: 255."
  echo ""
  echo "    -N, --no_mq"
  echo "        type: boolean_true"
  echo "        Don't merge mapping quality in LoFreq's model."
  echo ""
  echo "    --call_indels"
  echo "        type: boolean_true"
  echo "        Enable indel calls (note: preprocess your file to include indel"
  echo "        alignment qualities!)."
  echo ""
  echo "    --only_indels"
  echo "        type: boolean_true"
  echo "        Only call indels; no SNVs."
  echo ""
  echo "    -s, --src_qual"
  echo "        type: boolean_true"
  echo "        Enable computation of source quality."
  echo ""
  echo "    -S, --ign_vcf"
  echo "        type: file, file must exist"
  echo "        example: variants.vcf"
  echo "        Ignore variants in this vcf file for source quality computation."
  echo "        Multiple files can be given separated by commas."
  echo ""
  echo "    -T, --def_nm_q"
  echo "        type: integer"
  echo "        example: -1"
  echo "        If >= 0, then replace non-match base qualities with this default value."
  echo "        Default: -1."
  echo ""
  echo "    -a, --sig"
  echo "        type: double"
  echo "        example: 0.01"
  echo "        P-Value cutoff / significance level. Default: 0.010000."
  echo ""
  echo "    -b, --bonf"
  echo "        type: string"
  echo "        example: dynamic"
  echo "        Bonferroni factor. 'dynamic' (increase per actually performed test) or"
  echo "        INT. Default: Dynamic."
  echo ""
  echo "    -C, --min_cov"
  echo "        type: integer"
  echo "        example: 1"
  echo "        Test only positions having at least this coverage. Default: 1."
  echo "        (note: without --no-default-filter default filters (incl. coverage) kick"
  echo "        in after predictions are done)."
  echo ""
  echo "    -d, --max_depth"
  echo "        type: integer"
  echo "        example: 1000000"
  echo "        Cap coverage at this depth. Default: 1000000."
  echo ""
  echo "    --illumina_13"
  echo "        type: boolean_true"
  echo "        Assume the quality is Illumina-1.3-1.7/ASCII+64 encoded."
  echo ""
  echo "    --use_orphan"
  echo "        type: boolean_true"
  echo "        Count anomalous read pairs (i.e. where mate is not aligned properly)."
  echo ""
  echo "    --plp_summary_only"
  echo "        type: boolean_true"
  echo "        No variant calling. Just output pileup summary per column."
  echo ""
  echo "    --no_default_filter"
  echo "        type: boolean_true"
  echo "        Don't run default 'lofreq filter' automatically after calling variants."
  echo ""
  echo "    --force_overwrite"
  echo "        type: boolean_true"
  echo "        Overwrite any existing output."
  echo ""
  echo "    --verbose"
  echo "        type: boolean_true"
  echo "        Be verbose."
  echo ""
  echo "    --debug"
  echo "        type: boolean_true"
  echo "        Enable debugging."
}

# initialise variables
VIASH_MODE='run'
VIASH_ENGINE_ID='docker'

######## Helper functions for setting up Docker images for viash ########
# expects: ViashDockerBuild

# ViashDockerInstallationCheck: check whether Docker is installed correctly
#
# examples:
#   ViashDockerInstallationCheck
function ViashDockerInstallationCheck {
  ViashDebug "Checking whether Docker is installed"
  if [ ! command -v docker &> /dev/null ]; then
    ViashCritical "Docker doesn't seem to be installed. See 'https://docs.docker.com/get-docker/' for instructions."
    exit 1
  fi

  ViashDebug "Checking whether the Docker daemon is running"
  local save=$-; set +e
  local docker_version=$(docker version --format '{{.Client.APIVersion}}' 2> /dev/null)
  local out=$?
  [[ $save =~ e ]] && set -e
  if [ $out -ne 0 ]; then
    ViashCritical "Docker daemon does not seem to be running. Try one of the following:"
    ViashCritical "- Try running 'dockerd' in the command line"
    ViashCritical "- See https://docs.docker.com/config/daemon/"
    exit 1
  fi
}

# ViashDockerRemoteTagCheck: check whether a Docker image is available 
# on a remote. Assumes `docker login` has been performed, if relevant.
#
# $1                  : image identifier with format `[registry/]image[:tag]`
# exit code $?        : whether or not the image was found
# examples:
#   ViashDockerRemoteTagCheck python:latest
#   echo $?                                     # returns '0'
#   ViashDockerRemoteTagCheck sdaizudceahifu
#   echo $?                                     # returns '1'
function ViashDockerRemoteTagCheck {
  docker manifest inspect $1 > /dev/null 2> /dev/null
}

# ViashDockerLocalTagCheck: check whether a Docker image is available locally
#
# $1                  : image identifier with format `[registry/]image[:tag]`
# exit code $?        : whether or not the image was found
# examples:
#   docker pull python:latest
#   ViashDockerLocalTagCheck python:latest
#   echo $?                                     # returns '0'
#   ViashDockerLocalTagCheck sdaizudceahifu
#   echo $?                                     # returns '1'
function ViashDockerLocalTagCheck {
  [ -n "$(docker images -q $1)" ]
}

# ViashDockerPull: pull a Docker image
#
# $1                  : image identifier with format `[registry/]image[:tag]`
# exit code $?        : whether or not the image was found
# examples:
#   ViashDockerPull python:latest
#   echo $?                                     # returns '0'
#   ViashDockerPull sdaizudceahifu
#   echo $?                                     # returns '1'
function ViashDockerPull {
  ViashNotice "Checking if Docker image is available at '$1'"
  if [ $VIASH_VERBOSITY -ge $VIASH_LOGCODE_INFO ]; then
    docker pull $1 && return 0 || return 1
  else
    local save=$-; set +e
    docker pull $1 2> /dev/null > /dev/null
    local out=$?
    [[ $save =~ e ]] && set -e
    if [ $out -ne 0 ]; then
      ViashWarning "Could not pull from '$1'. Docker image doesn't exist or is not accessible."
    fi
    return $out
  fi
}

# ViashDockerPush: push a Docker image
#
# $1                  : image identifier with format `[registry/]image[:tag]`
# exit code $?        : whether or not the image was found
# examples:
#   ViashDockerPush python:latest
#   echo $?                                     # returns '0'
#   ViashDockerPush sdaizudceahifu
#   echo $?                                     # returns '1'
function ViashDockerPush {
  ViashNotice "Pushing image to '$1'"
  local save=$-; set +e
  local out
  if [ $VIASH_VERBOSITY -ge $VIASH_LOGCODE_INFO ]; then
    docker push $1
    out=$?
  else
    docker push $1 2> /dev/null > /dev/null
    out=$?
  fi
  [[ $save =~ e ]] && set -e
  if [ $out -eq 0 ]; then
    ViashNotice "Container '$1' push succeeded."
  else
    ViashError "Container '$1' push errored. You might not be logged in or have the necessary permissions."
  fi
  return $out
}

# ViashDockerPullElseBuild: pull a Docker image, else build it
#
# $1                  : image identifier with format `[registry/]image[:tag]`
# ViashDockerBuild    : a Bash function which builds a docker image, takes image identifier as argument.
# examples:
#   ViashDockerPullElseBuild mynewcomponent
function ViashDockerPullElseBuild {
  local save=$-; set +e
  ViashDockerPull $1
  local out=$?
  [[ $save =~ e ]] && set -e
  if [ $out -ne 0 ]; then
    ViashDockerBuild $@
  fi
}

# ViashDockerSetup: create a Docker image, according to specified docker setup strategy
#
# $1          : image identifier with format `[registry/]image[:tag]`
# $2          : docker setup strategy, see DockerSetupStrategy.scala
# examples:
#   ViashDockerSetup mynewcomponent alwaysbuild
function ViashDockerSetup {
  local image_id="$1"
  local setup_strategy="$2"
  if [ "$setup_strategy" == "alwaysbuild" -o "$setup_strategy" == "build" -o "$setup_strategy" == "b" ]; then
    ViashDockerBuild $image_id --no-cache $(ViashDockerBuildArgs "$engine_id")
  elif [ "$setup_strategy" == "alwayspull" -o "$setup_strategy" == "pull" -o "$setup_strategy" == "p" ]; then
    ViashDockerPull $image_id
  elif [ "$setup_strategy" == "alwayspullelsebuild" -o "$setup_strategy" == "pullelsebuild" ]; then
    ViashDockerPullElseBuild $image_id --no-cache $(ViashDockerBuildArgs "$engine_id")
  elif [ "$setup_strategy" == "alwayspullelsecachedbuild" -o "$setup_strategy" == "pullelsecachedbuild" ]; then
    ViashDockerPullElseBuild $image_id $(ViashDockerBuildArgs "$engine_id")
  elif [ "$setup_strategy" == "alwayscachedbuild" -o "$setup_strategy" == "cachedbuild" -o "$setup_strategy" == "cb" ]; then
    ViashDockerBuild $image_id $(ViashDockerBuildArgs "$engine_id")
  elif [[ "$setup_strategy" =~ ^ifneedbe ]]; then
    local save=$-; set +e
    ViashDockerLocalTagCheck $image_id
    local outCheck=$?
    [[ $save =~ e ]] && set -e
    if [ $outCheck -eq 0 ]; then
      ViashInfo "Image $image_id already exists"
    elif [ "$setup_strategy" == "ifneedbebuild" ]; then
      ViashDockerBuild $image_id --no-cache $(ViashDockerBuildArgs "$engine_id")
    elif [ "$setup_strategy" == "ifneedbecachedbuild" ]; then
      ViashDockerBuild $image_id $(ViashDockerBuildArgs "$engine_id")
    elif [ "$setup_strategy" == "ifneedbepull" ]; then
      ViashDockerPull $image_id
    elif [ "$setup_strategy" == "ifneedbepullelsebuild" ]; then
      ViashDockerPullElseBuild $image_id --no-cache $(ViashDockerBuildArgs "$engine_id")
    elif [ "$setup_strategy" == "ifneedbepullelsecachedbuild" ]; then
      ViashDockerPullElseBuild $image_id $(ViashDockerBuildArgs "$engine_id")
    else
      ViashError "Unrecognised Docker strategy: $setup_strategy"
      exit 1
    fi
  elif [ "$setup_strategy" == "push" -o "$setup_strategy" == "forcepush" -o "$setup_strategy" == "alwayspush" ]; then
    ViashDockerPush "$image_id"
  elif [ "$setup_strategy" == "pushifnotpresent" -o "$setup_strategy" == "gentlepush" -o "$setup_strategy" == "maybepush" ]; then
    local save=$-; set +e
    ViashDockerRemoteTagCheck $image_id
    local outCheck=$?
    [[ $save =~ e ]] && set -e
    if [ $outCheck -eq 0 ]; then
      ViashNotice "Container '$image_id' exists, doing nothing."
    else
      ViashNotice "Container '$image_id' does not yet exist."
      ViashDockerPush "$image_id"
    fi
  elif [ "$setup_strategy" == "donothing" -o "$setup_strategy" == "meh" ]; then
    ViashNotice "Skipping setup."
  else
    ViashError "Unrecognised Docker strategy: $setup_strategy"
    exit 1
  fi
}

