diff --git a/README.qmd b/README.qmd index eb6b5a6..44e5606 100644 --- a/README.qmd +++ b/README.qmd @@ -53,7 +53,7 @@ Two variants of the same workflow are provided, depending on the flexibility in ### Test data -We have provided test data at `gs://viash-hub-test-data/demultiplex/v3/demultiplex_htrnaseq_meta/SingleCell-RNA_P3_2` (Illumina), but please feel free to bring your own. The URL of the test data can be provided as-is to the workflow, or you can download everything and specify a local path. +We have provided test data at `gs://viash-hub-resources/demultiplex/v3/demultiplex_htrnaseq_meta/SingleCell-RNA_P3_2` (Illumina), but please feel free to bring your own. The URL of the test data can be provided as-is to the workflow, or you can download everything and specify a local path. The input data should follow the structure of either Illumina or Element Biosciences sequencers. The workflow will automatically detect which demultiplexer to use (`bclconvert` or `bases2fastq`) based on the presence of either `SampleSheet.csv` or `RunParameters.xml` in the input directory. Demultiplexer can also be set explicitly using the `--demultiplexer` parameter. @@ -79,7 +79,7 @@ Press the ‘Launch’ button and follow the instructions. 3. In the next step, we provide the paramters as follows and leave the rest as defalut: - `input`: - `gs://viash-hub-test-data/demultiplex/v3/demultiplex_htrnaseq_meta/SingleCell-RNA_P3_2` + `gs://viash-hub-resources/demultiplex/v3/demultiplex_htrnaseq_meta/SingleCell-RNA_P3_2` ![](assets/demultiplex-launch-parameters-2.png) @@ -138,8 +138,8 @@ easiest is to use the integrated help functionality, for instance using the following: ``` bash - nextflow run https://packages.viash-hub.com/vsh/demultiplex.git \ - -revision v0.3.4 \ + nextflow run vsh/demultiplex \ + -revision v0.3.9 \ -main-script target/nextflow/workflows/runner/main.nf \ --help ``` @@ -150,11 +150,12 @@ Having this project available locally, you can run the following command: nextflow run vsh/demultiplex \ -r v0.3.9 \ -main-script target/nextflow/runner/main.nf \ ---input "gs://viash-hub-test-data/demultiplex/v3/demultiplex_htrnaseq_meta/SingleCell-RNA_P3_2" \ +--input "gs://viash-hub-resources/demultiplex/v3/demultiplex_htrnaseq_meta/SingleCell-RNA_P3_2" \ --demultiplexer bclconvert \ +--skip_copycomplete_check \ --publish_dir example_output/ \ -profile docker \ --c labels.config +-c src/config/labels.config ``` ### (Optional) Resource usage tuning diff --git a/target/executable/io/interop_summary_to_csv/.config.vsh.yaml b/target/executable/io/interop_summary_to_csv/.config.vsh.yaml index 6cb1a4b..bf82918 100644 --- a/target/executable/io/interop_summary_to_csv/.config.vsh.yaml +++ b/target/executable/io/interop_summary_to_csv/.config.vsh.yaml @@ -151,9 +151,9 @@ build_info: output: "target/executable/io/interop_summary_to_csv" executable: "target/executable/io/interop_summary_to_csv/interop_summary_to_csv" viash_version: "0.9.4" - git_commit: "d755985664a702ccc854d0cb3caa1f86166b669b" + git_commit: "b963aed523a223ab3571d3c449901766c632811d" git_remote: "https://github.com/viash-hub/demultiplex" - git_tag: "v0.1.1-28-gd755985" + git_tag: "v0.1.1-29-gb963aed" package_config: name: "demultiplex" version: "update-readme" diff --git a/target/executable/io/interop_summary_to_csv/interop_summary_to_csv b/target/executable/io/interop_summary_to_csv/interop_summary_to_csv