Build pipeline: viash-hub.biobox.main-zp6tq
Source commit: 7f8bcc2b3e
Source message: BD rhapsody sequence analysis (#96)
* wip
* fix test
* add help
* update 2.2 args
* fix bug
* extend test data
* output separate files
* analyse missing args
* tweaks to test
* fix script
* fix test
* fix test
* move small reference
* wip generate wta test data
* don't forget about umi in r1
* remove unneeded pkg
* load reference in memory just once
* fix random choices
* extend test
* add abc immunediscoverypanel
* wip abc testing code
* fix abc test; need unique instrument, run and flowcell ids for each sample
* add smk data
* add entry to changelog
* remove old test file
* adapt test for missing read
* update description
* add comment
* ensure cwl files are absolute
* Apply suggestions from code review
Co-authored-by: Dries Schaumont <5946712+DriesSchaumont@users.noreply.github.com>
* fix suggestion
* newer pipelines have docker requirements as a hint instead of a strict requirement
* rename str to content
* remove deleted resources
* fix containers
* fix script
* fix suggestion
* fix suggestion...
* fix test
* fix component name
* fix test
* apply suggestions
* fix test
* added note
* fix changelog
* fix changelog again
* splitting hairs here
---------
Co-authored-by: Dries Schaumont <5946712+DriesSchaumont@users.noreply.github.com>
345 lines
11 KiB
YAML
345 lines
11 KiB
YAML
name: "falco"
|
|
version: "main"
|
|
authors:
|
|
- name: "Toni Verbeiren"
|
|
roles:
|
|
- "author"
|
|
- "maintainer"
|
|
info:
|
|
links:
|
|
github: "tverbeiren"
|
|
linkedin: "verbeiren"
|
|
organizations:
|
|
- name: "Data Intuitive"
|
|
href: "https://www.data-intuitive.com"
|
|
role: "Data Scientist and CEO"
|
|
argument_groups:
|
|
- name: "Input arguments"
|
|
arguments:
|
|
- type: "file"
|
|
name: "--input"
|
|
description: "input fastq files"
|
|
info: null
|
|
example:
|
|
- "input1.fastq;input2.fastq"
|
|
must_exist: true
|
|
create_parent: true
|
|
required: true
|
|
direction: "input"
|
|
multiple: true
|
|
multiple_sep: ";"
|
|
- name: "Run arguments"
|
|
arguments:
|
|
- type: "boolean_true"
|
|
name: "--nogroup"
|
|
description: "Disable grouping of bases for reads >50bp. \nAll reports will show\
|
|
\ data for every base in \nthe read. WARNING: When using this option, \nyour\
|
|
\ plots may end up a ridiculous size. You \nhave been warned!\n"
|
|
info: null
|
|
direction: "input"
|
|
- type: "file"
|
|
name: "--contaminents"
|
|
description: "Specifies a non-default file which contains \nthe list of contaminants\
|
|
\ to screen \noverrepresented sequences against. The file \nmust contain sets\
|
|
\ of named contaminants in \nthe form name[tab]sequence. Lines prefixed \nwith\
|
|
\ a hash will be ignored. Default: \nhttps://github.com/smithlabcode/falco/blob/v1.2.2/Configuration/contaminant_list.txt\n"
|
|
info: null
|
|
must_exist: true
|
|
create_parent: true
|
|
required: false
|
|
direction: "input"
|
|
multiple: false
|
|
multiple_sep: ";"
|
|
- type: "file"
|
|
name: "--adapters"
|
|
description: "Specifies a non-default file which contains \nthe list of adapter\
|
