Build pipeline: viash-hub.biobox.main-zp6tq
Source commit: 7f8bcc2b3e
Source message: BD rhapsody sequence analysis (#96)
* wip
* fix test
* add help
* update 2.2 args
* fix bug
* extend test data
* output separate files
* analyse missing args
* tweaks to test
* fix script
* fix test
* fix test
* move small reference
* wip generate wta test data
* don't forget about umi in r1
* remove unneeded pkg
* load reference in memory just once
* fix random choices
* extend test
* add abc immunediscoverypanel
* wip abc testing code
* fix abc test; need unique instrument, run and flowcell ids for each sample
* add smk data
* add entry to changelog
* remove old test file
* adapt test for missing read
* update description
* add comment
* ensure cwl files are absolute
* Apply suggestions from code review
Co-authored-by: Dries Schaumont <5946712+DriesSchaumont@users.noreply.github.com>
* fix suggestion
* newer pipelines have docker requirements as a hint instead of a strict requirement
* rename str to content
* remove deleted resources
* fix containers
* fix script
* fix suggestion
* fix suggestion...
* fix test
* fix component name
* fix test
* apply suggestions
* fix test
* added note
* fix changelog
* fix changelog again
* splitting hairs here
---------
Co-authored-by: Dries Schaumont <5946712+DriesSchaumont@users.noreply.github.com>
365 lines
11 KiB
YAML
365 lines
11 KiB
YAML
name: "bedtools_genomecov"
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namespace: "bedtools"
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version: "main"
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authors:
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- name: "Theodoro Gasperin Terra Camargo"
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roles:
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- "author"
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- "maintainer"
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info:
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links:
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email: "theodorogtc@gmail.com"
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github: "tgaspe"
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linkedin: "theodoro-gasperin-terra-camargo"
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organizations:
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- name: "Data Intuitive"
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href: "https://www.data-intuitive.com"
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role: "Bioinformatician"
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argument_groups:
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- name: "Inputs"
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arguments:
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- type: "file"
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name: "--input"
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alternatives:
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- "-i"
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description: "The input file (BED/GFF/VCF) to be used.\n"
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info: null
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example:
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- "input.bed"
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must_exist: true
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create_parent: true
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required: false
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direction: "input"
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multiple: false
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multiple_sep: ";"
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- type: "file"
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name: "--input_bam"
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alternatives:
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- "-ibam"
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description: "The input file is in BAM format.\nNote: BAM _must_ be sorted by\
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\ positions.\n'--genome' option is ignored if you use '--input_bam' option!\n"
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info: null
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must_exist: true
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create_parent: true
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required: false
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direction: "input"
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multiple: false
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multiple_sep: ";"
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- type: "file"
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name: "--genome"
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alternatives:
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- "-g"
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description: "The genome file to be used.\n"
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info: null
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example:
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- "genome.txt"
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must_exist: true
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create_parent: true
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required: false
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direction: "input"
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multiple: false
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multiple_sep: ";"
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- name: "Outputs"
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arguments:
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- type: "file"
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name: "--output"
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description: "The output BED file. \n"
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info: null
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example:
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- "output.bed"
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must_exist: true
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create_parent: true
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required: true
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direction: "output"
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multiple: false
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multiple_sep: ";"
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- name: "Options"
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arguments:
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- type: "boolean_true"
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name: "--depth"
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alternatives:
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- "-d"
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description: "Report the depth at each genome position (with one-based coordinates).\n\
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Default behavior is to report a histogram.\n"
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info: null
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direction: "input"
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- type: "boolean_true"
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name: "--depth_zero"
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alternatives:
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- "-dz"
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description: "Report the depth at each genome position (with zero-based coordinates).\n\
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Reports only non-zero positions.\nDefault behavior is to report a histogram.\n"
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info: null
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direction: "input"
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- type: "boolean_true"
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name: "--bed_graph"
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alternatives:
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- "-bg"
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description: "Report depth in BedGraph format. For details, see:\ngenome.ucsc.edu/goldenPath/help/bedgraph.html\n"
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info: null
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direction: "input"
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- type: "boolean_true"
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name: "--bed_graph_zero_coverage"
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alternatives:
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- "-bga"
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description: "Report depth in BedGraph format, as above (-bg).\nHowever with this\
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\ option, regions with zero \ncoverage are also reported. This allows one to\n\
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quickly extract all regions of a genome with 0 \ncoverage by applying: \"grep\
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\ -w 0$\" to the output.\n"
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info: null
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direction: "input"
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- type: "boolean_true"
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name: "--split"
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description: "Treat \"split\" BAM or BED12 entries as distinct BED intervals.\n\
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when computing coverage.\nFor BAM files, this uses the CIGAR \"N\" and \"D\"\
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\ operations \nto infer the blocks for computing coverage.\nFor BED12 files,\
