Files
biobox/target/executable/bedtools/bedtools_genomecov/.config.vsh.yaml
CI 6f2f840fd9 Build branch main with version main (7f8bcc2)
Build pipeline: viash-hub.biobox.main-zp6tq

Source commit: 7f8bcc2b3e

Source message: BD rhapsody sequence analysis (#96)

* wip

* fix test

* add help

* update 2.2 args

* fix bug

* extend test data

* output separate files

* analyse missing args

* tweaks to test

* fix script

* fix test

* fix test

* move small reference

* wip generate wta test data

* don't forget about umi in r1

* remove unneeded pkg

* load reference in memory just once

* fix random choices

* extend test

* add abc immunediscoverypanel

* wip abc testing code

* fix abc test; need unique instrument, run and flowcell ids for each sample

* add smk data

* add entry to changelog

* remove old test file

* adapt test for missing read

* update description

* add comment

* ensure cwl files are absolute

* Apply suggestions from code review

Co-authored-by: Dries Schaumont <5946712+DriesSchaumont@users.noreply.github.com>

* fix suggestion

* newer pipelines have docker requirements as a hint instead of a strict requirement

* rename str to content

* remove deleted resources

* fix containers

* fix script

* fix suggestion

* fix suggestion...

* fix test

* fix component name

* fix test

* apply suggestions

* fix test

* added note

* fix changelog

* fix changelog again

* splitting hairs here

---------

Co-authored-by: Dries Schaumont <5946712+DriesSchaumont@users.noreply.github.com>
2024-09-17 10:13:18 +00:00

