Build pipeline: viash-hub.biobox.main-zp6tq
Source commit: 7f8bcc2b3e
Source message: BD rhapsody sequence analysis (#96)
* wip
* fix test
* add help
* update 2.2 args
* fix bug
* extend test data
* output separate files
* analyse missing args
* tweaks to test
* fix script
* fix test
* fix test
* move small reference
* wip generate wta test data
* don't forget about umi in r1
* remove unneeded pkg
* load reference in memory just once
* fix random choices
* extend test
* add abc immunediscoverypanel
* wip abc testing code
* fix abc test; need unique instrument, run and flowcell ids for each sample
* add smk data
* add entry to changelog
* remove old test file
* adapt test for missing read
* update description
* add comment
* ensure cwl files are absolute
* Apply suggestions from code review
Co-authored-by: Dries Schaumont <5946712+DriesSchaumont@users.noreply.github.com>
* fix suggestion
* newer pipelines have docker requirements as a hint instead of a strict requirement
* rename str to content
* remove deleted resources
* fix containers
* fix script
* fix suggestion
* fix suggestion...
* fix test
* fix component name
* fix test
* apply suggestions
* fix test
* added note
* fix changelog
* fix changelog again
* splitting hairs here
---------
Co-authored-by: Dries Schaumont <5946712+DriesSchaumont@users.noreply.github.com>
368 lines
12 KiB
YAML
368 lines
12 KiB
YAML
name: "fastqc"
|
|
version: "main"
|
|
authors:
|
|
- name: "Theodoro Gasperin Terra Camargo"
|
|
roles:
|
|
- "author"
|
|
- "maintainer"
|
|
info:
|
|
links:
|
|
email: "theodorogtc@gmail.com"
|
|
github: "tgaspe"
|
|
linkedin: "theodoro-gasperin-terra-camargo"
|
|
organizations:
|
|
- name: "Data Intuitive"
|
|
href: "https://www.data-intuitive.com"
|
|
role: "Bioinformatician"
|
|
argument_groups:
|
|
- name: "Inputs"
|
|
arguments:
|
|
- type: "file"
|
|
name: "--input"
|
|
description: "FASTQ file(s) to be analyzed.\n"
|
|
info: null
|
|
example:
|
|
- "input.fq"
|
|
must_exist: true
|
|
create_parent: true
|
|
required: true
|
|
direction: "input"
|
|
multiple: true
|
|
multiple_sep: ";"
|
|
- name: "Outputs"
|
|
description: "At least one of the output options (--html, --zip, --summary, --data)\
|
|
\ must be used.\n"
|
|
arguments:
|
|
- type: "file"
|
|
name: "--html"
|
|
description: "Create the HTML report of the results. \n'*' wild card must be provided\
|
|
\ in the output file name. \nWild card will be replaced by the input file basename.\n\
|
|
e.g. \n --input \"sample_1.fq\"\n --html \"*.html\"\n would create an output\
|
|
\ html file named sample_1.html\n"
|
|
info: null
|
|
example:
|
|
- "*.html"
|
|
must_exist: true
|
|
create_parent: true
|
|
required: false
|
|
direction: "output"
|
|
multiple: true
|
|
multiple_sep: ";"
|
|
- type: "file"
|
|
name: "--zip"
|
|
description: "Create the zip file(s) containing: html report, data, images, icons,\
|
|
\ summary, etc.\n'*' wild card must be provided in the output file name.\nWild\
|
|
\ card will be replaced by the input basename.\ne.g. \n --input \"sample_1.fq\"\
|
|
\n --html \"*.zip\"\n would create an output zip file named sample_1.zip\n"
|
|
info: null
|
|
example:
|
|
- "*.zip"
|
|
must_exist: true
|
|
create_parent: true
|
|
required: false
|
|
direction: "output"
|
|
multiple: true
|
|
multiple_sep: ";"
|
|
- type: "file"
|
|
name: "--summary"
|
|
description: "Create the summary file(s).\n'*' wild card must be provided in the\