# ViashDockerCheckCommands: Check whether a docker container has the required commands
#
# $1                  : image identifier with format `[registry/]image[:tag]`
# $@                  : commands to verify being present
# examples:
#   ViashDockerCheckCommands bash:4.0 bash ps foo
function ViashDockerCheckCommands {
  local image_id="$1"
  shift 1
  local commands="$@"
  local save=$-; set +e
  local missing # mark 'missing' as local in advance, otherwise the exit code of the command will be missing and always be '0'
  missing=$(docker run --rm --entrypoint=sh "$image_id" -c "for command in $commands; do command -v \$command >/dev/null 2>&1; if [ \$? -ne 0 ]; then echo \$command; exit 1; fi; done")
  local outCheck=$?
  [[ $save =~ e ]] && set -e
  if [ $outCheck -ne 0 ]; then
  	ViashError "Docker container '$image_id' does not contain command '$missing'."
  	exit 1
  fi
}

# ViashDockerBuild: build a docker image
# $1                               : image identifier with format `[registry/]image[:tag]`
# $...                             : additional arguments to pass to docker build
# $VIASH_META_TEMP_DIR             : temporary directory to store dockerfile & optional resources in
# $VIASH_META_NAME                 : name of the component
# $VIASH_META_RESOURCES_DIR        : directory containing the resources
# $VIASH_VERBOSITY                 : verbosity level
# exit code $?                     : whether or not the image was built successfully
function ViashDockerBuild {
  local image_id="$1"
  shift 1

  # create temporary directory to store dockerfile & optional resources in
  local tmpdir=$(mktemp -d "$VIASH_META_TEMP_DIR/dockerbuild-$VIASH_META_NAME-XXXXXX")
  local dockerfile="$tmpdir/Dockerfile"
  function clean_up {
    rm -rf "$tmpdir"
  }
  trap clean_up EXIT

  # store dockerfile and resources
  ViashDockerfile "$VIASH_ENGINE_ID" > "$dockerfile"

  # generate the build command
  local docker_build_cmd="docker build -t '$image_id' $@ '$VIASH_META_RESOURCES_DIR' -f '$dockerfile'"

  # build the container
  ViashNotice "Building container '$image_id' with Dockerfile"
  ViashInfo "$docker_build_cmd"
  local save=$-; set +e
  if [ $VIASH_VERBOSITY -ge $VIASH_LOGCODE_INFO ]; then
    eval $docker_build_cmd
  else
    eval $docker_build_cmd &> "$tmpdir/docker_build.log"
  fi

  # check exit code
  local out=$?
  [[ $save =~ e ]] && set -e
  if [ $out -ne 0 ]; then
    ViashError "Error occurred while building container '$image_id'"
    if [ $VIASH_VERBOSITY -lt $VIASH_LOGCODE_INFO ]; then
      ViashError "Transcript: --------------------------------"
      cat "$tmpdir/docker_build.log"
      ViashError "End of transcript --------------------------"
    fi
    exit 1
  fi
}

######## End of helper functions for setting up Docker images for viash ########

# ViashDockerFile: print the dockerfile to stdout
# $1    : engine identifier
# return : dockerfile required to run this component
# examples:
#   ViashDockerFile
function ViashDockerfile {
  local engine_id="$1"

  if [[ "$engine_id" == "docker" ]]; then
    cat << 'VIASHDOCKER'
FROM quay.io/biocontainers/lofreq:2.1.5--py38h794fc9e_10
ENTRYPOINT []
RUN version=$(lofreq version | grep 'version' | sed 's/version: //') && \
echo "lofreq: $version" > /var/software_versions.txt

LABEL org.opencontainers.image.authors="Kai Waldrant"
LABEL org.opencontainers.image.description="Companion container for running component lofreq lofreq_call"
LABEL org.opencontainers.image.created="2024-09-09T06:53:25Z"
LABEL org.opencontainers.image.source="https://github.com/viash-hub/biobox"
LABEL org.opencontainers.image.revision="320d044fe45e565fbc9772640ebf6f39c5584b4a"
LABEL org.opencontainers.image.version="main"

VIASHDOCKER
  fi
}

# ViashDockerBuildArgs: return the arguments to pass to docker build
# $1    : engine identifier
# return : arguments to pass to docker build
function ViashDockerBuildArgs {
  local engine_id="$1"

  if [[ "$engine_id" == "docker" ]]; then
    echo ""
  fi
}

# ViashAbsolutePath: generate absolute path from relative path
# borrowed from https://stackoverflow.com/a/21951256
# $1     : relative filename
# return : absolute path
# examples:
#   ViashAbsolutePath some_file.txt   # returns /path/to/some_file.txt
#   ViashAbsolutePath /foo/bar/..     # returns /foo
function ViashAbsolutePath {
  local thePath
  local parr
  local outp
  local len
  if [[ ! "$1" =~ ^/ ]]; then
    thePath="$PWD/$1"
  else
    thePath="$1"
  fi
  echo "$thePath" | (
    IFS=/
    read -a parr
    declare -a outp
    for i in "${parr[@]}"; do
      case "$i" in
      ''|.) continue ;;
      ..)
        len=${#outp[@]}
        if ((len==0)); then
          continue
        else
          unset outp[$((len-1))]
        fi
        ;;
      *)
        len=${#outp[@]}
        outp[$len]="$i"
      ;;
      esac
    done
    echo /"${outp[*]}"
  )
}
# ViashDockerAutodetectMount: auto configuring docker mounts from parameters
# $1                             : The parameter value
# returns                        : New parameter
# $VIASH_DIRECTORY_MOUNTS        : Added another parameter to be passed to docker
# $VIASH_DOCKER_AUTOMOUNT_PREFIX : The prefix to be used for the automounts
# examples:
#   ViashDockerAutodetectMount /path/to/bar      # returns '/viash_automount/path/to/bar'
#   ViashDockerAutodetectMountArg /path/to/bar   # returns '--volume="/path/to:/viash_automount/path/to"'
function ViashDockerAutodetectMount {
  local abs_path=$(ViashAbsolutePath "$1")
  local mount_source
  local base_name
  if [ -d "$abs_path" ]; then
    mount_source="$abs_path"
    base_name=""
  else
    mount_source=`dirname "$abs_path"`
    base_name=`basename "$abs_path"`
  fi
  local mount_target="$VIASH_DOCKER_AUTOMOUNT_PREFIX$mount_source"
  if [ -z "$base_name" ]; then
    echo "$mount_target"
  else
    echo "$mount_target/$base_name"
  fi
}
function ViashDockerAutodetectMountArg {
  local abs_path=$(ViashAbsolutePath "$1")
  local mount_source
  local base_name
  if [ -d "$abs_path" ]; then
    mount_source="$abs_path"
    base_name=""
  else
    mount_source=`dirname "$abs_path"`
    base_name=`basename "$abs_path"`
  fi
  local mount_target="$VIASH_DOCKER_AUTOMOUNT_PREFIX$mount_source"
  ViashDebug "ViashDockerAutodetectMountArg $1 -> $mount_source -> $mount_target"
  echo "--volume=\"$mount_source:$mount_target\""
}
function ViashDockerStripAutomount {
  local abs_path=$(ViashAbsolutePath "$1")
  echo "${abs_path#$VIASH_DOCKER_AUTOMOUNT_PREFIX}"
}
# initialise variables
VIASH_DIRECTORY_MOUNTS=()

# configure default docker automount prefix if it is unset
if [ -z "${VIASH_DOCKER_AUTOMOUNT_PREFIX+x}" ]; then
  VIASH_DOCKER_AUTOMOUNT_PREFIX="/viash_automount"
fi