|
\ sequences which will be \nexplicity searched against the library. The \nfile\
|
|
\ must contain sets of named adapters in \nthe form name[tab]sequence. Lines\
|
|
\ prefixed \nwith a hash will be ignored. Default:\nhttps://github.com/smithlabcode/falco/blob/v1.2.2/Configuration/adapter_list.txt\n"
|
|
info: null
|
|
must_exist: true
|
|
create_parent: true
|
|
required: false
|
|
direction: "input"
|
|
multiple: false
|
|
multiple_sep: ";"
|
|
- type: "file"
|
|
name: "--limits"
|
|
description: "Specifies a non-default file which contains \na set of criteria\
|
|
\ which will be used to \ndetermine the warn/error limits for the \nvarious\
|
|
\ modules. This file can also be used \nto selectively remove some modules from\
|
|
\ the \noutput all together. The format needs to \nmirror the default limits.txt\
|
|
\ file found in \nthe Configuration folder. Default: \nhttps://github.com/smithlabcode/falco/blob/v1.2.2/Configuration/limits.txt\n"
|
|
info: null
|
|
must_exist: true
|
|
create_parent: true
|
|
required: false
|
|
direction: "input"
|
|
multiple: false
|
|
multiple_sep: ";"
|
|
- type: "integer"
|
|
name: "--subsample"
|
|
alternatives:
|
|
- "-s"
|
|
description: "[Falco only] makes falco faster (but \npossibly less accurate) by\
|
|
\ only processing \nreads that are a multiple of this value (using \n0-based\
|
|
\ indexing to number reads).\n"
|
|
info: null
|
|
example:
|
|
- 10
|
|
required: false
|
|
direction: "input"
|
|
multiple: false
|
|
multiple_sep: ";"
|
|
- type: "boolean_true"
|
|
name: "--bisulfite"
|
|
alternatives:
|
|
- "-b"
|
|
description: "[Falco only] reads are whole genome \nbisulfite sequencing, and\
|
|
\ more Ts and fewer \nCs are therefore expected and will be \naccounted for\
|
|
\ in base content.\n"
|
|
info: null
|
|
direction: "input"
|
|
- type: "boolean_true"
|
|
name: "--reverse_complliment"
|
|
alternatives:
|
|
- "-r"
|
|
description: "[Falco only] The input is a \nreverse-complement. All modules will\
|
|
\ be \ntested by swapping A/T and C/G\n"
|
|
info: null
|
|
direction: "input"
|
|
- name: "Output arguments"
|
|
arguments:
|
|
- type: "file"
|
|
name: "--outdir"
|
|
alternatives:
|
|
- "-o"
|
|
description: "Create all output files in the specified \noutput directory. FALCO-SPECIFIC:\
|
|
\ If the \ndirectory does not exists, the program will \ncreate it.\n"
|
|
info: null
|
|
example:
|
|
- "output"
|
|
must_exist: true
|
|
create_parent: true
|
|
required: true
|
|
direction: "output"
|
|
multiple: false
|
|
multiple_sep: ";"
|
|
- type: "string"
|
|
name: "--format"
|
|
alternatives:
|
|
- "-f"
|
|
description: "Bypasses the normal sequence file format \ndetection and forces\
|
|
\ the program to use the \nspecified format. Validformats are bam, sam, \nbam_mapped,\
|
|
\ sam_mapped, fastq, fq, fastq.gz \nor fq.gz.\n"
|
|
info: null
|
|
required: false
|
|
choices:
|
|
- "bam"
|
|
- "sam"
|
|
- "bam_mapped"
|
|
- "sam_mapped"
|
|
- "fastq"
|
|
- "fq"
|
|
- "fastq.gz"
|
|
- "fq.gz"
|
|
direction: "input"
|
|
multiple: false
|
|
multiple_sep: ";"
|
|
- type: "file"
|
|
name: "--data_filename"
|