index fa7343f..8e3591e 100755 --- a/target/executable/io/interop_summary_to_csv/interop_summary_to_csv +++ b/target/executable/io/interop_summary_to_csv/interop_summary_to_csv @@ -454,9 +454,9 @@ tar -C /tmp/ --no-same-owner --no-same-permissions -xvf /tmp/interop.tar.gz && \ mv /tmp/interop-1.3.1-Linux-GNU/bin/index-summary /tmp/interop-1.3.1-Linux-GNU/bin/summary /usr/local/bin/ LABEL org.opencontainers.image.description="Companion container for running component io interop_summary_to_csv" -LABEL org.opencontainers.image.created="2025-05-06T07:42:19Z" +LABEL org.opencontainers.image.created="2025-05-06T09:10:44Z" LABEL org.opencontainers.image.source="https://github.com/viash-hub/demultiplex" -LABEL org.opencontainers.image.revision="d755985664a702ccc854d0cb3caa1f86166b669b" +LABEL org.opencontainers.image.revision="b963aed523a223ab3571d3c449901766c632811d" LABEL org.opencontainers.image.version="update-readme" VIASHDOCKER diff --git a/target/executable/io/publish/.config.vsh.yaml b/target/executable/io/publish/.config.vsh.yaml index 0d60af1..38f55f7 100644 --- a/target/executable/io/publish/.config.vsh.yaml +++ b/target/executable/io/publish/.config.vsh.yaml @@ -199,9 +199,9 @@ build_info: output: "target/executable/io/publish" executable: "target/executable/io/publish/publish" viash_version: "0.9.4" - git_commit: "d755985664a702ccc854d0cb3caa1f86166b669b" + git_commit: "b963aed523a223ab3571d3c449901766c632811d" git_remote: "https://github.com/viash-hub/demultiplex" - git_tag: "v0.1.1-28-gd755985" + git_tag: "v0.1.1-29-gb963aed" package_config: name: "demultiplex" version: "update-readme" diff --git a/target/executable/io/publish/publish b/target/executable/io/publish/publish index 926d52a..dd585f9 100755 --- a/target/executable/io/publish/publish +++ b/target/executable/io/publish/publish @@ -450,9 +450,9 @@ RUN apt-get update && \ rm -rf /var/lib/apt/lists/* LABEL org.opencontainers.image.description="Companion container for running component io publish" -LABEL org.opencontainers.image.created="2025-05-06T07:42:19Z" +LABEL org.opencontainers.image.created="2025-05-06T09:10:44Z" LABEL org.opencontainers.image.source="https://github.com/viash-hub/demultiplex" -LABEL org.opencontainers.image.revision="d755985664a702ccc854d0cb3caa1f86166b669b" +LABEL org.opencontainers.image.revision="b963aed523a223ab3571d3c449901766c632811d" LABEL org.opencontainers.image.version="update-readme" VIASHDOCKER diff --git a/target/executable/io/untar/.config.vsh.yaml b/target/executable/io/untar/.config.vsh.yaml index a58fdf9..b28a21e 100644 --- a/target/executable/io/untar/.config.vsh.yaml +++ b/target/executable/io/untar/.config.vsh.yaml @@ -156,9 +156,9 @@ build_info: output: "target/executable/io/untar" executable: "target/executable/io/untar/untar" viash_version: "0.9.4" - git_commit: "d755985664a702ccc854d0cb3caa1f86166b669b" + git_commit: "b963aed523a223ab3571d3c449901766c632811d" git_remote: "https://github.com/viash-hub/demultiplex" - git_tag: "v0.1.1-28-gd755985" + git_tag: "v0.1.1-29-gb963aed" package_config: name: "demultiplex" version: "update-readme" diff --git a/target/executable/io/untar/untar b/target/executable/io/untar/untar index 13347d3..c650d51 100755 --- a/target/executable/io/untar/untar +++ b/target/executable/io/untar/untar @@ -450,9 +450,9 @@ RUN apt-get update && \ rm -rf /var/lib/apt/lists/* LABEL