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\ this uses the BlockCount, BlockStarts, and BlockEnds\nfields (i.e., columns\
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\ 10,11,12).\n"
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info: null
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direction: "input"
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- type: "boolean_true"
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name: "--ignore_deletion"
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alternatives:
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- "-ignoreD"
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description: "Ignore local deletions (CIGAR \"D\" operations) in BAM entries\n\
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when computing coverage.\n"
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info: null
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direction: "input"
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- type: "string"
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name: "--strand"
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description: "Calculate coverage of intervals from a specific strand.\nWith BED\
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\ files, requires at least 6 columns (strand is column 6). \n"
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info: null
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required: false
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choices:
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- "+"
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- "-"
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direction: "input"
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multiple: false
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multiple_sep: ";"
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- type: "boolean_true"
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name: "--pair_end_coverage"
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alternatives:
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- "-pc"
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description: "Calculate coverage of pair-end fragments.\nWorks for BAM files only\n"
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info: null
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direction: "input"
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- type: "boolean_true"
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name: "--fragment_size"
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alternatives:
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- "-fs"
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description: "Force to use provided fragment size instead of read length\nWorks\
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\ for BAM files only\n"
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info: null
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direction: "input"
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- type: "boolean_true"
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name: "--du"
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description: "Change strand af the mate read (so both reads from the same strand)\
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\ useful for strand specific\nWorks for BAM files only\n"
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info: null
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direction: "input"
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- type: "boolean_true"
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name: "--five_prime"
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alternatives:
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- "-5"
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description: "Calculate coverage of 5\" positions (instead of entire interval).\n"
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info: null
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direction: "input"
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- type: "boolean_true"
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name: "--three_prime"
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alternatives:
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- "-3"
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description: "Calculate coverage of 3\" positions (instead of entire interval).\n"
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info: null
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direction: "input"
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- type: "integer"
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name: "--max"
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description: "Combine all positions with a depth >= max into\na single bin in\
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\ the histogram. Irrelevant\nfor -d and -bedGraph\n- (INTEGER)\n"
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info: null
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required: false
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min: 0
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direction: "input"
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multiple: false
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multiple_sep: ";"
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- type: "double"
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name: "--scale"
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description: "Scale the coverage by a constant factor.\nEach coverage value is\
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\ multiplied by this factor before being reported.\nUseful for normalizing coverage\
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\ by, e.g., reads per million (RPM).\n- Default is 1.0; i.e., unscaled.\n- (FLOAT)\n"
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info: null
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required: false
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min: 0.0
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direction: "input"
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multiple: false
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multiple_sep: ";"
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- type: "boolean_true"
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name: "--trackline"
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description: "Adds a UCSC/Genome-Browser track line definition in the first line\
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\ of the output.\n- See here for more details about track line definition:\n\
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\ http://genome.ucsc.edu/goldenPath/help/bedgraph.html\n- NOTE: When adding\
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\ a trackline definition, the output BedGraph can be easily\n uploaded\
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\ to the Genome Browser as a custom track,\n BUT CAN NOT be converted into\
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\ a BigWig file (w/o removing the first line).\n"
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info: null
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direction: "input"
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- type: "string"
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name: "--trackopts"
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description: "Writes additional track line definition parameters in the first\
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\ line.\n- Example:\n -trackopts 'name=\"My Track\" visibility=2 color=255,30,30'\n\
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\ Note the use of single-quotes if you have spaces in your parameters.\n- (TEXT)\n"
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info: null
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required: false
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direction: "input"
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multiple: true
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multiple_sep: ";"
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resources:
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- type: "bash_script"
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path: "script.sh"
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is_executable: true
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description: "Compute the coverage of a feature file among a genome.\n"
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test_resources:
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- type: "bash_script"
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path: "test.sh"
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is_executable: true
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- type: "file"
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path: "test_data"
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info: null
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status: "enabled"
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requirements:
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commands:
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- "ps"
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keywords:
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- "genome coverage"
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- "BED"
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- "GFF"
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- "VCF"
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- "BAM"
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license: "MIT"
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references:
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doi:
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- "10.1093/bioinformatics/btq033"
