name: htrnaseq summary: | A workflow for high-throughput RNA-seq data analyses. description: | This workflow is designed to process high-throughput RNA-seq data, where every well of a microarray plate is a sample. A fasta file provided as input defines the mapping between sample barcodes and wells. The workflow is built in a modular fashion, where most of the base functionality is provided by components from [`biobox`](https://www.viash-hub.com/packages/biobox/latest) supplemented by custom base components and workflow components in this package. The full workflow is split in two major subworkflows that can be run independently: * **Well-demultiplexing:** Split the input (plate/pool level) fastq files per well. * **Mapping, counting and QC:** Run per-well mapping, counting and generate QC reports. Each of those can be started individually, or the full workflow can be run in two ways: 1. Run the [main workflow](https://www.viash-hub.com/packages/htrnaseq/v0.3.0/components/workflows/htrnaseq) containing the main functionality. 2. Run the [(opinionated) `runner`](https://www.viash-hub.com/packages/htrnaseq/v0.3.0/components/workflows/runner) where a number of choices (input/output structure and location) have been made. Input for the workflow has to be `fastq` files (zipped or not). For bcl or other formats, please consider running [demultiplex](https://www.viash-hub.com/packages/demultiplex) first. license: MIT keywords: [ bioinformatics, sequencing, high-throughput, RNAseq, mapping, counting, pipeline, workflow, ] links: issue_tracker: https://github.com/viash-hub/htrnaseq/issues repository: https://github.com/viash-hub/htrnaseq viash_version: 0.9.4 info: test_resources: - path: gs://viash-hub-resources/htrnaseq/v1 dest: resources_test config_mods: | .requirements.commands := ['ps'] .runners[.type == 'nextflow'].config.script := 'includeConfig("nextflow_labels.config")' .resources += {path: '/src/config/labels.config', dest: 'nextflow_labels.config'}