# initialise docker variables
VIASH_DOCKER_RUN_ARGS=(-i --rm)

# initialise array
VIASH_POSITIONAL_ARGS=''

while [[ $# -gt 0 ]]; do
    case "$1" in
        -h|--help)
            ViashHelp
            exit
            ;;
        ---v|---verbose)
            let "VIASH_VERBOSITY=VIASH_VERBOSITY+1"
            shift 1
            ;;
        ---verbosity)
            VIASH_VERBOSITY="$2"
            shift 2
            ;;
        ---verbosity=*)
            VIASH_VERBOSITY="$(ViashRemoveFlags "$1")"
            shift 1
            ;;
        --version)
            echo "lofreq_call main"
            exit
            ;;
        --input)
            [ -n "$VIASH_PAR_INPUT" ] && ViashError Bad arguments for option \'--input\': \'$VIASH_PAR_INPUT\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_INPUT="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --input. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --input=*)
            [ -n "$VIASH_PAR_INPUT" ] && ViashError Bad arguments for option \'--input=*\': \'$VIASH_PAR_INPUT\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_INPUT=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        --input_bai)
            [ -n "$VIASH_PAR_INPUT_BAI" ] && ViashError Bad arguments for option \'--input_bai\': \'$VIASH_PAR_INPUT_BAI\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_INPUT_BAI="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --input_bai. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --input_bai=*)
            [ -n "$VIASH_PAR_INPUT_BAI" ] && ViashError Bad arguments for option \'--input_bai=*\': \'$VIASH_PAR_INPUT_BAI\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_INPUT_BAI=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        --ref)
            [ -n "$VIASH_PAR_REF" ] && ViashError Bad arguments for option \'--ref\': \'$VIASH_PAR_REF\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_REF="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --ref. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --ref=*)
            [ -n "$VIASH_PAR_REF" ] && ViashError Bad arguments for option \'--ref=*\': \'$VIASH_PAR_REF\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_REF=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -f)
            [ -n "$VIASH_PAR_REF" ] && ViashError Bad arguments for option \'-f\': \'$VIASH_PAR_REF\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_REF="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -f. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --out)
            [ -n "$VIASH_PAR_OUT" ] && ViashError Bad arguments for option \'--out\': \'$VIASH_PAR_OUT\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_OUT="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --out. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --out=*)
            [ -n "$VIASH_PAR_OUT" ] && ViashError Bad arguments for option \'--out=*\': \'$VIASH_PAR_OUT\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_OUT=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -o)
            [ -n "$VIASH_PAR_OUT" ] && ViashError Bad arguments for option \'-o\': \'$VIASH_PAR_OUT\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_OUT="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -o. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --region)
            [ -n "$VIASH_PAR_REGION" ] && ViashError Bad arguments for option \'--region\': \'$VIASH_PAR_REGION\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_REGION="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --region. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --region=*)
            [ -n "$VIASH_PAR_REGION" ] && ViashError Bad arguments for option \'--region=*\': \'$VIASH_PAR_REGION\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_REGION=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -r)
            [ -n "$VIASH_PAR_REGION" ] && ViashError Bad arguments for option \'-r\': \'$VIASH_PAR_REGION\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_REGION="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -r. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --bed)
            [ -n "$VIASH_PAR_BED" ] && ViashError Bad arguments for option \'--bed\': \'$VIASH_PAR_BED\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_BED="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --bed. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --bed=*)
            [ -n "$VIASH_PAR_BED" ] && ViashError Bad arguments for option \'--bed=*\': \'$VIASH_PAR_BED\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_BED=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -l)
            [ -n "$VIASH_PAR_BED" ] && ViashError Bad arguments for option \'-l\': \'$VIASH_PAR_BED\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_BED="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -l. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --min_bq)
            [ -n "$VIASH_PAR_MIN_BQ" ] && ViashError Bad arguments for option \'--min_bq\': \'$VIASH_PAR_MIN_BQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_BQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --min_bq. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --min_bq=*)
            [ -n "$VIASH_PAR_MIN_BQ" ] && ViashError Bad arguments for option \'--min_bq=*\': \'$VIASH_PAR_MIN_BQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_BQ=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -q)
            [ -n "$VIASH_PAR_MIN_BQ" ] && ViashError Bad arguments for option \'-q\': \'$VIASH_PAR_MIN_BQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_BQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -q. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --min_alt_bq)
            [ -n "$VIASH_PAR_MIN_ALT_BQ" ] && ViashError Bad arguments for option \'--min_alt_bq\': \'$VIASH_PAR_MIN_ALT_BQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_ALT_BQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --min_alt_bq. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --min_alt_bq=*)
            [ -n "$VIASH_PAR_MIN_ALT_BQ" ] && ViashError Bad arguments for option \'--min_alt_bq=*\': \'$VIASH_PAR_MIN_ALT_BQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_ALT_BQ=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -Q)
            [ -n "$VIASH_PAR_MIN_ALT_BQ" ] && ViashError Bad arguments for option \'-Q\': \'$VIASH_PAR_MIN_ALT_BQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_ALT_BQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -Q. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --def_alt_bq)
            [ -n "$VIASH_PAR_DEF_ALT_BQ" ] && ViashError Bad arguments for option \'--def_alt_bq\': \'$VIASH_PAR_DEF_ALT_BQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_DEF_ALT_BQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --def_alt_bq. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --def_alt_bq=*)
            [ -n "$VIASH_PAR_DEF_ALT_BQ" ] && ViashError Bad arguments for option \'--def_alt_bq=*\': \'$VIASH_PAR_DEF_ALT_BQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_DEF_ALT_BQ=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -R)
            [ -n "$VIASH_PAR_DEF_ALT_BQ" ] && ViashError Bad arguments for option \'-R\': \'$VIASH_PAR_DEF_ALT_BQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_DEF_ALT_BQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -R. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --min_jq)
            [ -n "$VIASH_PAR_MIN_JQ" ] && ViashError Bad arguments for option \'--min_jq\': \'$VIASH_PAR_MIN_JQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_JQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --min_jq. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --min_jq=*)
            [ -n "$VIASH_PAR_MIN_JQ" ] && ViashError Bad arguments for option \'--min_jq=*\': \'$VIASH_PAR_MIN_JQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_JQ=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -j)
            [ -n "$VIASH_PAR_MIN_JQ" ] && ViashError Bad arguments for option \'-j\': \'$VIASH_PAR_MIN_JQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_JQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -j. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --min_alt_jq)
            [ -n "$VIASH_PAR_MIN_ALT_JQ" ] && ViashError Bad arguments for option \'--min_alt_jq\': \'$VIASH_PAR_MIN_ALT_JQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_ALT_JQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --min_alt_jq. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --min_alt_jq=*)
            [ -n "$VIASH_PAR_MIN_ALT_JQ" ] && ViashError Bad arguments for option \'--min_alt_jq=*\': \'$VIASH_PAR_MIN_ALT_JQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_ALT_JQ=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -J)
            [ -n "$VIASH_PAR_MIN_ALT_JQ" ] && ViashError Bad arguments for option \'-J\': \'$VIASH_PAR_MIN_ALT_JQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_ALT_JQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -J. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --def_alt_jq)
            [ -n "$VIASH_PAR_DEF_ALT_JQ" ] && ViashError Bad arguments for option \'--def_alt_jq\': \'$VIASH_PAR_DEF_ALT_JQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_DEF_ALT_JQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --def_alt_jq. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --def_alt_jq=*)
            [ -n "$VIASH_PAR_DEF_ALT_JQ" ] && ViashError Bad arguments for option \'--def_alt_jq=*\': \'$VIASH_PAR_DEF_ALT_JQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_DEF_ALT_JQ=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -K)
            [ -n "$VIASH_PAR_DEF_ALT_JQ" ] && ViashError Bad arguments for option \'-K\': \'$VIASH_PAR_DEF_ALT_JQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_DEF_ALT_JQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -K. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --no_baq)
            [ -n "$VIASH_PAR_NO_BAQ" ] && ViashError Bad arguments for option \'--no_baq\': \'$VIASH_PAR_NO_BAQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_NO_BAQ=true
            shift 1
            ;;
        -B)
            [ -n "$VIASH_PAR_NO_BAQ" ] && ViashError Bad arguments for option \'-B\': \'$VIASH_PAR_NO_BAQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_NO_BAQ=true
            shift 1
            ;;
        --no_idaq)
            [ -n "$VIASH_PAR_NO_IDAQ" ] && ViashError Bad arguments for option \'--no_idaq\': \'$VIASH_PAR_NO_IDAQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_NO_IDAQ=true
            shift 1
            ;;
        -A)
            [ -n "$VIASH_PAR_NO_IDAQ" ] && ViashError Bad arguments for option \'-A\': \'$VIASH_PAR_NO_IDAQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_NO_IDAQ=true
            shift 1
            ;;
        --del_baq)
            [ -n "$VIASH_PAR_DEL_BAQ" ] && ViashError Bad arguments for option \'--del_baq\': \'$VIASH_PAR_DEL_BAQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_DEL_BAQ=true
            shift 1
            ;;
        -D)
            [ -n "$VIASH_PAR_DEL_BAQ" ] && ViashError Bad arguments for option \'-D\': \'$VIASH_PAR_DEL_BAQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_DEL_BAQ=true
            shift 1
            ;;
        --no_ext_baq)
            [ -n "$VIASH_PAR_NO_EXT_BAQ" ] && ViashError Bad arguments for option \'--no_ext_baq\': \'$VIASH_PAR_NO_EXT_BAQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_NO_EXT_BAQ=true
            shift 1
            ;;
        -e)
            [ -n "$VIASH_PAR_NO_EXT_BAQ" ] && ViashError Bad arguments for option \'-e\': \'$VIASH_PAR_NO_EXT_BAQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_NO_EXT_BAQ=true
            shift 1
            ;;
        --min_mq)
            [ -n "$VIASH_PAR_MIN_MQ" ] && ViashError Bad arguments for option \'--min_mq\': \'$VIASH_PAR_MIN_MQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_MQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --min_mq. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --min_mq=*)
            [ -n "$VIASH_PAR_MIN_MQ" ] && ViashError Bad arguments for option \'--min_mq=*\': \'$VIASH_PAR_MIN_MQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_MQ=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -m)
            [ -n "$VIASH_PAR_MIN_MQ" ] && ViashError Bad arguments for option \'-m\': \'$VIASH_PAR_MIN_MQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_MQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -m. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --max_mq)
            [ -n "$VIASH_PAR_MAX_MQ" ] && ViashError Bad arguments for option \'--max_mq\': \'$VIASH_PAR_MAX_MQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MAX_MQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --max_mq. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --max_mq=*)
            [ -n "$VIASH_PAR_MAX_MQ" ] && ViashError Bad arguments for option \'--max_mq=*\': \'$VIASH_PAR_MAX_MQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MAX_MQ=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -M)
            [ -n "$VIASH_PAR_MAX_MQ" ] && ViashError Bad arguments for option \'-M\': \'$VIASH_PAR_MAX_MQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MAX_MQ="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -M. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --no_mq)
            [ -n "$VIASH_PAR_NO_MQ" ] && ViashError Bad arguments for option \'--no_mq\': \'$VIASH_PAR_NO_MQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_NO_MQ=true
            shift 1
            ;;
        -N)
            [ -n "$VIASH_PAR_NO_MQ" ] && ViashError Bad arguments for option \'-N\': \'$VIASH_PAR_NO_MQ\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_NO_MQ=true
            shift 1
            ;;
        --call_indels)
            [ -n "$VIASH_PAR_CALL_INDELS" ] && ViashError Bad arguments for option \'--call_indels\': \'$VIASH_PAR_CALL_INDELS\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_CALL_INDELS=true
            shift 1
            ;;
        --only_indels)
            [ -n "$VIASH_PAR_ONLY_INDELS" ] && ViashError Bad arguments for option \'--only_indels\': \'$VIASH_PAR_ONLY_INDELS\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_ONLY_INDELS=true
            shift 1
            ;;
        --src_qual)
            [ -n "$VIASH_PAR_SRC_QUAL" ] && ViashError Bad arguments for option \'--src_qual\': \'$VIASH_PAR_SRC_QUAL\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_SRC_QUAL=true
            shift 1
            ;;
        -s)
            [ -n "$VIASH_PAR_SRC_QUAL" ] && ViashError Bad arguments for option \'-s\': \'$VIASH_PAR_SRC_QUAL\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_SRC_QUAL=true
            shift 1
            ;;
        --ign_vcf)
            [ -n "$VIASH_PAR_IGN_VCF" ] && ViashError Bad arguments for option \'--ign_vcf\': \'$VIASH_PAR_IGN_VCF\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_IGN_VCF="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --ign_vcf. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --ign_vcf=*)
            [ -n "$VIASH_PAR_IGN_VCF" ] && ViashError Bad arguments for option \'--ign_vcf=*\': \'$VIASH_PAR_IGN_VCF\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_IGN_VCF=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -S)
            [ -n "$VIASH_PAR_IGN_VCF" ] && ViashError Bad arguments for option \'-S\': \'$VIASH_PAR_IGN_VCF\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_IGN_VCF="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -S. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --def_nm_q)
            [ -n "$VIASH_PAR_DEF_NM_Q" ] && ViashError Bad arguments for option \'--def_nm_q\': \'$VIASH_PAR_DEF_NM_Q\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_DEF_NM_Q="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --def_nm_q. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --def_nm_q=*)
            [ -n "$VIASH_PAR_DEF_NM_Q" ] && ViashError Bad arguments for option \'--def_nm_q=*\': \'$VIASH_PAR_DEF_NM_Q\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_DEF_NM_Q=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -T)
            [ -n "$VIASH_PAR_DEF_NM_Q" ] && ViashError Bad arguments for option \'-T\': \'$VIASH_PAR_DEF_NM_Q\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_DEF_NM_Q="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -T. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --sig)
            [ -n "$VIASH_PAR_SIG" ] && ViashError Bad arguments for option \'--sig\': \'$VIASH_PAR_SIG\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_SIG="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --sig. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --sig=*)
            [ -n "$VIASH_PAR_SIG" ] && ViashError Bad arguments for option \'--sig=*\': \'$VIASH_PAR_SIG\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_SIG=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -a)
            [ -n "$VIASH_PAR_SIG" ] && ViashError Bad arguments for option \'-a\': \'$VIASH_PAR_SIG\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_SIG="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -a. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --bonf)
            [ -n "$VIASH_PAR_BONF" ] && ViashError Bad arguments for option \'--bonf\': \'$VIASH_PAR_BONF\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_BONF="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --bonf. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --bonf=*)
            [ -n "$VIASH_PAR_BONF" ] && ViashError Bad arguments for option \'--bonf=*\': \'$VIASH_PAR_BONF\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_BONF=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -b)
            [ -n "$VIASH_PAR_BONF" ] && ViashError Bad arguments for option \'-b\': \'$VIASH_PAR_BONF\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_BONF="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -b. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --min_cov)
            [ -n "$VIASH_PAR_MIN_COV" ] && ViashError Bad arguments for option \'--min_cov\': \'$VIASH_PAR_MIN_COV\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_COV="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --min_cov. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --min_cov=*)
            [ -n "$VIASH_PAR_MIN_COV" ] && ViashError Bad arguments for option \'--min_cov=*\': \'$VIASH_PAR_MIN_COV\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_COV=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -C)
            [ -n "$VIASH_PAR_MIN_COV" ] && ViashError Bad arguments for option \'-C\': \'$VIASH_PAR_MIN_COV\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MIN_COV="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -C. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --max_depth)
            [ -n "$VIASH_PAR_MAX_DEPTH" ] && ViashError Bad arguments for option \'--max_depth\': \'$VIASH_PAR_MAX_DEPTH\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MAX_DEPTH="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to --max_depth. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --max_depth=*)
            [ -n "$VIASH_PAR_MAX_DEPTH" ] && ViashError Bad arguments for option \'--max_depth=*\': \'$VIASH_PAR_MAX_DEPTH\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MAX_DEPTH=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        -d)
            [ -n "$VIASH_PAR_MAX_DEPTH" ] && ViashError Bad arguments for option \'-d\': \'$VIASH_PAR_MAX_DEPTH\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_MAX_DEPTH="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to -d. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        --illumina_13)
            [ -n "$VIASH_PAR_ILLUMINA_13" ] && ViashError Bad arguments for option \'--illumina_13\': \'$VIASH_PAR_ILLUMINA_13\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_ILLUMINA_13=true
            shift 1
            ;;
        --use_orphan)
            [ -n "$VIASH_PAR_USE_ORPHAN" ] && ViashError Bad arguments for option \'--use_orphan\': \'$VIASH_PAR_USE_ORPHAN\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_USE_ORPHAN=true
            shift 1
            ;;
        --plp_summary_only)
            [ -n "$VIASH_PAR_PLP_SUMMARY_ONLY" ] && ViashError Bad arguments for option \'--plp_summary_only\': \'$VIASH_PAR_PLP_SUMMARY_ONLY\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_PLP_SUMMARY_ONLY=true
            shift 1
            ;;
        --no_default_filter)
            [ -n "$VIASH_PAR_NO_DEFAULT_FILTER" ] && ViashError Bad arguments for option \'--no_default_filter\': \'$VIASH_PAR_NO_DEFAULT_FILTER\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_NO_DEFAULT_FILTER=true
            shift 1
            ;;
        --force_overwrite)
            [ -n "$VIASH_PAR_FORCE_OVERWRITE" ] && ViashError Bad arguments for option \'--force_overwrite\': \'$VIASH_PAR_FORCE_OVERWRITE\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_FORCE_OVERWRITE=true
            shift 1
            ;;
        --verbose)
            [ -n "$VIASH_PAR_VERBOSE" ] && ViashError Bad arguments for option \'--verbose\': \'$VIASH_PAR_VERBOSE\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_VERBOSE=true
            shift 1
            ;;
        --debug)
            [ -n "$VIASH_PAR_DEBUG" ] && ViashError Bad arguments for option \'--debug\': \'$VIASH_PAR_DEBUG\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_PAR_DEBUG=true
            shift 1
            ;;
        ---engine)
            VIASH_ENGINE_ID="$2"
            shift 2
            ;;
        ---engine=*)
            VIASH_ENGINE_ID="$(ViashRemoveFlags "$1")"
            shift 1
            ;;
        ---setup)
            VIASH_MODE='setup'
            VIASH_SETUP_STRATEGY="$2"
            shift 2
            ;;
        ---setup=*)
            VIASH_MODE='setup'
            VIASH_SETUP_STRATEGY="$(ViashRemoveFlags "$1")"
            shift 1
            ;;
        ---dockerfile)
            VIASH_MODE='dockerfile'
            shift 1
            ;;
        ---docker_run_args)
            VIASH_DOCKER_RUN_ARGS+=("$2")
            shift 2
            ;;
        ---docker_run_args=*)
            VIASH_DOCKER_RUN_ARGS+=("$(ViashRemoveFlags "$1")")
            shift 1
            ;;
        ---docker_image_id)
            VIASH_MODE='docker_image_id'
            shift 1
            ;;
        ---debug)
            VIASH_MODE='debug'
            shift 1
            ;;
        ---cpus)
            [ -n "$VIASH_META_CPUS" ] && ViashError Bad arguments for option \'---cpus\': \'$VIASH_META_CPUS\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_META_CPUS="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to ---cpus. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        ---cpus=*)
            [ -n "$VIASH_META_CPUS" ] && ViashError Bad arguments for option \'---cpus=*\': \'$VIASH_META_CPUS\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_META_CPUS=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        ---memory)
            [ -n "$VIASH_META_MEMORY" ] && ViashError Bad arguments for option \'---memory\': \'$VIASH_META_MEMORY\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_META_MEMORY="$2"
            [ $# -lt 2 ] && ViashError Not enough arguments passed to ---memory. Use "--help" to get more information on the parameters. && exit 1
            shift 2
            ;;
        ---memory=*)
            [ -n "$VIASH_META_MEMORY" ] && ViashError Bad arguments for option \'---memory=*\': \'$VIASH_META_MEMORY\' \& \'$2\' - you should provide exactly one argument for this option. && exit 1
            VIASH_META_MEMORY=$(ViashRemoveFlags "$1")
            shift 1
            ;;
        *)  # positional arg or unknown option
            # since the positional args will be eval'd, can we always quote, instead of using ViashQuote
            VIASH_POSITIONAL_ARGS="$VIASH_POSITIONAL_ARGS '$1'"
            [[ $1 == -* ]] && ViashWarning $1 looks like a parameter but is not a defined parameter and will instead be treated as a positional argument. Use "--help" to get more information on the parameters.
            shift # past argument
            ;;
    esac
done

