name: "bcl_convert" version: "main" authors: - name: "Toni Verbeiren" roles: - "author" - "maintainer" info: links: github: "tverbeiren" linkedin: "verbeiren" organizations: - name: "Data Intuitive" href: "https://www.data-intuitive.com" role: "Data Scientist and CEO" - name: "Dorien Roosen" roles: - "author" info: links: email: "dorien@data-intuitive.com" github: "dorien-er" linkedin: "dorien-roosen" organizations: - name: "Data Intuitive" href: "https://www.data-intuitive.com" role: "Data Scientist" argument_groups: - name: "Input arguments" arguments: - type: "file" name: "--bcl_input_directory" alternatives: - "-i" description: "Input run directory" info: null example: - "bcl_dir" must_exist: true create_parent: true required: true direction: "input" multiple: false multiple_sep: ";" - type: "file" name: "--sample_sheet" alternatives: - "-s" description: "Path to SampleSheet.csv file (default searched for in --bcl_input_directory)" info: null example: - "bcl_dir/sample_sheet.csv" must_exist: true create_parent: true required: false direction: "input" multiple: false multiple_sep: ";" - type: "file" name: "--run_info" description: "Path to RunInfo.xml file (default root of BCL input directory)" info: null example: - "bcl_dir/RunInfo.xml" must_exist: true create_parent: true required: false direction: "input" multiple: false multiple_sep: ";" - name: "Lane and tile settings" arguments: - type: "integer" name: "--bcl_only_lane" description: "Convert only specified lane number (default all lanes)" info: null example: - 1 required: false direction: "input" multiple: false multiple_sep: ";" - type: "boolean" name: "--first_tile_only" description: "Only convert first tile of input (for testing & debugging)" info: null example: - true required: false direction: "input" multiple: false multiple_sep: ";" - type: "string" name: "--tiles" description: "Process only a subset of tiles by a regular expression" info: null example: - "s_[0-9]+_1" required: false direction: "input" multiple: false multiple_sep: ";" - type: "string" name: "--exclude_tiles" description: "Exclude set of tiles by a regular expression" info: null example: - "s_[0-9]+_1" required: false direction: "input" multiple: false multiple_sep: ";" - name: "Resource arguments" arguments: - type: "boolean" name: "--shared_thread_odirect_output" description: "Use linux native asynchronous io (io_submit) for file output (Default=false)" info: null example: - true required: false direction: "input" multiple: false multiple_sep: ";" - type: "integer" name: "--bcl_num_parallel_tiles" description: "\\# of tiles to process in parallel (default 1)" info: null example: - 1 required: false direction: "input" multiple: false multiple_sep: ";" - type: "integer" name: "--bcl_num_conversion_threads" description: "\\# of threads for conversion (per tile, default # cpu threads)" info: null example: - 1 required: false direction: "input" multiple: false multiple_sep: ";" - type: "integer" name: "--bcl_num_compression_threads" description: "\\# of threads for fastq.gz output compression (per tile, default\ \ # cpu threads, or HW+12)" info: null example: - 1 required: false direction: "input" multiple: false multiple_sep: ";" - type: "integer" name: "--bcl_num_decompression_threads" description: "\\# of threads for bcl/cbcl input decompression (per tile, default\ \ half # cpu threads, or HW+8). Only applies when preloading files" info: null example: - 1 required: false direction: "input" multiple: false multiple_sep: ";" - name: "Run arguments" arguments: - type: "boolean" name: "--bcl_only_matched_reads" description: "For pure BCL conversion, do not output files for 'Undetermined'\ \ [unmatched] reads (output by default)" info: null example: - true required: false direction: "input" multiple: false multiple_sep: ";" - type: "boolean" name: "--no_lane_splitting" description: "Do not split FASTQ file by lane (false by default)" info: null example: - true required: false direction: "input" multiple: false multiple_sep: ";" - type: "integer" name: "--num_unknown_barcodes_reported" description: "\\# of Top Unknown Barcodes to output (1000 by default)" info: null example: - 1000 required: false direction: "input" multiple: false multiple_sep: ";" - type: "boolean" name: "--bcl_validate_sample_sheet_only" description: "Only validate RunInfo.xml & SampleSheet files (produce no FASTQ\ \ files)" info: null example: - true required: false direction: "input" multiple: false multiple_sep: ";" - type: "boolean" name: "--strict_mode" description: "Abort if any files are missing (false by default)" info: null example: - true required: false direction: "input" multiple: false multiple_sep: ";" - type: "boolean" name: "--sample_name_column_enabled" description: "Use sample sheet 'Sample_Name' column when naming fastq files &\ \ subdirectories" info: null example: - true required: false direction: "input" multiple: false multiple_sep: ";" - name: "Output arguments" arguments: - type: "file" name: "--output_directory" alternatives: - "-o" description: "Output directory containig fastq files" info: null example: - "fastq_dir" must_exist: true create_parent: true required: true direction: "output" multiple: false multiple_sep: ";" - type: "boolean" name: "--bcl_sampleproject_subdirectories" description: "Output to subdirectories based upon sample sheet 'Sample_Project'\ \ column" info: null example: - true required: false direction: "input" multiple: false multiple_sep: ";" - type: "integer" name: "--fastq_gzip_compression_level" description: "Set fastq output compression level 0-9 (default 1)" info: null example: - 1 required: false direction: "input" multiple: false multiple_sep: ";" - type: "file" name: "--reports" description: "Reports directory" info: null example: - "reports_dir" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" - type: "file" name: "--logs" description: "Reports directory" info: null example: - "logs_dir" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" resources: - type: "bash_script" path: "script.sh" is_executable: true description: "Convert bcl files to fastq files using bcl-convert.\nInformation about\ \ upgrading from bcl2fastq via\n[Upgrading from bcl2fastq to BCL Convert](https://emea.support.illumina.com/bulletins/2020/10/upgrading-from-bcl2fastq-to-bcl-convert.html)\n\ and [BCL Convert Compatible Products](https://support.illumina.com/sequencing/sequencing_software/bcl-convert/compatibility.html)\n" test_resources: - type: "bash_script" path: "test.sh" is_executable: true info: null status: "enabled" requirements: commands: - "ps" keywords: - "demultiplex" - "fastq" - "bcl" - "illumina" license: "Proprietary" links: repository: "https://github.com/viash-hub/biobox" homepage: "https://support.illumina.com/sequencing/sequencing_software/bcl-convert.html" documentation: "https://support.illumina.com/downloads/bcl-convert-user-guide.html" runners: - type: "executable" id: "executable" docker_setup_strategy: "ifneedbepullelsecachedbuild" - type: "nextflow" id: "nextflow" directives: tag: "$id" auto: simplifyInput: true simplifyOutput: false transcript: false publish: false config: labels: mem1gb: "memory = 1000000000.B" mem2gb: "memory = 2000000000.B" mem5gb: "memory = 5000000000.B" mem10gb: "memory = 10000000000.B" mem20gb: "memory = 20000000000.B" mem50gb: "memory = 50000000000.B" mem100gb: "memory = 100000000000.B" mem200gb: "memory = 200000000000.B" mem500gb: "memory = 500000000000.B" mem1tb: "memory = 1000000000000.B" mem2tb: "memory = 2000000000000.B" mem5tb: "memory = 5000000000000.B" mem10tb: "memory = 10000000000000.B" mem20tb: "memory = 20000000000000.B" mem50tb: "memory = 50000000000000.B" mem100tb: "memory = 100000000000000.B" mem200tb: "memory = 200000000000000.B" mem500tb: "memory = 500000000000000.B" mem1gib: "memory = 1073741824.B" mem2gib: "memory = 2147483648.B" mem4gib: "memory = 4294967296.B" mem8gib: "memory = 8589934592.B" mem16gib: "memory = 17179869184.B" mem32gib: "memory = 34359738368.B" mem64gib: "memory = 68719476736.B" mem128gib: "memory = 137438953472.B" mem256gib: "memory = 274877906944.B" mem512gib: "memory = 549755813888.B" mem1tib: "memory = 1099511627776.B" mem2tib: "memory = 2199023255552.B" mem4tib: "memory = 4398046511104.B" mem8tib: "memory = 8796093022208.B" mem16tib: "memory = 17592186044416.B" mem32tib: "memory = 35184372088832.B" mem64tib: "memory = 70368744177664.B" mem128tib: "memory = 140737488355328.B" mem256tib: "memory = 281474976710656.B" mem512tib: "memory = 562949953421312.B" cpu1: "cpus = 1" cpu2: "cpus = 2" cpu5: "cpus = 5" cpu10: "cpus = 10" cpu20: "cpus = 20" cpu50: "cpus = 50" cpu100: "cpus = 100" cpu200: "cpus = 200" cpu500: "cpus = 500" cpu1000: "cpus = 1000" debug: false container: "docker" engines: - type: "docker" id: "docker" image: "debian:trixie-slim" target_registry: "images.viash-hub.com" target_tag: "main" namespace_separator: "/" setup: - type: "apt" packages: - "wget" - "gdb" - "which" - "hostname" - "alien" - "procps" interactive: false - type: "docker" run: - "wget https://s3.amazonaws.com/webdata.illumina.com/downloads/software/bcl-convert/bcl-convert-4.2.7-2.el8.x86_64.rpm\ \ -O /tmp/bcl-convert.rpm && \\\nalien -i /tmp/bcl-convert.rpm && \\\nrm -rf\ \ /var/lib/apt/lists/* && \\\nrm /tmp/bcl-convert.rpm\n" - type: "docker" run: - "echo \"bcl-convert: \\\"$(bcl-convert -V 2>&1 >/dev/null | sed -n '/Version/\ \ s/^bcl-convert\\ Version //p')\\\"\" > /var/software_versions.txt\n" entrypoint: [] cmd: null - type: "native" id: "native" build_info: config: "src/bcl_convert/config.vsh.yaml" runner: "executable" engine: "docker|native" output: "target/executable/bcl_convert" executable: "target/executable/bcl_convert/bcl_convert" viash_version: "0.9.0" git_commit: "7f8bcc2b3e1ffaac9778b6acb42420b19660d1a1" git_remote: "https://x-access-token:ghs_aSDBedV4vU66pddFDN6d8UEy0ZQApn08RAsh@github.com/viash-hub/biobox" git_tag: "v0.2.0-3-g7f8bcc2" package_config: name: "biobox" version: "main" description: "A collection of bioinformatics tools for working with sequence data.\n" info: null viash_version: "0.9.0" source: "src" target: "target" config_mods: - ".requirements.commands := ['ps']\n" - ".engines += { type: \"native\" }" - ".engines[.type == 'docker'].target_registry := 'images.viash-hub.com'" - ".engines[.type == 'docker'].target_tag := 'main'" keywords: - "bioinformatics" - "modules" - "sequencing" license: "MIT" organization: "vsh" links: repository: "https://github.com/viash-hub/biobox" issue_tracker: "https://github.com/viash-hub/biobox/issues"