{ "$schema": "https://json-schema.org/draft/2020-12/schema", "title": "bcl_convert", "description": "Convert bcl files to fastq files using bcl-convert.\nInformation about upgrading from bcl2fastq via\n[Upgrading from bcl2fastq to BCL Convert](https://emea.support.illumina.com/bulletins/2020/10/upgrading-from-bcl2fastq-to-bcl-convert.html)\nand [BCL Convert Compatible Products](https://support.illumina.com/sequencing/sequencing_software/bcl-convert/compatibility.html)\n", "type": "object", "$defs": { "input arguments": { "title": "Input arguments", "type": "object", "description": "No description", "properties": { "bcl_input_directory": { "type": "string", "format": "path", "exists": true, "description": "Input run directory", "help_text": "Type: `file`, multiple: `False`, required, direction: `input`, example: `\"bcl_dir\"`. " }, "sample_sheet": { "type": "string", "format": "path", "description": "Path to SampleSheet.csv file (default searched for in --bcl_input_directory)", "help_text": "Type: `file`, multiple: `False`, direction: `input`, example: `\"bcl_dir/sample_sheet.csv\"`. " }, "run_info": { "type": "string", "format": "path", "description": "Path to RunInfo.xml file (default root of BCL input directory)", "help_text": "Type: `file`, multiple: `False`, direction: `input`, example: `\"bcl_dir/RunInfo.xml\"`. " } } }, "lane and tile settings": { "title": "Lane and tile settings", "type": "object", "description": "No description", "properties": { "bcl_only_lane": { "type": "integer", "description": "Convert only specified lane number (default all lanes)", "help_text": "Type: `integer`, multiple: `False`, example: `1`. " }, "first_tile_only": { "type": "boolean", "description": "Only convert first tile of input (for testing & debugging)", "help_text": "Type: `boolean`, multiple: `False`, example: `true`. " }, "tiles": { "type": "string", "description": "Process only a subset of tiles by a regular expression", "help_text": "Type: `string`, multiple: `False`, example: `\"s_[0-9]+_1\"`. " }, "exclude_tiles": { "type": "string", "description": "Exclude set of tiles by a regular expression", "help_text": "Type: `string`, multiple: `False`, example: `\"s_[0-9]+_1\"`. " } } }, "resource arguments": { "title": "Resource arguments", "type": "object", "description": "No description", "properties": { "shared_thread_odirect_output": { "type": "boolean", "description": "Use linux native asynchronous io (io_submit) for file output (Default=false)", "help_text": "Type: `boolean`, multiple: `False`, example: `true`. " }, "bcl_num_parallel_tiles": { "type": "integer", "description": "\\# of tiles to process in parallel (default 1)", "help_text": "Type: `integer`, multiple: `False`, example: `1`. " }, "bcl_num_conversion_threads": { "type": "integer", "description": "\\# of threads for conversion (per tile, default # cpu threads)", "help_text": "Type: `integer`, multiple: `False`, example: `1`. " }, "bcl_num_compression_threads": { "type": "integer", "description": "\\# of threads for fastq.gz output compression (per tile, default # cpu threads, or HW+12)", "help_text": "Type: `integer`, multiple: `False`, example: `1`. " }, "bcl_num_decompression_threads": { "type": "integer", "description": "\\# of threads for bcl/cbcl input decompression (per tile, default half # cpu threads, or HW+8)", "help_text": "Type: `integer`, multiple: `False`, example: `1`. " } } }, "run arguments": { "title": "Run arguments", "type": "object", "description": "No description", "properties": { "bcl_only_matched_reads": { "type": "boolean", "description": "For pure BCL conversion, do not output files for 'Undetermined' [unmatched] reads (output by default)", "help_text": "Type: `boolean`, multiple: `False`, example: `true`. " }, "no_lane_splitting": { "type": "boolean", "description": "Do not split FASTQ file by lane (false by default)", "help_text": "Type: `boolean`, multiple: `False`, example: `true`. " }, "num_unknown_barcodes_reported": { "type": "integer", "description": "\\# of Top Unknown Barcodes to output (1000 by default)", "help_text": "Type: `integer`, multiple: `False`, example: `1000`. " }, "bcl_validate_sample_sheet_only": { "type": "boolean", "description": "Only validate RunInfo.xml & SampleSheet files (produce no FASTQ files)", "help_text": "Type: `boolean`, multiple: `False`, example: `true`. " }, "strict_mode": { "type": "boolean", "description": "Abort if any files are missing (false by default)", "help_text": "Type: `boolean`, multiple: `False`, example: `true`. " }, "sample_name_column_enabled": { "type": "boolean", "description": "Use sample sheet 'Sample_Name' column when naming fastq files & subdirectories", "help_text": "Type: `boolean`, multiple: `False`, example: `true`. " } } }, "output arguments": { "title": "Output arguments", "type": "object", "description": "No description", "properties": { "output_directory": { "type": "string", "format": "path", "description": "Output directory containig fastq files", "help_text": "Type: `file`, multiple: `False`, required, default: `\"$id.$key.output_directory\"`, direction: `output`, example: `\"fastq_dir\"`. ", "default": "$id.$key.output_directory" }, "bcl_sampleproject_subdirectories": { "type": "boolean", "description": "Output to subdirectories based upon sample sheet 'Sample_Project' column", "help_text": "Type: `boolean`, multiple: `False`, example: `true`. " }, "fastq_gzip_compression_level": { "type": "integer", "description": "Set fastq output compression level 0-9 (default 1)", "help_text": "Type: `integer`, multiple: `False`, example: `1`. " }, "reports": { "type": "string", "format": "path", "description": "Reports directory", "help_text": "Type: `file`, multiple: `False`, default: `\"$id.$key.reports\"`, direction: `output`, example: `\"reports_dir\"`. ", "default": "$id.$key.reports" }, "logs": { "type": "string", "format": "path", "description": "Reports directory", "help_text": "Type: `file`, multiple: `False`, default: `\"$id.$key.logs\"`, direction: `output`, example: `\"logs_dir\"`. ", "default": "$id.$key.logs" }, "force": { "type": "boolean", "description": "Allow destination directory to already exist and overwrite files.\n", "help_text": "Type: `boolean`, multiple: `False`, example: `true`. " } } }, "nextflow input-output arguments": { "title": "Nextflow input-output arguments", "type": "object", "description": "Input/output parameters for Nextflow itself. Please note that both publishDir and publish_dir are supported but at least one has to be configured.", "properties": { "publish_dir": { "type": "string", "description": "Path to an output directory.", "help_text": "Type: `string`, multiple: `False`, required, example: `\"output/\"`. " } } } }, "allOf": [ { "$ref": "#/$defs/input arguments" }, { "$ref": "#/$defs/lane and tile settings" }, { "$ref": "#/$defs/resource arguments" }, { "$ref": "#/$defs/run arguments" }, { "$ref": "#/$defs/output arguments" }, { "$ref": "#/$defs/nextflow input-output arguments" } ] }