# parse positional parameters
eval set -- $VIASH_POSITIONAL_ARGS


if   [ "$VIASH_ENGINE_ID" == "native" ]  ; then
  VIASH_ENGINE_TYPE='native'
elif   [ "$VIASH_ENGINE_ID" == "docker" ]  ; then
  VIASH_ENGINE_TYPE='docker'
else
  ViashError "Engine '$VIASH_ENGINE_ID' is not recognized. Options are: docker, native."
  exit 1
fi

if [[ "$VIASH_ENGINE_TYPE" == "docker" ]]; then
  # check if docker is installed properly
  ViashDockerInstallationCheck

  # determine docker image id
  if [[ "$VIASH_ENGINE_ID" == 'docker' ]]; then
    VIASH_DOCKER_IMAGE_ID='images.viash-hub.com/vsh/biobox/lofreq/lofreq_call:main'
  fi

  # print dockerfile
  if [ "$VIASH_MODE" == "dockerfile" ]; then
    ViashDockerfile "$VIASH_ENGINE_ID"
    exit 0

  elif [ "$VIASH_MODE" == "docker_image_id" ]; then
    echo "$VIASH_DOCKER_IMAGE_ID"
    exit 0
  
  # enter docker container
  elif [[ "$VIASH_MODE" == "debug" ]]; then
    VIASH_CMD="docker run --entrypoint=bash ${VIASH_DOCKER_RUN_ARGS[@]} -v '$(pwd)':/pwd --workdir /pwd -t $VIASH_DOCKER_IMAGE_ID"
    ViashNotice "+ $VIASH_CMD"
    eval $VIASH_CMD
    exit 

  # build docker image
  elif [ "$VIASH_MODE" == "setup" ]; then
    ViashDockerSetup "$VIASH_DOCKER_IMAGE_ID" "$VIASH_SETUP_STRATEGY"
    ViashDockerCheckCommands "$VIASH_DOCKER_IMAGE_ID" 'ps' 'bash'
    exit 0
  fi

  # check if docker image exists
  ViashDockerSetup "$VIASH_DOCKER_IMAGE_ID" ifneedbepullelsecachedbuild
  ViashDockerCheckCommands "$VIASH_DOCKER_IMAGE_ID" 'ps' 'bash'
fi