|
|
\ output file name.\nWild card will be replaced by the input basename.\ne.g.\
|
|
\ \n --input \"sample_1.fq\"\n --summary \"*_summary.txt\"\n would create\
|
|
\ an output summary.txt file named sample_1_summary.txt\n"
|
|
info: null
|
|
example:
|
|
- "*_summary.txt"
|
|
must_exist: true
|
|
create_parent: true
|
|
required: false
|
|
direction: "output"
|
|
multiple: true
|
|
multiple_sep: ";"
|
|
- type: "file"
|
|
name: "--data"
|
|
description: "Create the data file(s).\n'*' wild card must be provided in the\
|
|
\ output file name.\nWild card will be replaced by the input basename.\ne.g.\
|
|
\ \n --input \"sample_1.fq\"\n --summary \"*_data.txt\"\n would create an\
|
|
\ output data.txt file named sample_1_data.txt\n"
|
|
info: null
|
|
example:
|
|
- "*_data.txt"
|
|
must_exist: true
|
|
create_parent: true
|
|
required: false
|
|
direction: "output"
|
|
multiple: true
|
|
multiple_sep: ";"
|
|
- name: "Options"
|
|
arguments:
|
|
- type: "boolean_true"
|
|
name: "--casava"
|
|
description: "Files come from raw casava output. Files in the same sample\ngroup\
|
|
\ (differing only by the group number) will be analysed\nas a set rather than\
|
|
\ individually. Sequences with the filter\nflag set in the header will be excluded\
|
|
\ from the analysis.\nFiles must have the same names given to them by casava\n\
|
|
(including being gzipped and ending with .gz) otherwise they\nwon't be grouped\
|
|
\ together correctly.\n"
|
|
info: null
|
|
direction: "input"
|
|
- type: "boolean_true"
|
|
name: "--nano"
|
|
description: "Files come from nanopore sequences and are in fast5 format. In\n\
|
|
this mode you can pass in directories to process and the program\nwill take\
|
|
\ in all fast5 files within those directories and produce\na single output file\
|
|
\ from the sequences found in all files.\n"
|
|
info: null
|
|
direction: "input"
|
|
- type: "boolean_true"
|
|
name: "--nofilter"
|
|
description: "If running with --casava then don't remove read flagged by\ncasava\
|
|
\ as poor quality when performing the QC analysis.\n"
|
|
info: null
|
|
direction: "input"
|
|
- type: "boolean_true"
|
|
name: "--nogroup"
|
|
description: "Disable grouping of bases for reads >50bp. \nAll reports will show\
|
|
\ data for every base in the read. \nWARNING: Using this option will cause fastqc\
|
|
\ to crash \nand burn if you use it on really long reads, and your \nplots may\
|
|
\ end up a ridiculous size. You have been warned!\n"
|
|
info: null
|
|
direction: "input"
|
|
- type: "integer"
|
|
name: "--min_length"
|
|
description: "Sets an artificial lower limit on the length of the \nsequence to\
|
|
\ be shown in the report. As long as you \nset this to a value greater or equal\
|
|
\ to your longest \nread length then this will be the sequence length used \n\
|
|
to create your read groups. This can be useful for making\ndirectly comparable\
|
|
\ statistics from datasets with somewhat \nvariable read lengths.\n"
|
|
info: null
|
|
example:
|
|
- 0
|
|
required: false
|
|
direction: "input"
|
|
multiple: false
|
|
multiple_sep: ";"
|
|
- type: "string"
|
|
name: "--format"
|
|
alternatives:
|
|
- "-f"
|
|
description: "Bypasses the normal sequence file format detection and \nforces\
|
|
\ the program to use the specified format. \nValid formats are bam, sam, bam_mapped,\
|
|
\ sam_mapped, and fastq.\n"
|
|