|
alternatives:
|
|
- "-D"
|
|
description: "[Falco only] Specify filename for FastQC \ndata output (TXT). If\
|
|
\ not specified, it will \nbe called fastq_data.txt in either the input \nfile's\
|
|
\ directory or the one specified in the \n--output flag. Only available when\
|
|
\ running \nfalco with a single input.\n"
|
|
info: null
|
|
must_exist: true
|
|
create_parent: true
|
|
required: false
|
|
direction: "output"
|
|
multiple: false
|
|
multiple_sep: ";"
|
|
- type: "file"
|
|
name: "--report_filename"
|
|
alternatives:
|
|
- "-R"
|
|
description: "[Falco only] Specify filename for FastQC \nreport output (HTML).\
|
|
\ If not specified, it \nwill be called fastq_report.html in either \nthe input\
|
|
\ file's directory or the one \nspecified in the --output flag. Only \navailable\
|
|
\ when running falco with a single \ninput.\n"
|
|
info: null
|
|
must_exist: true
|
|
create_parent: true
|
|
required: false
|
|
direction: "output"
|
|
multiple: false
|
|
multiple_sep: ";"
|
|
- type: "file"
|
|
name: "--summary_filename"
|
|
alternatives:
|
|
- "-S"
|
|
description: "[Falco only] Specify filename for the short \nsummary output (TXT).\
|
|
\ If not specified, it \nwill be called fastq_report.html in either \nthe input\
|
|
\ file's directory or the one \nspecified in the --output flag. Only \navailable\
|
|
\ when running falco with a single \ninput.\n"
|
|
info: null
|
|
must_exist: true
|
|
create_parent: true
|
|
required: false
|
|
direction: "output"
|
|
multiple: false
|
|
multiple_sep: ";"
|
|
resources:
|
|
- type: "bash_script"
|
|
path: "script.sh"
|
|
is_executable: true
|
|
description: "A C++ drop-in replacement of FastQC to assess the quality of sequence\
|
|
\ read data"
|
|
test_resources:
|
|
- type: "bash_script"
|
|
path: "test.sh"
|
|
is_executable: true
|
|
info: null
|
|
status: "enabled"
|
|
requirements:
|
|
commands:
|
|
- "ps"
|
|
keywords:
|
|
- "qc"
|
|
- "fastqc"
|
|
- "sequencing"
|
|
license: "GPL-3.0"
|
|
references:
|
|
doi:
|
|
- "10.12688/f1000research.21142.2"
|
|
links:
|
|
repository: "https://github.com/smithlabcode/falco"
|
|
documentation: "https://falco.readthedocs.io/en/latest/"
|
|
runners:
|
|
- type: "executable"
|
|
id: "executable"
|
|
docker_setup_strategy: "ifneedbepullelsecachedbuild"
|
|
- type: "nextflow"
|
|
id: "nextflow"
|
|
directives:
|
|
tag: "$id"
|
|
auto:
|
|
simplifyInput: true
|
|
simplifyOutput: false
|
|
transcript: false
|
|
publish: false
|
|
config:
|
|
labels:
|
|
mem1gb: "memory = 1000000000.B"
|
|
mem2gb: "memory = 2000000000.B"
|
|
mem5gb: "memory = 5000000000.B"
|
|
mem10gb: "memory = 10000000000.B"
|
|
mem20gb: "memory = 20000000000.B"
|
|
mem50gb: "memory = 50000000000.B"
|
|
mem100gb: "memory = 100000000000.B"
|
|
mem200gb: "memory = 200000000000.B"
|
|
mem500gb: "memory = 500000000000.B"
|
|
mem1tb: "memory = 1000000000000.B"
|
|
mem2tb: "memory = 2000000000000.B"
|
|
mem5tb: "memory = 5000000000000.B"
|
|
mem10tb: "memory = 10000000000000.B"
|
|
mem20tb: "memory = 20000000000000.B"
|
|
mem50tb: "memory = 50000000000000.B"
|
|
mem100tb: "memory = 100000000000000.B"
