name: "from_cellranger_multi_to_h5mu" namespace: "convert" version: "dev" authors: - name: "Dries Schaumont" roles: - "maintainer" info: role: "Core Team Member" links: email: "dries@data-intuitive.com" github: "DriesSchaumont" orcid: "0000-0002-4389-0440" linkedin: "dries-schaumont" organizations: - name: "Data Intuitive" href: "https://www.data-intuitive.com" role: "Data Scientist" argument_groups: - name: "Arguments" arguments: - type: "file" name: "--input" alternatives: - "-i" description: "Input folder. Must contain the output from a cellranger multi run." info: null example: - "input_dir_containing_modalities" must_exist: true create_parent: true required: true direction: "input" multiple: false multiple_sep: ";" - type: "file" name: "--output" alternatives: - "-o" description: "Locations for the output files. Must contain a wildcard (*) character,\n\ which will be replaced with the sample name.\n" info: null example: - "*.h5mu" must_exist: true create_parent: true required: false direction: "output" multiple: true multiple_sep: ";" - type: "file" name: "--sample_csv" description: "CSV file describing the sample name per output file" info: null example: - "samples.csv" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" - type: "string" name: "--output_compression" info: null example: - "gzip" required: false choices: - "gzip" - "lzf" direction: "input" multiple: false multiple_sep: ";" - type: "string" name: "--uns_metrics" description: "Name of the .uns slot under which to QC metrics (if any)." info: null default: - "metrics_cellranger" required: false direction: "input" multiple: false multiple_sep: ";" resources: - type: "python_script" path: "script.py" is_executable: true - type: "file" path: "setup_logger.py" - type: "file" path: "nextflow_labels.config" dest: "nextflow_labels.config" description: "Converts the output from cellranger multi to a single .h5mu file.\n\ By default, will map the following library type names to modality names:\n - Gene\ \ Expression: rna\n - Peaks: atac\n - Antibody Capture: prot\n - VDJ: vdj\n \ \ - VDJ-T: vdj_t\n - VDJ-B: vdj_b\n - CRISPR Guide Capture: crispr\n - Multiplexing\ \ Capture: hashing\n \nOther library types have their whitepace removed and dashes\ \ replaced by\nunderscores to generate the modality name.\n\nCurrently does not\ \ allow parsing the output from cell barcode demultiplexing.\n" test_resources: - type: "python_script" path: "test.py" is_executable: true - type: "file" path: "10x_5k_anticmv" - type: "file" path: "10x_5k_lung_crispr" - type: "file" path: "10x_5k_beam" - type: "file" path: "10x_5k_fixed" - type: "file" path: "openpipelinetestutils" dest: "openpipelinetestutils" info: null status: "enabled" links: repository: "https://github.com/openpipelines-bio/openpipeline" docker_registry: "ghcr.io" runners: - type: "executable" id: "executable" docker_setup_strategy: "ifneedbepullelsecachedbuild" - type: "nextflow" id: "nextflow" directives: label: - "lowmem" - "singlecpu" tag: "$id" auto: simplifyInput: true simplifyOutput: false transcript: false publish: false config: labels: mem1gb: "memory = 1000000000.B" mem2gb: "memory = 2000000000.B" mem5gb: "memory = 5000000000.B" mem10gb: "memory = 10000000000.B" mem20gb: "memory = 20000000000.B" mem50gb: "memory = 50000000000.B" mem100gb: "memory = 100000000000.B" mem200gb: "memory = 200000000000.B" mem500gb: "memory = 500000000000.B" mem1tb: "memory = 1000000000000.B" mem2tb: "memory = 2000000000000.B" mem5tb: "memory = 5000000000000.B" mem10tb: "memory = 10000000000000.B" mem20tb: "memory = 20000000000000.B" mem50tb: "memory = 50000000000000.B" mem100tb: "memory = 100000000000000.B" mem200tb: "memory = 200000000000000.B" mem500tb: "memory = 500000000000000.B" mem1gib: "memory = 1073741824.B" mem2gib: "memory = 2147483648.B" mem4gib: "memory = 4294967296.B" mem8gib: "memory = 8589934592.B" mem16gib: "memory = 17179869184.B" mem32gib: "memory = 34359738368.B" mem64gib: "memory = 68719476736.B" mem128gib: "memory = 137438953472.B" mem256gib: "memory = 274877906944.B" mem512gib: "memory = 549755813888.B" mem1tib: "memory = 1099511627776.B" mem2tib: "memory = 2199023255552.B" mem4tib: "memory = 4398046511104.B" mem8tib: "memory = 8796093022208.B" mem16tib: "memory = 17592186044416.B" mem32tib: "memory = 35184372088832.B" mem64tib: "memory = 70368744177664.B" mem128tib: "memory = 140737488355328.B" mem256tib: "memory = 281474976710656.B" mem512tib: "memory = 562949953421312.B" cpu1: "cpus = 1" cpu2: "cpus = 2" cpu5: "cpus = 5" cpu10: "cpus = 10" cpu20: "cpus = 20" cpu50: "cpus = 50" cpu100: "cpus = 100" cpu200: "cpus = 200" cpu500: "cpus = 500" cpu1000: "cpus = 1000" script: - "includeConfig(\"nextflow_labels.config\")" debug: false container: "docker" engines: - type: "docker" id: "docker" image: "python:3.11-slim" target_registry: "images.viash-hub.com" target_tag: "dev" namespace_separator: "/" setup: - type: "apt" packages: - "procps" interactive: false - type: "python" user: false packages: - "anndata==0.10.8" - "mudata~=0.2.4" - "pandas!=2.1.2" - "numpy<2.0.0" - "scanpy~=1.9.6" - "scirpy~=0.12.0" - "pandas~=2.2.2" upgrade: true test_setup: - type: "python" user: false packages: - "viashpy==0.8.0" upgrade: true entrypoint: [] cmd: null - type: "native" id: "native" build_info: config: "src/convert/from_cellranger_multi_to_h5mu/config.vsh.yaml" runner: "executable" engine: "docker|native" output: "target/executable/convert/from_cellranger_multi_to_h5mu" executable: "target/executable/convert/from_cellranger_multi_to_h5mu/from_cellranger_multi_to_h5mu" viash_version: "0.9.0" git_commit: "2dbe3b7231f9abb4baa628e76e8abc686e627087" git_remote: "https://x-access-token:ghs_NVsRTpmVPn6SfFQ131njHQOgn6tt7b1bmmJj@github.com/openpipelines-bio/openpipeline" git_tag: "0.2.0-1926-g2dbe3b72" package_config: name: "openpipeline" version: "dev" info: test_resources: - type: "s3" path: "s3://openpipelines-data" dest: "resources_test" viash_version: "0.9.0" source: "src" target: "target" config_mods: - ".test_resources += {path: '/src/base/openpipelinetestutils', dest: 'openpipelinetestutils'}\n\ .resources += {path: '/src/workflows/utils/labels.config', dest: 'nextflow_labels.config'}\n\ .runners[.type == 'nextflow'].directives.tag := '$id'\n.runners[.type == 'nextflow'].config.script\ \ := 'includeConfig(\"nextflow_labels.config\")'" - ".engines += { type: \"native\" }" - ".engines[.type == 'docker'].target_registry := 'images.viash-hub.com'" - ".engines[.type == 'docker'].target_tag := 'dev'" organization: "vsh" links: repository: "https://github.com/openpipelines-bio/openpipeline" docker_registry: "ghcr.io" homepage: "https://openpipelines.bio" documentation: "https://openpipelines.bio/fundamentals" issue_tracker: "https://github.com/openpipelines-bio/openpipeline/issues"