info: null
|
|
example:
|
|
- "bam"
|
|
required: false
|
|
direction: "input"
|
|
multiple: false
|
|
multiple_sep: ";"
|
|
- type: "file"
|
|
name: "--contaminants"
|
|
alternatives:
|
|
- "-c"
|
|
description: "Specifies a non-default file which contains the list \nof contaminants\
|
|
\ to screen overrepresented sequences against. \nThe file must contain sets\
|
|
\ of named contaminants in the form\nname[tab]sequence. Lines prefixed with\
|
|
\ a hash will be ignored.\n"
|
|
info: null
|
|
example:
|
|
- "contaminants.txt"
|
|
must_exist: true
|
|
create_parent: true
|
|
required: false
|
|
direction: "input"
|
|
multiple: false
|
|
multiple_sep: ";"
|
|
- type: "file"
|
|
name: "--adapters"
|
|
alternatives:
|
|
- "-a"
|
|
description: "Specifies a non-default file which contains the list of \nadapter\
|
|
\ sequences which will be explicitly searched against \nthe library. The file\
|
|
\ must contain sets of named adapters \nin the form name[tab]sequence. Lines\
|
|
\ prefixed with a hash will be ignored.\n"
|
|
info: null
|
|
example:
|
|
- "adapters.txt"
|
|
must_exist: true
|
|
create_parent: true
|
|
required: false
|
|
direction: "input"
|
|
multiple: false
|
|
multiple_sep: ";"
|
|
- type: "file"
|
|
name: "--limits"
|
|
alternatives:
|
|
- "-l"
|
|
description: "Specifies a non-default file which contains \na set of criteria\
|
|
\ which will be used to determine \nthe warn/error limits for the various modules.\
|
|
\ \nThis file can also be used to selectively remove \nsome modules from the\
|
|
\ output altogether. The format \nneeds to mirror the default limits.txt file\
|
|
\ found in \nthe Configuration folder.\n"
|
|
info: null
|
|
example:
|
|
- "limits.txt"
|
|
must_exist: true
|
|
create_parent: true
|
|
required: false
|
|
direction: "input"
|
|
multiple: false
|
|
multiple_sep: ";"
|
|
- type: "integer"
|
|
name: "--kmers"
|
|
alternatives:
|
|
- "-k"
|
|
description: "Specifies the length of Kmer to look for in the Kmer \ncontent module.\
|
|
\ Specified Kmer length must be between \n2 and 10. Default length is 7 if not\
|
|
\ specified.\n"
|
|
info: null
|
|
example:
|
|
- 7
|
|
required: false
|
|
direction: "input"
|
|
multiple: false
|
|
multiple_sep: ";"
|
|
- type: "boolean_true"
|
|
name: "--quiet"
|
|
alternatives:
|
|
- "-q"
|
|
description: "Suppress all progress messages on stdout and only report errors.\n"
|
|
info: null
|
|
direction: "input"
|
|
resources:
|
|
- type: "bash_script"
|
|
path: "script.sh"
|
|
is_executable: true
|
|
description: "FastQC - A high throughput sequence QC analysis tool."
|
|
test_resources:
|
|
- type: "bash_script"
|
|
path: "test.sh"
|
|
is_executable: true
|
|
info: null
|
|
status: "enabled"
|
|
requirements:
|
|
commands:
|
|
- "ps"
|
|
keywords:
|
|
- "Quality control"
|
|
- "BAM"
|
|
- "SAM"
|
|
- "FASTQ"
|
|
license: "GPL-3.0, Apache-2.0"
|
|
links:
|
|
repository: "https://github.com/s-andrews/FastQC"
|
|
homepage: "https://www.bioinformatics.babraham.ac.uk/projects/fastqc/"
|
|
documentation: "https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/"
|
|
issue_tracker: "https://github.com/s-andrews/FastQC/issues"
|
|
runners:
|
|
- type: "executable"
|
|
id: "executable"
|
|
docker_setup_strategy: "ifneedbepullelsecachedbuild"
|
|
- type: "nextflow"
|
|
id: "nextflow"
|
|
directives:
|
|
tag: "$id"
|
|
auto:
|
|
simplifyInput: true
|
|
simplifyOutput: false
|
