Build branch main with version main (900f5ed)
Build pipeline: viash-hub.htrnaseq.main-2ww7f
Source commit: 900f5ed356
Source message: Runner: allow multiple input directories. (#38)
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CHANGELOG.md
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CHANGELOG.md
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# htrnaseq v0.x.x
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# htrnaseq v0.4.0
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## Breaking changes
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An effort has been made to align the inputs for the `htrnaseq` and the mapping and demultiplexing of the wells, in order
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simplify running these steps as seperate steps (PR #37).
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* Changes to the `parallel_map` component:
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- The `barcode` argument has been renamed to `barcodesFasta` and the provided
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value for this argument must now be single FASTA file instead of a list of barcodes.
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- The filenames for the provided FASTQ files must now conform to the format `{name}_R(1|2).fasta`,
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where `{name}` is the well identifiers. The well identifiers correspond to the headers
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of the FASTA file containing the barcodes (up untill the first whitespace).
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Forward and reverse FASTQ files must still be provided in pairs, meaning that the order of
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files provided to `input_r1` and `input_r2` remains important.
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- The requirement for equal number of barcodes and FASTQ pairs to be provided has been dropped.
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Instead, the barcodes provided with `barcodesFasta` are matched to the input FASTQ files by comparing
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the header of the FASTA records to the file names of the provided FASTQ input files. Each barcode must
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match exactly one FASTQ input pair (forward and reverse reads), but FASTQ files that were not matched to any
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barcode are not processed. Basically, the barcodes fasta can now act as a filter for the FASTQ files to be mapped.
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* The `utils/groupWells` workflow has been removed.
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* `parallel_map_wf` has been removed as its functionality is now incomporated into the `parallel_map` component.
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* The `pool`, `well_id`, `barcode`, `lane`, `pair_end` and `n_wells` output arguments have been dropped from the
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`well_demultiplexing` workflow. This workflow now only outputs a list of demultiplexed FASTQ files.
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* A `well_metadata` workflow has been implemented that extracts the metadata that is no longer output by the `well_demultiplexing`
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workflow from the demultiplexed files and the barcodes FASTA.
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## New functionality
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* Multiple input directories can not be provided. The input reads from these from these directories
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will be joined per barcode before mapping. This is useful when data has been generated using
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multiple sequencing runs in order to increase sequencing depth (PR #38).
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# htrnaseq v0.3.0
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## New functionality
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