365 lines
11 KiB
YAML

name: "bedtools_genomecov"
namespace: "bedtools"
version: "main"
authors:
- name: "Theodoro Gasperin Terra Camargo"
roles:
- "author"
- "maintainer"
info:
links:
email: "theodorogtc@gmail.com"
github: "tgaspe"
linkedin: "theodoro-gasperin-terra-camargo"
organizations:
- name: "Data Intuitive"
href: "https://www.data-intuitive.com"
role: "Bioinformatician"
argument_groups:
- name: "Inputs"
arguments:
- type: "file"
name: "--input"
alternatives:
- "-i"
description: "The input file (BED/GFF/VCF) to be used.\n"
info: null
example:
- "input.bed"
must_exist: true
create_parent: true
required: false
direction: "input"
multiple: false
multiple_sep: ";"
- type: "file"
name: "--input_bam"
alternatives:
- "-ibam"
description: "The input file is in BAM format.\nNote: BAM _must_ be sorted by\
\ positions.\n'--genome' option is ignored if you use '--input_bam' option!\n"
info: null
must_exist: true
create_parent: true
required: false
direction: "input"
multiple: false
multiple_sep: ";"
- type: "file"
name: "--genome"
alternatives:
- "-g"
description: "The genome file to be used.\n"
info: null
example:
- "genome.txt"
must_exist: true
create_parent: true
required: false
direction: "input"
multiple: false
multiple_sep: ";"
- name: "Outputs"
arguments:
- type: "file"
name: "--output"
description: "The output BED file. \n"
info: null
example:
- "output.bed"
must_exist: true
create_parent: true
required: true
direction: "output"
multiple: false
multiple_sep: ";"
- name: "Options"
arguments:
- type: "boolean_true"
name: "--depth"
alternatives:
- "-d"
description: "Report the depth at each genome position (with one-based coordinates).\n\
Default behavior is to report a histogram.\n"
info: null
direction: "input"
- type: "boolean_true"
name: "--depth_zero"
alternatives:
- "-dz"
description: "Report the depth at each genome position (with zero-based coordinates).\n\
Reports only non-zero positions.\nDefault behavior is to report a histogram.\n"
info: null
direction: "input"
- type: "boolean_true"
name: "--bed_graph"
alternatives:
- "-bg"
description: "Report depth in BedGraph format. For details, see:\ngenome.ucsc.edu/goldenPath/help/bedgraph.html\n"
info: null
direction: "input"
- type: "boolean_true"
name: "--bed_graph_zero_coverage"
alternatives:
- "-bga"
description: "Report depth in BedGraph format, as above (-bg).\nHowever with this\
\ option, regions with zero \ncoverage are also reported. This allows one to\n\
quickly extract all regions of a genome with 0 \ncoverage by applying: \"grep\
\ -w 0$\" to the output.\n"
info: null
direction: "input"
- type: "boolean_true"
name: "--split"
description: "Treat \"split\" BAM or BED12 entries as distinct BED intervals.\n\
when computing coverage.\nFor BAM files, this uses the CIGAR \"N\" and \"D\"\
\ operations \nto infer the blocks for computing coverage.\nFor BED12 files,\
\ this uses the BlockCount, BlockStarts, and BlockEnds\nfields (i.e., columns\
\ 10,11,12).\n"
info: null
direction: "input"
- type: "boolean_true"
name: "--ignore_deletion"
alternatives:
- "-ignoreD"
description: "Ignore local deletions (CIGAR \"D\" operations) in BAM entries\n\
when computing coverage.\n"
info: null
direction: "input"
- type: "string"
name: "--strand"
description: "Calculate coverage of intervals from a specific strand.\nWith BED\
\ files, requires at least 6 columns (strand is column 6). \n"
info: null
required: false
choices:
- "+"
- "-"
direction: "input"
multiple: false
multiple_sep: ";"
- type: "boolean_true"
name: "--pair_end_coverage"
alternatives:
- "-pc"
description: "Calculate coverage of pair-end fragments.\nWorks for BAM files only\n"
info: null
direction: "input"
- type: "boolean_true"
name: "--fragment_size"
alternatives:
- "-fs"
description: "Force to use provided fragment size instead of read length\nWorks\
\ for BAM files only\n"
info: null
direction: "input"
- type: "boolean_true"
name: "--du"
description: "Change strand af the mate read (so both reads from the same strand)\
\ useful for strand specific\nWorks for BAM files only\n"
info: null
direction: "input"
- type: "boolean_true"
name: "--five_prime"
alternatives:
- "-5"
description: "Calculate coverage of 5\" positions (instead of entire interval).\n"
info: null
direction: "input"
- type: "boolean_true"
name: "--three_prime"
alternatives:
- "-3"
description: "Calculate coverage of 3\" positions (instead of entire interval).\n"
info: null
direction: "input"
- type: "integer"
name: "--max"
description: "Combine all positions with a depth >= max into\na single bin in\
\ the histogram. Irrelevant\nfor -d and -bedGraph\n- (INTEGER)\n"
info: null
required: false
min: 0
direction: "input"
multiple: false
multiple_sep: ";"
- type: "double"
name: "--scale"
description: "Scale the coverage by a constant factor.\nEach coverage value is\
\ multiplied by this factor before being reported.\nUseful for normalizing coverage\
\ by, e.g., reads per million (RPM).\n- Default is 1.0; i.e., unscaled.\n- (FLOAT)\n"