# setting computational defaults

# helper function for parsing memory strings
function ViashMemoryAsBytes {
  local memory=`echo "$1" | tr '[:upper:]' '[:lower:]' | tr -d '[:space:]'`
  local memory_regex='^([0-9]+)([kmgtp]i?b?|b)$'
  if [[ $memory =~ $memory_regex ]]; then
    local number=${memory/[^0-9]*/}
    local symbol=${memory/*[0-9]/}
    
    case $symbol in
      b)      memory_b=$number ;;
      kb|k)   memory_b=$(( $number * 1000 )) ;;
      mb|m)   memory_b=$(( $number * 1000 * 1000 )) ;;
      gb|g)   memory_b=$(( $number * 1000 * 1000 * 1000 )) ;;
      tb|t)   memory_b=$(( $number * 1000 * 1000 * 1000 * 1000 )) ;;
      pb|p)   memory_b=$(( $number * 1000 * 1000 * 1000 * 1000 * 1000 )) ;;
      kib|ki)   memory_b=$(( $number * 1024 )) ;;
      mib|mi)   memory_b=$(( $number * 1024 * 1024 )) ;;
      gib|gi)   memory_b=$(( $number * 1024 * 1024 * 1024 )) ;;
      tib|ti)   memory_b=$(( $number * 1024 * 1024 * 1024 * 1024 )) ;;
      pib|pi)   memory_b=$(( $number * 1024 * 1024 * 1024 * 1024 * 1024 )) ;;
    esac
    echo "$memory_b"
  fi
}
# compute memory in different units
if [ ! -z ${VIASH_META_MEMORY+x} ]; then
  VIASH_META_MEMORY_B=`ViashMemoryAsBytes $VIASH_META_MEMORY`
  # do not define other variables if memory_b is an empty string
  if [ ! -z "$VIASH_META_MEMORY_B" ]; then
    VIASH_META_MEMORY_KB=$(( ($VIASH_META_MEMORY_B+999) / 1000 ))
    VIASH_META_MEMORY_MB=$(( ($VIASH_META_MEMORY_KB+999) / 1000 ))
    VIASH_META_MEMORY_GB=$(( ($VIASH_META_MEMORY_MB+999) / 1000 ))
    VIASH_META_MEMORY_TB=$(( ($VIASH_META_MEMORY_GB+999) / 1000 ))
    VIASH_META_MEMORY_PB=$(( ($VIASH_META_MEMORY_TB+999) / 1000 ))
    VIASH_META_MEMORY_KIB=$(( ($VIASH_META_MEMORY_B+1023) / 1024 ))
    VIASH_META_MEMORY_MIB=$(( ($VIASH_META_MEMORY_KIB+1023) / 1024 ))
    VIASH_META_MEMORY_GIB=$(( ($VIASH_META_MEMORY_MIB+1023) / 1024 ))
    VIASH_META_MEMORY_TIB=$(( ($VIASH_META_MEMORY_GIB+1023) / 1024 ))
    VIASH_META_MEMORY_PIB=$(( ($VIASH_META_MEMORY_TIB+1023) / 1024 ))
  else
    # unset memory if string is empty
    unset $VIASH_META_MEMORY_B
  fi
fi
# unset nproc if string is empty
if [ -z "$VIASH_META_CPUS" ]; then
  unset $VIASH_META_CPUS
fi


# check whether required parameters exist
if [ -z ${VIASH_PAR_INPUT+x} ]; then
  ViashError '--input' is a required argument. Use "--help" to get more information on the parameters.
  exit 1
fi
if [ -z ${VIASH_PAR_INPUT_BAI+x} ]; then
  ViashError '--input_bai' is a required argument. Use "--help" to get more information on the parameters.
  exit 1
fi
if [ -z ${VIASH_PAR_REF+x} ]; then
  ViashError '--ref' is a required argument. Use "--help" to get more information on the parameters.
  exit 1
fi
if [ -z ${VIASH_PAR_OUT+x} ]; then
  ViashError '--out' is a required argument. Use "--help" to get more information on the parameters.
  exit 1
fi
if [ -z ${VIASH_META_NAME+x} ]; then
  ViashError 'name' is a required argument. Use "--help" to get more information on the parameters.
  exit 1
fi
if [ -z ${VIASH_META_FUNCTIONALITY_NAME+x} ]; then
  ViashError 'functionality_name' is a required argument. Use "--help" to get more information on the parameters.
  exit 1
fi
if [ -z ${VIASH_META_RESOURCES_DIR+x} ]; then
  ViashError 'resources_dir' is a required argument. Use "--help" to get more information on the parameters.
  exit 1
fi
if [ -z ${VIASH_META_EXECUTABLE+x} ]; then
  ViashError 'executable' is a required argument. Use "--help" to get more information on the parameters.
  exit 1
fi
if [ -z ${VIASH_META_CONFIG+x} ]; then
  ViashError 'config' is a required argument. Use "--help" to get more information on the parameters.
  exit 1
fi
if [ -z ${VIASH_META_TEMP_DIR+x} ]; then
  ViashError 'temp_dir' is a required argument. Use "--help" to get more information on the parameters.
  exit 1
fi

# filling in defaults
if [ -z ${VIASH_PAR_NO_BAQ+x} ]; then
  VIASH_PAR_NO_BAQ="false"
fi
if [ -z ${VIASH_PAR_NO_IDAQ+x} ]; then
  VIASH_PAR_NO_IDAQ="false"
fi
if [ -z ${VIASH_PAR_DEL_BAQ+x} ]; then
  VIASH_PAR_DEL_BAQ="false"
fi
if [ -z ${VIASH_PAR_NO_EXT_BAQ+x} ]; then
  VIASH_PAR_NO_EXT_BAQ="false"
fi
if [ -z ${VIASH_PAR_NO_MQ+x} ]; then
  VIASH_PAR_NO_MQ="false"
fi
if [ -z ${VIASH_PAR_CALL_INDELS+x} ]; then
  VIASH_PAR_CALL_INDELS="false"
fi
if [ -z ${VIASH_PAR_ONLY_INDELS+x} ]; then
  VIASH_PAR_ONLY_INDELS="false"
fi
if [ -z ${VIASH_PAR_SRC_QUAL+x} ]; then
  VIASH_PAR_SRC_QUAL="false"
fi
if [ -z ${VIASH_PAR_ILLUMINA_13+x} ]; then
  VIASH_PAR_ILLUMINA_13="false"
fi
if [ -z ${VIASH_PAR_USE_ORPHAN+x} ]; then
  VIASH_PAR_USE_ORPHAN="false"
fi
if [ -z ${VIASH_PAR_PLP_SUMMARY_ONLY+x} ]; then
  VIASH_PAR_PLP_SUMMARY_ONLY="false"
fi
if [ -z ${VIASH_PAR_NO_DEFAULT_FILTER+x} ]; then
  VIASH_PAR_NO_DEFAULT_FILTER="false"
fi
if [ -z ${VIASH_PAR_FORCE_OVERWRITE+x} ]; then
  VIASH_PAR_FORCE_OVERWRITE="false"
fi
if [ -z ${VIASH_PAR_VERBOSE+x} ]; then
  VIASH_PAR_VERBOSE="false"
fi
if [ -z ${VIASH_PAR_DEBUG+x} ]; then
  VIASH_PAR_DEBUG="false"
fi

# check whether required files exist
if [ ! -z "$VIASH_PAR_INPUT" ] && [ ! -e "$VIASH_PAR_INPUT" ]; then
  ViashError "Input file '$VIASH_PAR_INPUT' does not exist."
  exit 1
fi
if [ ! -z "$VIASH_PAR_INPUT_BAI" ] && [ ! -e "$VIASH_PAR_INPUT_BAI" ]; then
  ViashError "Input file '$VIASH_PAR_INPUT_BAI' does not exist."
  exit 1
fi
if [ ! -z "$VIASH_PAR_REF" ] && [ ! -e "$VIASH_PAR_REF" ]; then
  ViashError "Input file '$VIASH_PAR_REF' does not exist."
  exit 1
fi
if [ ! -z "$VIASH_PAR_BED" ] && [ ! -e "$VIASH_PAR_BED" ]; then
  ViashError "Input file '$VIASH_PAR_BED' does not exist."
  exit 1
fi
if [ ! -z "$VIASH_PAR_IGN_VCF" ] && [ ! -e "$VIASH_PAR_IGN_VCF" ]; then
  ViashError "Input file '$VIASH_PAR_IGN_VCF' does not exist."
  exit 1
fi