org.opencontainers.image.description="Companion container for running component io untar" -LABEL org.opencontainers.image.created="2025-05-06T07:42:19Z" +LABEL org.opencontainers.image.created="2025-05-06T09:10:44Z" LABEL org.opencontainers.image.source="https://github.com/viash-hub/demultiplex" -LABEL org.opencontainers.image.revision="d755985664a702ccc854d0cb3caa1f86166b669b" +LABEL org.opencontainers.image.revision="b963aed523a223ab3571d3c449901766c632811d" LABEL org.opencontainers.image.version="update-readme" VIASHDOCKER diff --git a/target/nextflow/dataflow/combine_samples/.config.vsh.yaml b/target/nextflow/dataflow/combine_samples/.config.vsh.yaml index dce5699..dc3a45a 100644 --- a/target/nextflow/dataflow/combine_samples/.config.vsh.yaml +++ b/target/nextflow/dataflow/combine_samples/.config.vsh.yaml @@ -165,9 +165,9 @@ build_info: output: "target/nextflow/dataflow/combine_samples" executable: "target/nextflow/dataflow/combine_samples/main.nf" viash_version: "0.9.4" - git_commit: "d755985664a702ccc854d0cb3caa1f86166b669b" + git_commit: "b963aed523a223ab3571d3c449901766c632811d" git_remote: "https://github.com/viash-hub/demultiplex" - git_tag: "v0.1.1-28-gd755985" + git_tag: "v0.1.1-29-gb963aed" package_config: name: "demultiplex" version: "update-readme" diff --git a/target/nextflow/dataflow/combine_samples/main.nf b/target/nextflow/dataflow/combine_samples/main.nf index 36258e9..c6c06ee 100644 --- a/target/nextflow/dataflow/combine_samples/main.nf +++ b/target/nextflow/dataflow/combine_samples/main.nf @@ -3230,9 +3230,9 @@ meta = [ "engine" : "native|native", "output" : "target/nextflow/dataflow/combine_samples", "viash_version" : "0.9.4", - "git_commit" : "d755985664a702ccc854d0cb3caa1f86166b669b", + "git_commit" : "b963aed523a223ab3571d3c449901766c632811d", "git_remote" : "https://github.com/viash-hub/demultiplex", - "git_tag" : "v0.1.1-28-gd755985" + "git_tag" : "v0.1.1-29-gb963aed" }, "package_config" : { "name" : "demultiplex", diff --git a/target/nextflow/dataflow/gather_fastqs_and_validate/.config.vsh.yaml b/target/nextflow/dataflow/gather_fastqs_and_validate/.config.vsh.yaml index 26fdcf8..c3d0bf5 100644 --- a/target/nextflow/dataflow/gather_fastqs_and_validate/.config.vsh.yaml +++ b/target/nextflow/dataflow/gather_fastqs_and_validate/.config.vsh.yaml @@ -141,9 +141,9 @@ build_info: output: "target/nextflow/dataflow/gather_fastqs_and_validate" executable: "target/nextflow/dataflow/gather_fastqs_and_validate/main.nf" viash_version: "0.9.4" - git_commit: "d755985664a702ccc854d0cb3caa1f86166b669b" + git_commit: "b963aed523a223ab3571d3c449901766c632811d" git_remote: "https://github.com/viash-hub/demultiplex" - git_tag: "v0.1.1-28-gd755985" + git_tag: "v0.1.1-29-gb963aed" package_config: name: "demultiplex" version: "update-readme" diff --git a/target/nextflow/dataflow/gather_fastqs_and_validate/main.nf b/target/nextflow/dataflow/gather_fastqs_and_validate/main.nf index 469220f..d08dd45 100644 --- a/target/nextflow/dataflow/gather_fastqs_and_validate/main.nf +++ b/target/nextflow/dataflow/gather_fastqs_and_validate/main.nf @@ -3203,9 +3203,9 @@ meta = [ "engine" : "native|native", "output" : "target/nextflow/dataflow/gather_fastqs_and_validate", "viash_version" : "0.9.4", - "git_commit" : "d755985664a702ccc854d0cb3caa1f86166b669b", + "git_commit" : "b963aed523a223ab3571d3c449901766c632811d", "git_remote" : "https://github.com/viash-hub/demultiplex", - "git_tag" : "v0.1.1-28-gd755985" + "git_tag" : "v0.1.1-29-gb963aed" }, "package_config" : { "name" : "demultiplex", diff --git a/target/nextflow/demultiplex/.config.vsh.yaml b/target/nextflow/demultiplex/.config.vsh.yaml index 23e3c0b..4e7c696 100644 --- a/target/nextflow/demultiplex/.config.vsh.yaml +++ b/target/nextflow/demultiplex/.config.vsh.yaml @@ -247,9 +247,9 @@ build_info: output: "target/nextflow/demultiplex" executable: "target/nextflow/demultiplex/main.nf" viash_version: "0.9.4" - git_commit: "d755985664a702ccc854d0cb3caa1f86166b669b" + git_commit: "b963aed523a223ab3571d3c449901766c632811d" git_remote: "https://github.com/viash-hub/demultiplex" - git_tag: "v0.1.1-28-gd755985" + git_tag: "v0.1.1-29-gb963aed" dependencies: - "target/nextflow/io/untar" - "target/nextflow/dataflow/gather_fastqs_and_validate" diff --git a/target/nextflow/demultiplex/main.nf b/target/nextflow/demultiplex/main.nf index 64d19af..22802e0 100644 --- a/target/nextflow/demultiplex/main.nf +++ b/target/nextflow/demultiplex/main.nf @@ -3350,9 +3350,9 @@ meta = [ "engine" : "native|native", "output" : "target/nextflow/demultiplex", "viash_version" : "0.9.4", - "git_commit" : "d755985664a702ccc854d0cb3caa1f86166b669b", + "git_commit" : "b963aed523a223ab3571d3c449901766c632811d", "git_remote" : "https://github.com/viash-hub/demultiplex", - "git_tag" : "v0.1.1-28-gd755985" + "git_tag" : "v0.1.1-29-gb963aed" }, "package_config" : { "name" : "demultiplex", diff --git a/target/nextflow/io/interop_summary_to_csv/.config.vsh.yaml b/target/nextflow/io/interop_summary_to_csv/.config.vsh.yaml index 3881173..c8cd960 100644 --- a/target/nextflow/io/interop_summary_to_csv/.config.vsh.yaml +++ b/target/nextflow/io/interop_summary_to_csv/.config.vsh.yaml @@ -151,9 +151,9 @@ build_info: output: "target/nextflow/io/interop_summary_to_csv" executable: "target/nextflow/io/interop_summary_to_csv/main.nf" viash_version: "0.9.4" - git_commit: "d755985664a702ccc854d0cb3caa1f86166b669b" + git_commit: "b963aed523a223ab3571d3c449901766c632811d" git_remote: "https://github.com/viash-hub/demultiplex" - git_tag: "v0.1.1-28-gd755985" + git_tag: "v0.1.1-29-gb963aed" package_config: name: "demultiplex" version: "update-readme" diff --git a/target/nextflow/io/interop_summary_to_csv/main.nf b/target/nextflow/io/interop_summary_to_csv/main.nf index d9c7957..134329a 100644 --- a/target/nextflow/io/interop_summary_to_csv/main.nf +++ b/target/nextflow/io/interop_summary_to_csv/main.nf @@ -3217,9 +3217,9 @@ meta = [ "engine" : "docker|native", "output" : "target/nextflow/io/interop_summary_to_csv", "viash_version" : "0.9.4", - "git_commit" : "d755985664a702ccc854d0cb3caa1f86166b669b", + "git_commit" : "b963aed523a223ab3571d3c449901766c632811d", "git_remote" : "https://github.com/viash-hub/demultiplex", - "git_tag" : "v0.1.1-28-gd755985" + "git_tag" : "v0.1.1-29-gb963aed" }, "package_config" : { "name" : "demultiplex", diff --git a/target/nextflow/io/publish/.config.vsh.yaml b/target/nextflow/io/publish/.config.vsh.yaml index 6e36d31..95a4662 100644 --- a/target/nextflow/io/publish/.config.vsh.yaml +++ b/target/nextflow/io/publish/.config.vsh.yaml @@ -199,9 +199,9 @@ build_info: output: "target/nextflow/io/publish" executable: "target/nextflow/io/publish/main.nf" viash_version: "0.9.4" - git_commit: "d755985664a702ccc854d0cb3caa1f86166b669b" + git_commit: "b963aed523a223ab3571d3c449901766c632811d" git_remote: "https://github.com/viash-hub/demultiplex" - git_tag: "v0.1.1-28-gd755985" + git_tag: "v0.1.1-29-gb963aed" package_config: name: "demultiplex" version: "update-readme" diff --git a/target/nextflow/io/publish/main.nf b/target/nextflow/io/publish/main.nf index 362e47c..7c79ecf 100644 --- a/target/nextflow/io/publish/main.nf +++ b/target/nextflow/io/publish/main.nf @@ -3274,9 +3274,9 @@ meta = [ "engine" : "docker|native", "output" : "target/nextflow/io/publish", "viash_version" : "0.9.4", - "git_commit" : "d755985664a702ccc854d0cb3caa1f86166b669b", + "git_commit" : "b963aed523a223ab3571d3c449901766c632811d", "git_remote" : "https://github.com/viash-hub/demultiplex", - "git_tag" : "v0.1.1-28-gd755985" + "git_tag" : "v0.1.1-29-gb963aed" }, "package_config" : { "name" : "demultiplex", diff --git a/target/nextflow/io/untar/.config.vsh.yaml b/target/nextflow/io/untar/.config.vsh.yaml index 8cafb3f..bf021cc 100644 --- a/target/nextflow/io/untar/.config.vsh.yaml +++ b/target/nextflow/io/untar/.config.vsh.yaml @@ -156,9 +156,9 @@ build_info: output: "target/nextflow/io/untar" executable: "target/nextflow/io/untar/main.nf" viash_version: "0.9.4" - git_commit: "d755985664a702ccc854d0cb3caa1f86166b669b" + git_commit: "b963aed523a223ab3571d3c449901766c632811d" git_remote: "https://github.com/viash-hub/demultiplex" - git_tag: "v0.1.1-28-gd755985" + git_tag: "v0.1.1-29-gb963aed" package_config: name: "demultiplex" version: "update-readme" diff --git a/target/nextflow/io/untar/main.nf b/target/nextflow/io/untar/main.nf index d382d87..05b9760 100644 --- a/target/nextflow/io/untar/main.nf +++ b/target/nextflow/io/untar/main.nf @@ -3227,9 +3227,9 @@ meta = [ "engine" : "docker|native", "output" : "target/nextflow/io/untar", "viash_version" : "0.9.4", - "git_commit" : "d755985664a702ccc854d0cb3caa1f86166b669b", + "git_commit" : "b963aed523a223ab3571d3c449901766c632811d", "git_remote" : "https://github.com/viash-hub/demultiplex", - "git_tag" : "v0.1.1-28-gd755985" + "git_tag" : "v0.1.1-29-gb963aed" }, "package_config" : { "name" : "demultiplex", diff --git a/target/nextflow/runner/.config.vsh.yaml b/target/nextflow/runner/.config.vsh.yaml index 4e8a8dc..1c001fc 100644 --- a/target/nextflow/runner/.config.vsh.yaml +++ b/target/nextflow/runner/.config.vsh.yaml @@ -195,9 +195,9 @@ build_info: output: "target/nextflow/runner" executable: "target/nextflow/runner/main.nf" viash_version: "0.9.4" - git_commit: "d755985664a702ccc854d0cb3caa1f86166b669b" + git_commit: "b963aed523a223ab3571d3c449901766c632811d" git_remote: "https://github.com/viash-hub/demultiplex" - git_tag: "v0.1.1-28-gd755985" + git_tag: "v0.1.1-29-gb963aed" dependencies: - "target/nextflow/demultiplex" - "target/nextflow/io/publish" diff --git a/target/nextflow/runner/main.nf b/target/nextflow/runner/main.nf index ddc8285..9b3b316 100644 --- a/target/nextflow/runner/main.nf +++ b/target/nextflow/runner/main.nf @@ -3270,9 +3270,9 @@ meta = [ "engine" : "native|native", "output" : "target/nextflow/runner", "viash_version" : "0.9.4", - "git_commit" : "d755985664a702ccc854d0cb3caa1f86166b669b", + "git_commit" : "b963aed523a223ab3571d3c449901766c632811d", "git_remote" : "https://github.com/viash-hub/demultiplex", - "git_tag" : "v0.1.1-28-gd755985" + "git_tag" : "v0.1.1-29-gb963aed" }, "package_config" : { "name" : "demultiplex",