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links:
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repository: "https://github.com/arq5x/bedtools2"
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homepage: "https://bedtools.readthedocs.io/en/latest/#"
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documentation: "https://bedtools.readthedocs.io/en/latest/content/tools/genomecov.html"
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issue_tracker: "https://github.com/arq5x/bedtools2/issues"
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runners:
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- type: "executable"
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id: "executable"
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docker_setup_strategy: "ifneedbepullelsecachedbuild"
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- type: "nextflow"
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id: "nextflow"
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directives:
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tag: "$id"
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auto:
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simplifyInput: true
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simplifyOutput: false
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transcript: false
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publish: false
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config:
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labels:
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mem1gb: "memory = 1000000000.B"
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mem2gb: "memory = 2000000000.B"
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mem5gb: "memory = 5000000000.B"
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mem10gb: "memory = 10000000000.B"
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mem20gb: "memory = 20000000000.B"
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mem50gb: "memory = 50000000000.B"
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mem100gb: "memory = 100000000000.B"
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mem200gb: "memory = 200000000000.B"
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mem500gb: "memory = 500000000000.B"
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mem1tb: "memory = 1000000000000.B"
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mem2tb: "memory = 2000000000000.B"
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mem5tb: "memory = 5000000000000.B"
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mem10tb: "memory = 10000000000000.B"
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mem20tb: "memory = 20000000000000.B"
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mem50tb: "memory = 50000000000000.B"
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mem100tb: "memory = 100000000000000.B"
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mem200tb: "memory = 200000000000000.B"
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mem500tb: "memory = 500000000000000.B"
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mem1gib: "memory = 1073741824.B"
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mem2gib: "memory = 2147483648.B"
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mem4gib: "memory = 4294967296.B"
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mem8gib: "memory = 8589934592.B"
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mem16gib: "memory = 17179869184.B"
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mem32gib: "memory = 34359738368.B"
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mem64gib: "memory = 68719476736.B"
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mem128gib: "memory = 137438953472.B"
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mem256gib: "memory = 274877906944.B"
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mem512gib: "memory = 549755813888.B"
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mem1tib: "memory = 1099511627776.B"
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mem2tib: "memory = 2199023255552.B"
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mem4tib: "memory = 4398046511104.B"
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mem8tib: "memory = 8796093022208.B"
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mem16tib: "memory = 17592186044416.B"
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mem32tib: "memory = 35184372088832.B"
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mem64tib: "memory = 70368744177664.B"
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mem128tib: "memory = 140737488355328.B"
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mem256tib: "memory = 281474976710656.B"
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mem512tib: "memory = 562949953421312.B"
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cpu1: "cpus = 1"
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cpu2: "cpus = 2"
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cpu5: "cpus = 5"
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cpu10: "cpus = 10"
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cpu20: "cpus = 20"
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cpu50: "cpus = 50"
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cpu100: "cpus = 100"
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cpu200: "cpus = 200"
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cpu500: "cpus = 500"
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cpu1000: "cpus = 1000"
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debug: false
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container: "docker"
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engines:
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- type: "docker"
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id: "docker"
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image: "debian:stable-slim"
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target_registry: "images.viash-hub.com"
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target_tag: "main"
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namespace_separator: "/"
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setup:
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- type: "apt"
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packages:
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- "bedtools"
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- "procps"
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interactive: false
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- type: "docker"
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run:
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- "echo \"bedtools: \\\"$(bedtools --version | sed -n 's/^bedtools //p')\\\"\"\
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\ > /var/software_versions.txt\n"
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entrypoint: []
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cmd: null
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- type: "native"
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id: "native"
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build_info:
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config: "src/bedtools/bedtools_genomecov/config.vsh.yaml"
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runner: "executable"
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engine: "docker|native"
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output: "target/executable/bedtools/bedtools_genomecov"
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executable: "target/executable/bedtools/bedtools_genomecov/bedtools_genomecov"
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viash_version: "0.9.0"
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git_commit: "7f8bcc2b3e1ffaac9778b6acb42420b19660d1a1"
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git_remote: "https://x-access-token:ghs_aSDBedV4vU66pddFDN6d8UEy0ZQApn08RAsh@github.com/viash-hub/biobox"
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git_tag: "v0.2.0-3-g7f8bcc2"
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package_config:
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name: "biobox"
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version: "main"
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description: "A collection of bioinformatics tools for working with sequence data.\n"
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info: null
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viash_version: "0.9.0"
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source: "src"
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target: "target"
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config_mods:
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- ".requirements.commands := ['ps']\n"
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- ".engines += { type: \"native\" }"
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- ".engines[.type == 'docker'].target_registry := 'images.viash-hub.com'"
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- ".engines[.type == 'docker'].target_tag := 'main'"
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keywords:
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- "bioinformatics"
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- "modules"
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- "sequencing"
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license: "MIT"
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organization: "vsh"
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links:
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repository: "https://github.com/viash-hub/biobox"
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issue_tracker: "https://github.com/viash-hub/biobox/issues"
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