|
|
mem200tb: "memory = 200000000000000.B"
|
|
mem500tb: "memory = 500000000000000.B"
|
|
mem1gib: "memory = 1073741824.B"
|
|
mem2gib: "memory = 2147483648.B"
|
|
mem4gib: "memory = 4294967296.B"
|
|
mem8gib: "memory = 8589934592.B"
|
|
mem16gib: "memory = 17179869184.B"
|
|
mem32gib: "memory = 34359738368.B"
|
|
mem64gib: "memory = 68719476736.B"
|
|
mem128gib: "memory = 137438953472.B"
|
|
mem256gib: "memory = 274877906944.B"
|
|
mem512gib: "memory = 549755813888.B"
|
|
mem1tib: "memory = 1099511627776.B"
|
|
mem2tib: "memory = 2199023255552.B"
|
|
mem4tib: "memory = 4398046511104.B"
|
|
mem8tib: "memory = 8796093022208.B"
|
|
mem16tib: "memory = 17592186044416.B"
|
|
mem32tib: "memory = 35184372088832.B"
|
|
mem64tib: "memory = 70368744177664.B"
|
|
mem128tib: "memory = 140737488355328.B"
|
|
mem256tib: "memory = 281474976710656.B"
|
|
mem512tib: "memory = 562949953421312.B"
|
|
cpu1: "cpus = 1"
|
|
cpu2: "cpus = 2"
|
|
cpu5: "cpus = 5"
|
|
cpu10: "cpus = 10"
|
|
cpu20: "cpus = 20"
|
|
cpu50: "cpus = 50"
|
|
cpu100: "cpus = 100"
|
|
cpu200: "cpus = 200"
|
|
cpu500: "cpus = 500"
|
|
cpu1000: "cpus = 1000"
|
|
debug: false
|
|
container: "docker"
|
|
engines:
|
|
- type: "docker"
|
|
id: "docker"
|
|
image: "debian:trixie-slim"
|
|
target_registry: "images.viash-hub.com"
|
|
target_tag: "main"
|
|
namespace_separator: "/"
|
|
setup:
|
|
- type: "apt"
|
|
packages:
|
|
- "wget"
|
|
- "build-essential"
|
|
- "g++"
|
|
- "zlib1g-dev"
|
|
- "procps"
|
|
interactive: false
|
|
- type: "docker"
|
|
run:
|
|
- "wget https://github.com/smithlabcode/falco/releases/download/v1.2.2/falco-1.2.2.tar.gz\
|
|
\ -O /tmp/falco.tar.gz && \\\ncd /tmp && \\\ntar xvf falco.tar.gz && \\\ncd\
|
|
\ falco-1.2.2 && \\\n./configure && \\\nmake all && \\\nmake install\n"
|
|
- type: "docker"
|
|
run:
|
|
- "echo \"falco: \\\"$(falco -v | sed -n 's/^falco //p')\\\"\" > /var/software_versions.txt\n"
|
|
entrypoint: []
|
|
cmd: null
|
|
- type: "native"
|
|
id: "native"
|
|
build_info:
|
|
config: "src/falco/config.vsh.yaml"
|
|
runner: "executable"
|
|
engine: "docker|native"
|
|
output: "target/executable/falco"
|
|
executable: "target/executable/falco/falco"
|
|
viash_version: "0.9.0"
|
|
git_commit: "7f8bcc2b3e1ffaac9778b6acb42420b19660d1a1"
|
|
git_remote: "https://x-access-token:ghs_aSDBedV4vU66pddFDN6d8UEy0ZQApn08RAsh@github.com/viash-hub/biobox"
|
|
git_tag: "v0.2.0-3-g7f8bcc2"
|
|
package_config:
|
|
name: "biobox"
|
|
version: "main"
|
|
description: "A collection of bioinformatics tools for working with sequence data.\n"
|
|
info: null
|
|
viash_version: "0.9.0"
|
|
source: "src"
|
|
target: "target"
|
|
config_mods:
|
|
- ".requirements.commands := ['ps']\n"
|
|
- ".engines += { type: \"native\" }"
|
|
- ".engines[.type == 'docker'].target_registry := 'images.viash-hub.com'"
|
|
- ".engines[.type == 'docker'].target_tag := 'main'"
|
|
keywords:
|
|
- "bioinformatics"
|
|
- "modules"
|
|
- "sequencing"
|
|
license: "MIT"
|
|
organization: "vsh"
|
|
links:
|
|
repository: "https://github.com/viash-hub/biobox"
|
|
issue_tracker: "https://github.com/viash-hub/biobox/issues"
|