info: null
required: false
min: 0.0
direction: "input"
multiple: false
multiple_sep: ";"
- type: "boolean_true"
name: "--trackline"
description: "Adds a UCSC/Genome-Browser track line definition in the first line\
\ of the output.\n- See here for more details about track line definition:\n\
\ http://genome.ucsc.edu/goldenPath/help/bedgraph.html\n- NOTE: When adding\
\ a trackline definition, the output BedGraph can be easily\n uploaded\
\ to the Genome Browser as a custom track,\n BUT CAN NOT be converted into\
\ a BigWig file (w/o removing the first line).\n"
info: null
direction: "input"
- type: "string"
name: "--trackopts"
description: "Writes additional track line definition parameters in the first\
\ line.\n- Example:\n -trackopts 'name=\"My Track\" visibility=2 color=255,30,30'\n\
\ Note the use of single-quotes if you have spaces in your parameters.\n- (TEXT)\n"
info: null
required: false
direction: "input"
multiple: true
multiple_sep: ";"
resources:
- type: "bash_script"
path: "script.sh"
is_executable: true
description: "Compute the coverage of a feature file among a genome.\n"
test_resources:
- type: "bash_script"
path: "test.sh"
is_executable: true
- type: "file"
path: "test_data"
info: null
status: "enabled"
requirements:
commands:
- "ps"
keywords:
- "genome coverage"
- "BED"
- "GFF"
- "VCF"
- "BAM"
license: "MIT"
references:
doi:
- "10.1093/bioinformatics/btq033"
links:
repository: "https://github.com/arq5x/bedtools2"
homepage: "https://bedtools.readthedocs.io/en/latest/#"
documentation: "https://bedtools.readthedocs.io/en/latest/content/tools/genomecov.html"
issue_tracker: "https://github.com/arq5x/bedtools2/issues"
runners:
- type: "executable"
id: "executable"
docker_setup_strategy: "ifneedbepullelsecachedbuild"
- type: "nextflow"
id: "nextflow"
directives:
tag: "$id"
auto:
simplifyInput: true
simplifyOutput: false
transcript: false
publish: false
config:
labels:
mem1gb: "memory = 1000000000.B"
mem2gb: "memory = 2000000000.B"
mem5gb: "memory = 5000000000.B"
mem10gb: "memory = 10000000000.B"
mem20gb: "memory = 20000000000.B"
mem50gb: "memory = 50000000000.B"
mem100gb: "memory = 100000000000.B"
mem200gb: "memory = 200000000000.B"
mem500gb: "memory = 500000000000.B"
mem1tb: "memory = 1000000000000.B"
mem2tb: "memory = 2000000000000.B"
mem5tb: "memory = 5000000000000.B"
mem10tb: "memory = 10000000000000.B"
mem20tb: "memory = 20000000000000.B"
mem50tb: "memory = 50000000000000.B"
mem100tb: "memory = 100000000000000.B"
mem200tb: "memory = 200000000000000.B"
mem500tb: "memory = 500000000000000.B"
mem1gib: "memory = 1073741824.B"
mem2gib: "memory = 2147483648.B"
mem4gib: "memory = 4294967296.B"
mem8gib: "memory = 8589934592.B"
mem16gib: "memory = 17179869184.B"
mem32gib: "memory = 34359738368.B"
mem64gib: "memory = 68719476736.B"
mem128gib: "memory = 137438953472.B"
mem256gib: "memory = 274877906944.B"
mem512gib: "memory = 549755813888.B"
mem1tib: "memory = 1099511627776.B"
mem2tib: "memory = 2199023255552.B"
mem4tib: "memory = 4398046511104.B"
mem8tib: "memory = 8796093022208.B"
mem16tib: "memory = 17592186044416.B"
mem32tib: "memory = 35184372088832.B"
mem64tib: "memory = 70368744177664.B"
mem128tib: "memory = 140737488355328.B"
mem256tib: "memory = 281474976710656.B"
mem512tib: "memory = 562949953421312.B"
cpu1: "cpus = 1"
cpu2: "cpus = 2"
cpu5: "cpus = 5"
cpu10: "cpus = 10"
cpu20: "cpus = 20"
cpu50: "cpus = 50"
cpu100: "cpus = 100"
cpu200: "cpus = 200"
cpu500: "cpus = 500"
cpu1000: "cpus = 1000"
debug: false
container: "docker"
engines:
- type: "docker"
id: "docker"
image: "debian:stable-slim"
target_registry: "images.viash-hub.com"
target_tag: "main"
namespace_separator: "/"
setup:
- type: "apt"
packages:
- "bedtools"
- "procps"
interactive: false
- type: "docker"
run:
- "echo \"bedtools: \\\"$(bedtools --version | sed -n 's/^bedtools //p')\\\"\"\
\ > /var/software_versions.txt\n"
entrypoint: []
cmd: null
- type: "native"
id: "native"
build_info:
config: "src/bedtools/bedtools_genomecov/config.vsh.yaml"
runner: "executable"
engine: "docker|native"
output: "target/executable/bedtools/bedtools_genomecov"
executable: "target/executable/bedtools/bedtools_genomecov/bedtools_genomecov"
viash_version: "0.9.0"
git_commit: "7f8bcc2b3e1ffaac9778b6acb42420b19660d1a1"
git_remote: "https://x-access-token:ghs_aSDBedV4vU66pddFDN6d8UEy0ZQApn08RAsh@github.com/viash-hub/biobox"
git_tag: "v0.2.0-3-g7f8bcc2"
package_config:
name: "biobox"
version: "main"
description: "A collection of bioinformatics tools for working with sequence data.\n"
info: null
viash_version: "0.9.0"
source: "src"
target: "target"
config_mods:
- ".requirements.commands := ['ps']\n"
- ".engines += { type: \"native\" }"
- ".engines[.type == 'docker'].target_registry := 'images.viash-hub.com'"
- ".engines[.type == 'docker'].target_tag := 'main'"
keywords:
- "bioinformatics"
- "modules"
- "sequencing"
license: "MIT"
organization: "vsh"
links:
repository: "https://github.com/viash-hub/biobox"
issue_tracker: "https://github.com/viash-hub/biobox/issues"