name: "bbmap_bbsplit" namespace: "bbmap" version: "main" argument_groups: - name: "Input" arguments: - type: "string" name: "--id" description: "Sample ID" info: null required: false direction: "input" multiple: false multiple_sep: ";" - type: "boolean_true" name: "--paired" description: "Paired fastq files or not?" info: null direction: "input" - type: "file" name: "--input" description: "Input fastq files, either one or two (paired), separated by \";\"\ ." info: null example: - "reads.fastq" must_exist: true create_parent: true required: false direction: "input" multiple: true multiple_sep: ";" - type: "file" name: "--ref" description: "Reference FASTA files, separated by \";\". The primary reference\ \ should be specified first." info: null must_exist: true create_parent: true required: false direction: "input" multiple: true multiple_sep: ";" - type: "boolean_true" name: "--only_build_index" description: "If set, only builds the index. Otherwise, mapping is performed." info: null direction: "input" - type: "file" name: "--build" description: "Index to be used for mapping. \n" info: null must_exist: true create_parent: true required: false direction: "input" multiple: false multiple_sep: ";" - type: "string" name: "--qin" description: "Set to 33 or 64 to specify input quality value ASCII offset. Automatically\ \ detected if\nnot specified.\n" info: null required: false direction: "input" multiple: false multiple_sep: ";" - type: "boolean_true" name: "--interleaved" description: "True forces paired/interleaved input; false forces single-ended\ \ mapping.\nIf not specified, interleaved status will be autodetected from read\ \ names.\n" info: null direction: "input" - type: "integer" name: "--maxindel" description: "Don't look for indels longer than this. Lower is faster. Set to\ \ >=100k for RNA-seq.\n" info: null example: - 20 required: false direction: "input" multiple: false multiple_sep: ";" - type: "double" name: "--minratio" description: "Fraction of max alignment score required to keep a site. Higher\ \ is faster.\n" info: null example: - 0.56 required: false direction: "input" multiple: false multiple_sep: ";" - type: "integer" name: "--minhits" description: "Minimum number of seed hits required for candidate sites. Higher\ \ is faster.\n" info: null example: - 1 required: false direction: "input" multiple: false multiple_sep: ";" - type: "string" name: "--ambiguous" description: "Set behavior on ambiguously-mapped reads (with multiple top-scoring\ \ mapping locations).\n * best Use the first best site (Default)\n * toss\ \ Consider unmapped\n * random Select one top-scoring site randomly\n \ \ * all Retain all top-scoring sites. Does not work yet with SAM output\n" info: null example: - "best" required: false choices: - "best" - "toss" - "random" - "all" direction: "input" multiple: false multiple_sep: ";" - type: "string" name: "--ambiguous2" description: "Set behavior only for reads that map ambiguously to multiple different\ \ references.\nNormal 'ambiguous=' controls behavior on all ambiguous reads;\n\ Ambiguous2 excludes reads that map ambiguously within a single reference.\n\ \ * best Use the first best site (Default)\n * toss Consider unmapped\n\ \ * all Write a copy to the output for each reference to which it maps\n\ \ * split Write a copy to the AMBIGUOUS_ output for each reference to which\ \ it maps\n" info: null example: - "best" required: false choices: - "best" - "toss" - "all" - "split" direction: "input" multiple: false multiple_sep: ";" - type: "string" name: "--qtrim" description: "Quality-trim ends to Q5 before mapping. Options are 'l' (left),\ \ 'r' (right), and 'lr' (both).\n" info: null required: false choices: - "l" - "r" - "lr" direction: "input" multiple: false multiple_sep: ";" - type: "boolean_true" name: "--untrim" description: "Undo trimming after mapping. Untrimmed bases will be soft-clipped\ \ in cigar strings." info: null direction: "input" - name: "Output" arguments: - type: "file" name: "--index" description: "Location to write the index.\n" info: null example: - "BBSplit_index" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" - type: "file" name: "--fastq_1" description: "Output file for read 1.\n" info: null example: - "read_out1.fastq" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" - type: "file" name: "--fastq_2" description: "Output file for read 2.\n" info: null example: - "read_out2.fastq" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" - type: "file" name: "--sam2bam" alternatives: - "--bs" description: "Write a shell script to 'file' that will turn the sam output into\ \ a sorted, indexed bam file.\n" info: null example: - "script.sh" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" - type: "file" name: "--scafstats" description: "Write statistics on how many reads mapped to which scaffold to this\ \ file.\n" info: null example: - "scaffold_stats.txt" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" - type: "file" name: "--refstats" description: "Write statistics on how many reads were assigned to which reference\ \ to this file.\nUnmapped reads whose mate mapped to a reference are considered\ \ assigned and will be counted.