# check whether parameters values are of the right type
if [[ -n "$VIASH_PAR_MIN_BQ" ]]; then
  if ! [[ "$VIASH_PAR_MIN_BQ" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError '--min_bq' has to be an integer. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_MIN_ALT_BQ" ]]; then
  if ! [[ "$VIASH_PAR_MIN_ALT_BQ" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError '--min_alt_bq' has to be an integer. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_DEF_ALT_BQ" ]]; then
  if ! [[ "$VIASH_PAR_DEF_ALT_BQ" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError '--def_alt_bq' has to be an integer. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_MIN_JQ" ]]; then
  if ! [[ "$VIASH_PAR_MIN_JQ" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError '--min_jq' has to be an integer. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_MIN_ALT_JQ" ]]; then
  if ! [[ "$VIASH_PAR_MIN_ALT_JQ" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError '--min_alt_jq' has to be an integer. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_DEF_ALT_JQ" ]]; then
  if ! [[ "$VIASH_PAR_DEF_ALT_JQ" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError '--def_alt_jq' has to be an integer. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_NO_BAQ" ]]; then
  if ! [[ "$VIASH_PAR_NO_BAQ" =~ ^(true|True|TRUE|false|False|FALSE|yes|Yes|YES|no|No|NO)$ ]]; then
    ViashError '--no_baq' has to be a boolean_true. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_NO_IDAQ" ]]; then
  if ! [[ "$VIASH_PAR_NO_IDAQ" =~ ^(true|True|TRUE|false|False|FALSE|yes|Yes|YES|no|No|NO)$ ]]; then
    ViashError '--no_idaq' has to be a boolean_true. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_DEL_BAQ" ]]; then
  if ! [[ "$VIASH_PAR_DEL_BAQ" =~ ^(true|True|TRUE|false|False|FALSE|yes|Yes|YES|no|No|NO)$ ]]; then
    ViashError '--del_baq' has to be a boolean_true. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_NO_EXT_BAQ" ]]; then
  if ! [[ "$VIASH_PAR_NO_EXT_BAQ" =~ ^(true|True|TRUE|false|False|FALSE|yes|Yes|YES|no|No|NO)$ ]]; then
    ViashError '--no_ext_baq' has to be a boolean_true. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_MIN_MQ" ]]; then
  if ! [[ "$VIASH_PAR_MIN_MQ" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError '--min_mq' has to be an integer. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_MAX_MQ" ]]; then
  if ! [[ "$VIASH_PAR_MAX_MQ" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError '--max_mq' has to be an integer. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_NO_MQ" ]]; then
  if ! [[ "$VIASH_PAR_NO_MQ" =~ ^(true|True|TRUE|false|False|FALSE|yes|Yes|YES|no|No|NO)$ ]]; then
    ViashError '--no_mq' has to be a boolean_true. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_CALL_INDELS" ]]; then
  if ! [[ "$VIASH_PAR_CALL_INDELS" =~ ^(true|True|TRUE|false|False|FALSE|yes|Yes|YES|no|No|NO)$ ]]; then
    ViashError '--call_indels' has to be a boolean_true. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_ONLY_INDELS" ]]; then
  if ! [[ "$VIASH_PAR_ONLY_INDELS" =~ ^(true|True|TRUE|false|False|FALSE|yes|Yes|YES|no|No|NO)$ ]]; then
    ViashError '--only_indels' has to be a boolean_true. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_SRC_QUAL" ]]; then
  if ! [[ "$VIASH_PAR_SRC_QUAL" =~ ^(true|True|TRUE|false|False|FALSE|yes|Yes|YES|no|No|NO)$ ]]; then
    ViashError '--src_qual' has to be a boolean_true. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_DEF_NM_Q" ]]; then
  if ! [[ "$VIASH_PAR_DEF_NM_Q" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError '--def_nm_q' has to be an integer. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_SIG" ]]; then
  if ! [[ "$VIASH_PAR_SIG" =~ ^[-+]?(\.[0-9]+|[0-9]+(\.[0-9]*)?)([eE][-+]?[0-9]+)?$ ]]; then
    ViashError '--sig' has to be a double. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_MIN_COV" ]]; then
  if ! [[ "$VIASH_PAR_MIN_COV" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError '--min_cov' has to be an integer. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_MAX_DEPTH" ]]; then
  if ! [[ "$VIASH_PAR_MAX_DEPTH" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError '--max_depth' has to be an integer. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_ILLUMINA_13" ]]; then
  if ! [[ "$VIASH_PAR_ILLUMINA_13" =~ ^(true|True|TRUE|false|False|FALSE|yes|Yes|YES|no|No|NO)$ ]]; then
    ViashError '--illumina_13' has to be a boolean_true. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_USE_ORPHAN" ]]; then
  if ! [[ "$VIASH_PAR_USE_ORPHAN" =~ ^(true|True|TRUE|false|False|FALSE|yes|Yes|YES|no|No|NO)$ ]]; then
    ViashError '--use_orphan' has to be a boolean_true. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_PLP_SUMMARY_ONLY" ]]; then
  if ! [[ "$VIASH_PAR_PLP_SUMMARY_ONLY" =~ ^(true|True|TRUE|false|False|FALSE|yes|Yes|YES|no|No|NO)$ ]]; then
    ViashError '--plp_summary_only' has to be a boolean_true. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_NO_DEFAULT_FILTER" ]]; then
  if ! [[ "$VIASH_PAR_NO_DEFAULT_FILTER" =~ ^(true|True|TRUE|false|False|FALSE|yes|Yes|YES|no|No|NO)$ ]]; then
    ViashError '--no_default_filter' has to be a boolean_true. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_FORCE_OVERWRITE" ]]; then
  if ! [[ "$VIASH_PAR_FORCE_OVERWRITE" =~ ^(true|True|TRUE|false|False|FALSE|yes|Yes|YES|no|No|NO)$ ]]; then
    ViashError '--force_overwrite' has to be a boolean_true. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_VERBOSE" ]]; then
  if ! [[ "$VIASH_PAR_VERBOSE" =~ ^(true|True|TRUE|false|False|FALSE|yes|Yes|YES|no|No|NO)$ ]]; then
    ViashError '--verbose' has to be a boolean_true. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_PAR_DEBUG" ]]; then
  if ! [[ "$VIASH_PAR_DEBUG" =~ ^(true|True|TRUE|false|False|FALSE|yes|Yes|YES|no|No|NO)$ ]]; then
    ViashError '--debug' has to be a boolean_true. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_META_CPUS" ]]; then
  if ! [[ "$VIASH_META_CPUS" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError 'cpus' has to be an integer. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_META_MEMORY_B" ]]; then
  if ! [[ "$VIASH_META_MEMORY_B" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError 'memory_b' has to be a long. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_META_MEMORY_KB" ]]; then
  if ! [[ "$VIASH_META_MEMORY_KB" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError 'memory_kb' has to be a long. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_META_MEMORY_MB" ]]; then
  if ! [[ "$VIASH_META_MEMORY_MB" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError 'memory_mb' has to be a long. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_META_MEMORY_GB" ]]; then
  if ! [[ "$VIASH_META_MEMORY_GB" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError 'memory_gb' has to be a long. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_META_MEMORY_TB" ]]; then
  if ! [[ "$VIASH_META_MEMORY_TB" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError 'memory_tb' has to be a long. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_META_MEMORY_PB" ]]; then
  if ! [[ "$VIASH_META_MEMORY_PB" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError 'memory_pb' has to be a long. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_META_MEMORY_KIB" ]]; then
  if ! [[ "$VIASH_META_MEMORY_KIB" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError 'memory_kib' has to be a long. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_META_MEMORY_MIB" ]]; then
  if ! [[ "$VIASH_META_MEMORY_MIB" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError 'memory_mib' has to be a long. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_META_MEMORY_GIB" ]]; then
  if ! [[ "$VIASH_META_MEMORY_GIB" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError 'memory_gib' has to be a long. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_META_MEMORY_TIB" ]]; then
  if ! [[ "$VIASH_META_MEMORY_TIB" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError 'memory_tib' has to be a long. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi
if [[ -n "$VIASH_META_MEMORY_PIB" ]]; then
  if ! [[ "$VIASH_META_MEMORY_PIB" =~ ^[-+]?[0-9]+$ ]]; then
    ViashError 'memory_pib' has to be a long. Use "--help" to get more information on the parameters.
    exit 1
  fi
fi

# create parent directories of output files, if so desired
if [ ! -z "$VIASH_PAR_OUT" ] && [ ! -d "$(dirname "$VIASH_PAR_OUT")" ]; then
  mkdir -p "$(dirname "$VIASH_PAR_OUT")"
fi

if  [ "$VIASH_ENGINE_ID" == "native" ]  ; then
  if [ "$VIASH_MODE" == "run" ]; then
    VIASH_CMD="bash"
  else
    ViashError "Engine '$VIASH_ENGINE_ID' does not support mode '$VIASH_MODE'."
    exit 1
  fi
fi

if [[ "$VIASH_ENGINE_TYPE" == "docker" ]]; then
  # detect volumes from file arguments
  VIASH_CHOWN_VARS=()
if [ ! -z "$VIASH_PAR_INPUT" ]; then
  VIASH_DIRECTORY_MOUNTS+=( "$(ViashDockerAutodetectMountArg "$VIASH_PAR_INPUT")" )
  VIASH_PAR_INPUT=$(ViashDockerAutodetectMount "$VIASH_PAR_INPUT")
fi
if [ ! -z "$VIASH_PAR_INPUT_BAI" ]; then
  VIASH_DIRECTORY_MOUNTS+=( "$(ViashDockerAutodetectMountArg "$VIASH_PAR_INPUT_BAI")" )
  VIASH_PAR_INPUT_BAI=$(ViashDockerAutodetectMount "$VIASH_PAR_INPUT_BAI")
fi
if [ ! -z "$VIASH_PAR_REF" ]; then
  VIASH_DIRECTORY_MOUNTS+=( "$(ViashDockerAutodetectMountArg "$VIASH_PAR_REF")" )
  VIASH_PAR_REF=$(ViashDockerAutodetectMount "$VIASH_PAR_REF")
fi
if [ ! -z "$VIASH_PAR_OUT" ]; then
  VIASH_DIRECTORY_MOUNTS+=( "$(ViashDockerAutodetectMountArg "$VIASH_PAR_OUT")" )
  VIASH_PAR_OUT=$(ViashDockerAutodetectMount "$VIASH_PAR_OUT")
  VIASH_CHOWN_VARS+=( "$VIASH_PAR_OUT" )
fi
if [ ! -z "$VIASH_PAR_BED" ]; then
  VIASH_DIRECTORY_MOUNTS+=( "$(ViashDockerAutodetectMountArg "$VIASH_PAR_BED")" )
  VIASH_PAR_BED=$(ViashDockerAutodetectMount "$VIASH_PAR_BED")
fi
if [ ! -z "$VIASH_PAR_IGN_VCF" ]; then
  VIASH_DIRECTORY_MOUNTS+=( "$(ViashDockerAutodetectMountArg "$VIASH_PAR_IGN_VCF")" )
  VIASH_PAR_IGN_VCF=$(ViashDockerAutodetectMount "$VIASH_PAR_IGN_VCF")
fi
if [ ! -z "$VIASH_META_RESOURCES_DIR" ]; then
  VIASH_DIRECTORY_MOUNTS+=( "$(ViashDockerAutodetectMountArg "$VIASH_META_RESOURCES_DIR")" )
  VIASH_META_RESOURCES_DIR=$(ViashDockerAutodetectMount "$VIASH_META_RESOURCES_DIR")
fi
if [ ! -z "$VIASH_META_EXECUTABLE" ]; then
  VIASH_DIRECTORY_MOUNTS+=( "$(ViashDockerAutodetectMountArg "$VIASH_META_EXECUTABLE")" )
  VIASH_META_EXECUTABLE=$(ViashDockerAutodetectMount "$VIASH_META_EXECUTABLE")
fi
if [ ! -z "$VIASH_META_CONFIG" ]; then
  VIASH_DIRECTORY_MOUNTS+=( "$(ViashDockerAutodetectMountArg "$VIASH_META_CONFIG")" )
  VIASH_META_CONFIG=$(ViashDockerAutodetectMount "$VIASH_META_CONFIG")
fi
if [ ! -z "$VIASH_META_TEMP_DIR" ]; then
  VIASH_DIRECTORY_MOUNTS+=( "$(ViashDockerAutodetectMountArg "$VIASH_META_TEMP_DIR")" )
  VIASH_META_TEMP_DIR=$(ViashDockerAutodetectMount "$VIASH_META_TEMP_DIR")
fi
  