|
transcript: false
|
|
publish: false
|
|
config:
|
|
labels:
|
|
mem1gb: "memory = 1000000000.B"
|
|
mem2gb: "memory = 2000000000.B"
|
|
mem5gb: "memory = 5000000000.B"
|
|
mem10gb: "memory = 10000000000.B"
|
|
mem20gb: "memory = 20000000000.B"
|
|
mem50gb: "memory = 50000000000.B"
|
|
mem100gb: "memory = 100000000000.B"
|
|
mem200gb: "memory = 200000000000.B"
|
|
mem500gb: "memory = 500000000000.B"
|
|
mem1tb: "memory = 1000000000000.B"
|
|
mem2tb: "memory = 2000000000000.B"
|
|
mem5tb: "memory = 5000000000000.B"
|
|
mem10tb: "memory = 10000000000000.B"
|
|
mem20tb: "memory = 20000000000000.B"
|
|
mem50tb: "memory = 50000000000000.B"
|
|
mem100tb: "memory = 100000000000000.B"
|
|
mem200tb: "memory = 200000000000000.B"
|
|
mem500tb: "memory = 500000000000000.B"
|
|
mem1gib: "memory = 1073741824.B"
|
|
mem2gib: "memory = 2147483648.B"
|
|
mem4gib: "memory = 4294967296.B"
|
|
mem8gib: "memory = 8589934592.B"
|
|
mem16gib: "memory = 17179869184.B"
|
|
mem32gib: "memory = 34359738368.B"
|
|
mem64gib: "memory = 68719476736.B"
|
|
mem128gib: "memory = 137438953472.B"
|
|
mem256gib: "memory = 274877906944.B"
|
|
mem512gib: "memory = 549755813888.B"
|
|
mem1tib: "memory = 1099511627776.B"
|
|
mem2tib: "memory = 2199023255552.B"
|
|
mem4tib: "memory = 4398046511104.B"
|
|
mem8tib: "memory = 8796093022208.B"
|
|
mem16tib: "memory = 17592186044416.B"
|
|
mem32tib: "memory = 35184372088832.B"
|
|
mem64tib: "memory = 70368744177664.B"
|
|
mem128tib: "memory = 140737488355328.B"
|
|
mem256tib: "memory = 281474976710656.B"
|
|
mem512tib: "memory = 562949953421312.B"
|
|
cpu1: "cpus = 1"
|
|
cpu2: "cpus = 2"
|
|
cpu5: "cpus = 5"
|
|
cpu10: "cpus = 10"
|
|
cpu20: "cpus = 20"
|
|
cpu50: "cpus = 50"
|
|
cpu100: "cpus = 100"
|
|
cpu200: "cpus = 200"
|
|
cpu500: "cpus = 500"
|
|
cpu1000: "cpus = 1000"
|
|
debug: false
|
|
container: "docker"
|
|
engines:
|
|
- type: "docker"
|
|
id: "docker"
|
|
image: "biocontainers/fastqc:v0.11.9_cv8"
|
|
target_registry: "images.viash-hub.com"
|
|
target_tag: "main"
|
|
namespace_separator: "/"
|
|
setup:
|
|
- type: "docker"
|
|
run:
|
|
- "echo \"fastqc: $(fastqc --version | sed -n 's/^FastQC //p')\" > /var/software_versions.txt\n"
|
|
entrypoint: []
|
|
cmd: null
|
|
- type: "native"
|
|
id: "native"
|
|
build_info:
|
|
config: "src/fastqc/config.vsh.yaml"
|
|
runner: "executable"
|
|
engine: "docker|native"
|
|
output: "target/executable/fastqc"
|
|
executable: "target/executable/fastqc/fastqc"
|
|
viash_version: "0.9.0"
|
|
git_commit: "7f8bcc2b3e1ffaac9778b6acb42420b19660d1a1"
|
|
git_remote: "https://x-access-token:ghs_aSDBedV4vU66pddFDN6d8UEy0ZQApn08RAsh@github.com/viash-hub/biobox"
|
|
git_tag: "v0.2.0-3-g7f8bcc2"
|
|
package_config:
|
|
name: "biobox"
|
|
version: "main"
|
|
description: "A collection of bioinformatics tools for working with sequence data.\n"
|
|
info: null
|
|
viash_version: "0.9.0"
|
|
source: "src"
|
|
target: "target"
|
|
config_mods:
|
|
- ".requirements.commands := ['ps']\n"
|
|
- ".engines += { type: \"native\" }"
|
|
- ".engines[.type == 'docker'].target_registry := 'images.viash-hub.com'"
|
|
- ".engines[.type == 'docker'].target_tag := 'main'"
|
|
keywords:
|
|
- "bioinformatics"
|
|
- "modules"
|
|
- "sequencing"
|
|
license: "MIT"
|
|
organization: "vsh"
|
|
links:
|
|
repository: "https://github.com/viash-hub/biobox"
|
|
issue_tracker: "https://github.com/viash-hub/biobox/issues"
|