\n" info: null example: - "reference_stats.txt" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" - type: "boolean_true" name: "--nzo" description: "Only print lines with nonzero coverage." info: null direction: "input" - type: "string" name: "--bbmap_args" description: "Additional arguments from BBMap to pass to BBSplit.\n" info: null required: false direction: "input" multiple: false multiple_sep: ";" resources: - type: "bash_script" path: "script.sh" is_executable: true description: "Split sequencing reads by mapping them to multiple references simultaneously." test_resources: - type: "bash_script" path: "test.sh" is_executable: true info: null status: "enabled" scope: image: "public" target: "public" requirements: commands: - "ps" license: "BBTools Copyright (c) 2014" links: repository: "https://github.com/BioInfoTools/BBMap/blob/master/sh/bbsplit.sh" homepage: "https://jgi.doe.gov/data-and-tools/software-tools/bbtools/bb-tools-user-guide/" documentation: "https://jgi.doe.gov/data-and-tools/software-tools/bbtools/bb-tools-user-guide/bbmap-guide/" runners: - type: "executable" id: "executable" docker_setup_strategy: "ifneedbepullelsecachedbuild" - type: "nextflow" id: "nextflow" directives: tag: "$id" auto: simplifyInput: true simplifyOutput: false transcript: false publish: false config: labels: mem1gb: "memory = 1000000000.B" mem2gb: "memory = 2000000000.B" mem5gb: "memory = 5000000000.B" mem10gb: "memory = 10000000000.B" mem20gb: "memory = 20000000000.B" mem50gb: "memory = 50000000000.B" mem100gb: "memory = 100000000000.B" mem200gb: "memory = 200000000000.B" mem500gb: "memory = 500000000000.B" mem1tb: "memory = 1000000000000.B" mem2tb: "memory = 2000000000000.B" mem5tb: "memory = 5000000000000.B" mem10tb: "memory = 10000000000000.B" mem20tb: "memory = 20000000000000.B" mem50tb: "memory = 50000000000000.B" mem100tb: "memory = 100000000000000.B" mem200tb: "memory = 200000000000000.B" mem500tb: "memory = 500000000000000.B" mem1gib: "memory = 1073741824.B" mem2gib: "memory = 2147483648.B" mem4gib: "memory = 4294967296.B" mem8gib: "memory = 8589934592.B" mem16gib: "memory = 17179869184.B" mem32gib: "memory = 34359738368.B" mem64gib: "memory = 68719476736.B" mem128gib: "memory = 137438953472.B" mem256gib: "memory = 274877906944.B" mem512gib: "memory = 549755813888.B" mem1tib: "memory = 1099511627776.B" mem2tib: "memory = 2199023255552.B" mem4tib: "memory = 4398046511104.B" mem8tib: "memory = 8796093022208.B" mem16tib: "memory = 17592186044416.B" mem32tib: "memory = 35184372088832.B" mem64tib: "memory = 70368744177664.B" mem128tib: "memory = 140737488355328.B" mem256tib: "memory = 281474976710656.B" mem512tib: "memory = 562949953421312.B" cpu1: "cpus = 1" cpu2: "cpus = 2" cpu5: "cpus = 5" cpu10: "cpus = 10" cpu20: "cpus = 20" cpu50: "cpus = 50" cpu100: "cpus = 100" cpu200: "cpus = 200" cpu500: "cpus = 500" cpu1000: "cpus = 1000" debug: false container: "docker" engines: - type: "docker" id: "docker" image: "ubuntu:22.04" target_registry: "images.viash-hub.com" target_tag: "main" namespace_separator: "/" setup: - type: "docker" run: - "apt-get update && \\\napt-get install -y build-essential openjdk-17-jdk wget\ \ tar && \\\nwget --no-check-certificate https://sourceforge.net/projects/bbmap/files/BBMap_39.01.tar.gz\ \ && \\\ntar xzf BBMap_39.01.tar.gz && \\\ncp -r bbmap/* /usr/local/bin\n" - type: "docker" run: - "bbsplit.sh --version 2>&1 | awk '/BBMap version/{print \"BBMAP:\", $NF}' >\ \ /var/software_versions.txt\n" entrypoint: [] cmd: null - type: "native" id: "native" build_info: config: "src/bbmap/bbmap_bbsplit/config.vsh.yaml" runner: "executable" engine: "docker|native" output: "target/executable/bbmap/bbmap_bbsplit" executable: "target/executable/bbmap/bbmap_bbsplit/bbmap_bbsplit" viash_version: "0.9.4" git_commit: "b0db228825f3441b4651527e8775e8fc87d06e60" git_remote: "https://github.com/viash-hub/biobox" git_tag: "v0.2.0-35-gb0db228" package_config: name: "biobox" version: "main" summary: "A curated collection of high-quality, standalone bioinformatics components\ \ built with [Viash](https://viash.io).\n" description: "`biobox` offers a suite of reliable bioinformatics components, similar\ \ to [nf-core/modules](https://github.com/nf-core/modules) and [snakemake-wrappers/bio](https://github.com/snakemake/snakemake-wrappers/tree/master/bio),\ \ but built using the [Viash](https://viash.io) framework.\n\nThis approach emphasizes\ \ **reusability**, **reproducibility**, and adherence to **best practices**. Key\ \ features of `biobox` components include:\n\n* **Standalone & Nextflow Ready:**\ \ Run components directly via the command line or seamlessly integrate them into\ \ Nextflow workflows.\n* **High Quality Standards:**\n * Comprehensive documentation\ \ for components and parameters.\n * Full exposure of underlying tool arguments.\n\ \ * Containerized (Docker) for dependency management and reproducibility.\n\ \ * Unit tested for verified functionality.\n" info: null viash_version: "0.9.4" source: "src" target: "target" config_mods: - ".requirements.commands := ['ps']\n" - ".engines += { type: \"native\" }" - ".engines[.type == 'docker'].target_registry := 'images.viash-hub.com'" - ".engines[.type == 'docker'].target_tag := 'main'" keywords: - "bioinformatics" - "modules" - "sequencing" license: "MIT" organization: "vsh" links: repository: "https://github.com/viash-hub/biobox" issue_tracker: "https://github.com/viash-hub/biobox/issues"