  # get unique mounts
  VIASH_UNIQUE_MOUNTS=($(for val in "${VIASH_DIRECTORY_MOUNTS[@]}"; do echo "$val"; done | sort -u))
fi

if [[ "$VIASH_ENGINE_TYPE" == "docker" ]]; then
  # change file ownership
  function ViashPerformChown {
    if (( ${#VIASH_CHOWN_VARS[@]} )); then
      set +e
      VIASH_CMD="docker run --entrypoint=bash --rm ${VIASH_UNIQUE_MOUNTS[@]} $VIASH_DOCKER_IMAGE_ID -c 'chown $(id -u):$(id -g) --silent --recursive ${VIASH_CHOWN_VARS[@]}'"
      ViashDebug "+ $VIASH_CMD"
      eval $VIASH_CMD
      set -e
    fi
  }
  trap ViashPerformChown EXIT
fi

if [[ "$VIASH_ENGINE_TYPE" == "docker" ]]; then
  # helper function for filling in extra docker args
  if [ ! -z "$VIASH_META_MEMORY_B" ]; then
    VIASH_DOCKER_RUN_ARGS+=("--memory=${VIASH_META_MEMORY_B}")
  fi
  if [ ! -z "$VIASH_META_CPUS" ]; then
    VIASH_DOCKER_RUN_ARGS+=("--cpus=${VIASH_META_CPUS}")
  fi
fi

if [[ "$VIASH_ENGINE_TYPE" == "docker" ]]; then
  VIASH_CMD="docker run --entrypoint=bash ${VIASH_DOCKER_RUN_ARGS[@]} ${VIASH_UNIQUE_MOUNTS[@]} $VIASH_DOCKER_IMAGE_ID"
fi


# set dependency paths


ViashDebug "Running command: $(echo $VIASH_CMD)"
cat << VIASHEOF | eval $VIASH_CMD
set -e
tempscript=\$(mktemp "$VIASH_META_TEMP_DIR/viash-run-lofreq_call-XXXXXX").sh
function clean_up {
  rm "\$tempscript"
}
function interrupt {
  echo -e "\nCTRL-C Pressed..."
  exit 1
}
trap clean_up EXIT
trap interrupt INT SIGINT
cat > "\$tempscript" << 'VIASHMAIN'
#!/bin/bash

## VIASH START
# The following code has been auto-generated by Viash.
$( if [ ! -z ${VIASH_PAR_INPUT+x} ]; then echo "${VIASH_PAR_INPUT}" | sed "s#'#'\"'\"'#g;s#.*#par_input='&'#" ; else echo "# par_input="; fi )
$( if [ ! -z ${VIASH_PAR_INPUT_BAI+x} ]; then echo "${VIASH_PAR_INPUT_BAI}" | sed "s#'#'\"'\"'#g;s#.*#par_input_bai='&'#" ; else echo "# par_input_bai="; fi )
$( if [ ! -z ${VIASH_PAR_REF+x} ]; then echo "${VIASH_PAR_REF}" | sed "s#'#'\"'\"'#g;s#.*#par_ref='&'#" ; else echo "# par_ref="; fi )
$( if [ ! -z ${VIASH_PAR_OUT+x} ]; then echo "${VIASH_PAR_OUT}" | sed "s#'#'\"'\"'#g;s#.*#par_out='&'#" ; else echo "# par_out="; fi )
$( if [ ! -z ${VIASH_PAR_REGION+x} ]; then echo "${VIASH_PAR_REGION}" | sed "s#'#'\"'\"'#g;s#.*#par_region='&'#" ; else echo "# par_region="; fi )
$( if [ ! -z ${VIASH_PAR_BED+x} ]; then echo "${VIASH_PAR_BED}" | sed "s#'#'\"'\"'#g;s#.*#par_bed='&'#" ; else echo "# par_bed="; fi )
$( if [ ! -z ${VIASH_PAR_MIN_BQ+x} ]; then echo "${VIASH_PAR_MIN_BQ}" | sed "s#'#'\"'\"'#g;s#.*#par_min_bq='&'#" ; else echo "# par_min_bq="; fi )
$( if [ ! -z ${VIASH_PAR_MIN_ALT_BQ+x} ]; then echo "${VIASH_PAR_MIN_ALT_BQ}" | sed "s#'#'\"'\"'#g;s#.*#par_min_alt_bq='&'#" ; else echo "# par_min_alt_bq="; fi )
$( if [ ! -z ${VIASH_PAR_DEF_ALT_BQ+x} ]; then echo "${VIASH_PAR_DEF_ALT_BQ}" | sed "s#'#'\"'\"'#g;s#.*#par_def_alt_bq='&'#" ; else echo "# par_def_alt_bq="; fi )
$( if [ ! -z ${VIASH_PAR_MIN_JQ+x} ]; then echo "${VIASH_PAR_MIN_JQ}" | sed "s#'#'\"'\"'#g;s#.*#par_min_jq='&'#" ; else echo "# par_min_jq="; fi )
$( if [ ! -z ${VIASH_PAR_MIN_ALT_JQ+x} ]; then echo "${VIASH_PAR_MIN_ALT_JQ}" | sed "s#'#'\"'\"'#g;s#.*#par_min_alt_jq='&'#" ; else echo "# par_min_alt_jq="; fi )
$( if [ ! -z ${VIASH_PAR_DEF_ALT_JQ+x} ]; then echo "${VIASH_PAR_DEF_ALT_JQ}" | sed "s#'#'\"'\"'#g;s#.*#par_def_alt_jq='&'#" ; else echo "# par_def_alt_jq="; fi )
$( if [ ! -z ${VIASH_PAR_NO_BAQ+x} ]; then echo "${VIASH_PAR_NO_BAQ}" | sed "s#'#'\"'\"'#g;s#.*#par_no_baq='&'#" ; else echo "# par_no_baq="; fi )
$( if [ ! -z ${VIASH_PAR_NO_IDAQ+x} ]; then echo "${VIASH_PAR_NO_IDAQ}" | sed "s#'#'\"'\"'#g;s#.*#par_no_idaq='&'#" ; else echo "# par_no_idaq="; fi )
$( if [ ! -z ${VIASH_PAR_DEL_BAQ+x} ]; then echo "${VIASH_PAR_DEL_BAQ}" | sed "s#'#'\"'\"'#g;s#.*#par_del_baq='&'#" ; else echo "# par_del_baq="; fi )
$( if [ ! -z ${VIASH_PAR_NO_EXT_BAQ+x} ]; then echo "${VIASH_PAR_NO_EXT_BAQ}" | sed "s#'#'\"'\"'#g;s#.*#par_no_ext_baq='&'#" ; else echo "# par_no_ext_baq="; fi )
$( if [ ! -z ${VIASH_PAR_MIN_MQ+x} ]; then echo "${VIASH_PAR_MIN_MQ}" | sed "s#'#'\"'\"'#g;s#.*#par_min_mq='&'#" ; else echo "# par_min_mq="; fi )
$( if [ ! -z ${VIASH_PAR_MAX_MQ+x} ]; then echo "${VIASH_PAR_MAX_MQ}" | sed "s#'#'\"'\"'#g;s#.*#par_max_mq='&'#" ; else echo "# par_max_mq="; fi )
$( if [ ! -z ${VIASH_PAR_NO_MQ+x} ]; then echo "${VIASH_PAR_NO_MQ}" | sed "s#'#'\"'\"'#g;s#.*#par_no_mq='&'#" ; else echo "# par_no_mq="; fi )
$( if [ ! -z ${VIASH_PAR_CALL_INDELS+x} ]; then echo "${VIASH_PAR_CALL_INDELS}" | sed "s#'#'\"'\"'#g;s#.*#par_call_indels='&'#" ; else echo "# par_call_indels="; fi )
$( if [ ! -z ${VIASH_PAR_ONLY_INDELS+x} ]; then echo "${VIASH_PAR_ONLY_INDELS}" | sed "s#'#'\"'\"'#g;s#.*#par_only_indels='&'#" ; else echo "# par_only_indels="; fi )
$( if [ ! -z ${VIASH_PAR_SRC_QUAL+x} ]; then echo "${VIASH_PAR_SRC_QUAL}" | sed "s#'#'\"'\"'#g;s#.*#par_src_qual='&'#" ; else echo "# par_src_qual="; fi )
$( if [ ! -z ${VIASH_PAR_IGN_VCF+x} ]; then echo "${VIASH_PAR_IGN_VCF}" | sed "s#'#'\"'\"'#g;s#.*#par_ign_vcf='&'#" ; else echo "# par_ign_vcf="; fi )
$( if [ ! -z ${VIASH_PAR_DEF_NM_Q+x} ]; then echo "${VIASH_PAR_DEF_NM_Q}" | sed "s#'#'\"'\"'#g;s#.*#par_def_nm_q='&'#" ; else echo "# par_def_nm_q="; fi )
$( if [ ! -z ${VIASH_PAR_SIG+x} ]; then echo "${VIASH_PAR_SIG}" | sed "s#'#'\"'\"'#g;s#.*#par_sig='&'#" ; else echo "# par_sig="; fi )
$( if [ ! -z ${VIASH_PAR_BONF+x} ]; then echo "${VIASH_PAR_BONF}" | sed "s#'#'\"'\"'#g;s#.*#par_bonf='&'#" ; else echo "# par_bonf="; fi )
$( if [ ! -z ${VIASH_PAR_MIN_COV+x} ]; then echo "${VIASH_PAR_MIN_COV}" | sed "s#'#'\"'\"'#g;s#.*#par_min_cov='&'#" ; else echo "# par_min_cov="; fi )
$( if [ ! -z ${VIASH_PAR_MAX_DEPTH+x} ]; then echo "${VIASH_PAR_MAX_DEPTH}" | sed "s#'#'\"'\"'#g;s#.*#par_max_depth='&'#" ; else echo "# par_max_depth="; fi )
$( if [ ! -z ${VIASH_PAR_ILLUMINA_13+x} ]; then echo "${VIASH_PAR_ILLUMINA_13}" | sed "s#'#'\"'\"'#g;s#.*#par_illumina_13='&'#" ; else echo "# par_illumina_13="; fi )
$( if [ ! -z ${VIASH_PAR_USE_ORPHAN+x} ]; then echo "${VIASH_PAR_USE_ORPHAN}" | sed "s#'#'\"'\"'#g;s#.*#par_use_orphan='&'#" ; else echo "# par_use_orphan="; fi )
$( if [ ! -z ${VIASH_PAR_PLP_SUMMARY_ONLY+x} ]; then echo "${VIASH_PAR_PLP_SUMMARY_ONLY}" | sed "s#'#'\"'\"'#g;s#.*#par_plp_summary_only='&'#" ; else echo "# par_plp_summary_only="; fi )
$( if [ ! -z ${VIASH_PAR_NO_DEFAULT_FILTER+x} ]; then echo "${VIASH_PAR_NO_DEFAULT_FILTER}" | sed "s#'#'\"'\"'#g;s#.*#par_no_default_filter='&'#" ; else echo "# par_no_default_filter="; fi )
$( if [ ! -z ${VIASH_PAR_FORCE_OVERWRITE+x} ]; then echo "${VIASH_PAR_FORCE_OVERWRITE}" | sed "s#'#'\"'\"'#g;s#.*#par_force_overwrite='&'#" ; else echo "# par_force_overwrite="; fi )
$( if [ ! -z ${VIASH_PAR_VERBOSE+x} ]; then echo "${VIASH_PAR_VERBOSE}" | sed "s#'#'\"'\"'#g;s#.*#par_verbose='&'#" ; else echo "# par_verbose="; fi )
$( if [ ! -z ${VIASH_PAR_DEBUG+x} ]; then echo "${VIASH_PAR_DEBUG}" | sed "s#'#'\"'\"'#g;s#.*#par_debug='&'#" ; else echo "# par_debug="; fi )
$( if [ ! -z ${VIASH_META_NAME+x} ]; then echo "${VIASH_META_NAME}" | sed "s#'#'\"'\"'#g;s#.*#meta_name='&'#" ; else echo "# meta_name="; fi )
$( if [ ! -z ${VIASH_META_FUNCTIONALITY_NAME+x} ]; then echo "${VIASH_META_FUNCTIONALITY_NAME}" | sed "s#'#'\"'\"'#g;s#.*#meta_functionality_name='&'#" ; else echo "# meta_functionality_name="; fi )
$( if [ ! -z ${VIASH_META_RESOURCES_DIR+x} ]; then echo "${VIASH_META_RESOURCES_DIR}" | sed "s#'#'\"'\"'#g;s#.*#meta_resources_dir='&'#" ; else echo "# meta_resources_dir="; fi )
$( if [ ! -z ${VIASH_META_EXECUTABLE+x} ]; then echo "${VIASH_META_EXECUTABLE}" | sed "s#'#'\"'\"'#g;s#.*#meta_executable='&'#" ; else echo "# meta_executable="; fi )
$( if [ ! -z ${VIASH_META_CONFIG+x} ]; then echo "${VIASH_META_CONFIG}" | sed "s#'#'\"'\"'#g;s#.*#meta_config='&'#" ; else echo "# meta_config="; fi )
$( if [ ! -z ${VIASH_META_TEMP_DIR+x} ]; then echo "${VIASH_META_TEMP_DIR}" | sed "s#'#'\"'\"'#g;s#.*#meta_temp_dir='&'#" ; else echo "# meta_temp_dir="; fi )
$( if [ ! -z ${VIASH_META_CPUS+x} ]; then echo "${VIASH_META_CPUS}" | sed "s#'#'\"'\"'#g;s#.*#meta_cpus='&'#" ; else echo "# meta_cpus="; fi )
$( if [ ! -z ${VIASH_META_MEMORY_B+x} ]; then echo "${VIASH_META_MEMORY_B}" | sed "s#'#'\"'\"'#g;s#.*#meta_memory_b='&'#" ; else echo "# meta_memory_b="; fi )
$( if [ ! -z ${VIASH_META_MEMORY_KB+x} ]; then echo "${VIASH_META_MEMORY_KB}" | sed "s#'#'\"'\"'#g;s#.*#meta_memory_kb='&'#" ; else echo "# meta_memory_kb="; fi )
$( if [ ! -z ${VIASH_META_MEMORY_MB+x} ]; then echo "${VIASH_META_MEMORY_MB}" | sed "s#'#'\"'\"'#g;s#.*#meta_memory_mb='&'#" ; else echo "# meta_memory_mb="; fi )
$( if [ ! -z ${VIASH_META_MEMORY_GB+x} ]; then echo "${VIASH_META_MEMORY_GB}" | sed "s#'#'\"'\"'#g;s#.*#meta_memory_gb='&'#" ; else echo "# meta_memory_gb="; fi )
$( if [ ! -z ${VIASH_META_MEMORY_TB+x} ]; then echo "${VIASH_META_MEMORY_TB}" | sed "s#'#'\"'\"'#g;s#.*#meta_memory_tb='&'#" ; else echo "# meta_memory_tb="; fi )
$( if [ ! -z ${VIASH_META_MEMORY_PB+x} ]; then echo "${VIASH_META_MEMORY_PB}" | sed "s#'#'\"'\"'#g;s#.*#meta_memory_pb='&'#" ; else echo "# meta_memory_pb="; fi )
$( if [ ! -z ${VIASH_META_MEMORY_KIB+x} ]; then echo "${VIASH_META_MEMORY_KIB}" | sed "s#'#'\"'\"'#g;s#.*#meta_memory_kib='&'#" ; else echo "# meta_memory_kib="; fi )
$( if [ ! -z ${VIASH_META_MEMORY_MIB+x} ]; then echo "${VIASH_META_MEMORY_MIB}" | sed "s#'#'\"'\"'#g;s#.*#meta_memory_mib='&'#" ; else echo "# meta_memory_mib="; fi )
$( if [ ! -z ${VIASH_META_MEMORY_GIB+x} ]; then echo "${VIASH_META_MEMORY_GIB}" | sed "s#'#'\"'\"'#g;s#.*#meta_memory_gib='&'#" ; else echo "# meta_memory_gib="; fi )
$( if [ ! -z ${VIASH_META_MEMORY_TIB+x} ]; then echo "${VIASH_META_MEMORY_TIB}" | sed "s#'#'\"'\"'#g;s#.*#meta_memory_tib='&'#" ; else echo "# meta_memory_tib="; fi )
$( if [ ! -z ${VIASH_META_MEMORY_PIB+x} ]; then echo "${VIASH_META_MEMORY_PIB}" | sed "s#'#'\"'\"'#g;s#.*#meta_memory_pib='&'#" ; else echo "# meta_memory_pib="; fi )

## VIASH END

# Unset all parameters that are set to "false"
unset_if_false=(
    par_no_baq
    par_no_idaq
    par_del_baq
    par_no_ext_baq
    par_no_mq
    par_call_indels
    par_only_indels
    par_src_qual
    par_illumina_13
    par_use_orphan
    par_plp_summary_only
    par_no_default_filter
    par_force_overwrite
    par_verbose
    par_debug
)

for par in \${unset_if_false[@]}; do
    test_val="\${!par}"
    [[ "\$test_val" == "false" ]] && unset \$par
done

# Run lofreq call
lofreq call \\
  -f "\$par_ref" \\
  -o "\$par_out" \\
  \${par_region:+-r "\${par_region}"} \\
  \${par_bed:+-l "\${par_bed}"} \\
  \${par_min_bq:+-q "\${par_min_bq}"} \\
  \${par_min_alt_bq:+-Q "\${par_min_alt_bq}"} \\
  \${par_def_alt_bq:+-R "\${par_def_alt_bq}"} \\
  \${par_min_jq:+-j "\${par_min_jq}"} \\
  \${par_alt_jq:+-K "\${par_alt_jq}"} \\
  \${par_no_baq:+-B} \\
  \${par_no_idaq:+-A} \\
  \${par_del_baq:+-D} \\
  \${par_no_ext_baq:+-e} \\
  \${par_min_mq:+-m "\${par_min_mq}"} \\
  \${par_max_mq:+-M "\${par_max_mq}"} \\
  \${par_no_mq:+-N} \\
  \${par_call_indels:+--call-indels} \\
  \${par_only_indels:+--only-indels} \\
  \${par_src_qual:+-s} \\
  \${par_ign_vcf:+-S "\${par_ign_vcf}"} \\
  \${par_def_nm_q:+-T "\${par_def_nm_q}"} \\
  \${par_sig:+-a "\${par_sig}"} \\
  \${par_bonf:+-b "\${par_bonf}"} \\
  \${par_min_cov:+-C "\${par_min_cov}"} \\
  \${par_max_depth:+-d "\${par_max_depth}"} \\
  \${par_illumina_13:+--illumina-1.3} \\
  \${par_use_orphan:+--use-orphan} \\
  \${par_plp_summary_only:+--plp-summary-only} \\
  \${par_no_default_filter:+--no-default-filter} \\
  \${par_force_overwrite:+--force-overwrite} \\
  \${par_verbose:+--verbose} \\
  \${par_debug:+--debug} \\
  "\$par_input"
VIASHMAIN
bash "\$tempscript" &
wait "\$!"

VIASHEOF


if [[ "$VIASH_ENGINE_TYPE" == "docker" ]]; then
  # strip viash automount from file paths
  
  if [ ! -z "$VIASH_PAR_INPUT" ]; then
    VIASH_PAR_INPUT=$(ViashDockerStripAutomount "$VIASH_PAR_INPUT")
  fi
  if [ ! -z "$VIASH_PAR_INPUT_BAI" ]; then
    VIASH_PAR_INPUT_BAI=$(ViashDockerStripAutomount "$VIASH_PAR_INPUT_BAI")
  fi
  if [ ! -z "$VIASH_PAR_REF" ]; then
    VIASH_PAR_REF=$(ViashDockerStripAutomount "$VIASH_PAR_REF")
  fi
  if [ ! -z "$VIASH_PAR_OUT" ]; then
    VIASH_PAR_OUT=$(ViashDockerStripAutomount "$VIASH_PAR_OUT")
  fi
  if [ ! -z "$VIASH_PAR_BED" ]; then
    VIASH_PAR_BED=$(ViashDockerStripAutomount "$VIASH_PAR_BED")
  fi
  if [ ! -z "$VIASH_PAR_IGN_VCF" ]; then
    VIASH_PAR_IGN_VCF=$(ViashDockerStripAutomount "$VIASH_PAR_IGN_VCF")
  fi
  if [ ! -z "$VIASH_META_RESOURCES_DIR" ]; then
    VIASH_META_RESOURCES_DIR=$(ViashDockerStripAutomount "$VIASH_META_RESOURCES_DIR")
  fi
  if [ ! -z "$VIASH_META_EXECUTABLE" ]; then
    VIASH_META_EXECUTABLE=$(ViashDockerStripAutomount "$VIASH_META_EXECUTABLE")
  fi
  if [ ! -z "$VIASH_META_CONFIG" ]; then
    VIASH_META_CONFIG=$(ViashDockerStripAutomount "$VIASH_META_CONFIG")
  fi
  if [ ! -z "$VIASH_META_TEMP_DIR" ]; then
    VIASH_META_TEMP_DIR=$(ViashDockerStripAutomount "$VIASH_META_TEMP_DIR")
  fi
fi


# check whether required files exist
if [ ! -z "$VIASH_PAR_OUT" ] && [ ! -e "$VIASH_PAR_OUT" ]; then
  ViashError "Output file '$VIASH_PAR_OUT' does not exist."
  exit 1
fi


exit 0