name: "prot_multisample"
namespace: "workflows/prot"
version: "3.0.0"
authors:
- name: "Dries Schaumont"
  roles:
  - "author"
  info:
    role: "Core Team Member"
    links:
      email: "dries@data-intuitive.com"
      github: "DriesSchaumont"
      orcid: "0000-0002-4389-0440"
      linkedin: "dries-schaumont"
    organizations:
    - name: "Data Intuitive"
      href: "https://www.data-intuitive.com"
      role: "Data Scientist"
argument_groups:
- name: "Inputs"
  arguments:
  - type: "string"
    name: "--id"
    description: "ID of the concatenated file"
    info: null
    example:
    - "concatenated"
    required: true
    direction: "input"
    multiple: false
    multiple_sep: ";"
  - type: "file"
    name: "--input"
    description: "Path to the samples."
    info: null
    example:
    - "dataset.h5mu"
    must_exist: true
    create_parent: true
    required: true
    direction: "input"
    multiple: false
    multiple_sep: ";"
  - type: "string"
    name: "--layer"
    description: "Input layer to use. If not specified, .X is used."
    info: null
    required: false
    direction: "input"
    multiple: false
    multiple_sep: ";"
- name: "Outputs"
  arguments:
  - type: "file"
    name: "--output"
    description: "Destination path to the output."
    info: null
    example:
    - "output.h5mu"
    must_exist: true
    create_parent: true
    required: true
    direction: "output"
    multiple: false
    multiple_sep: ";"
- name: "QC metrics calculation options"
  arguments:
  - type: "string"
    name: "--var_qc_metrics"
    description: "Keys to select a boolean (containing only True or False) column\
      \ from .var.\nFor each cell, calculate the proportion of total values for genes\
      \ which are labeled 'True', \ncompared to the total sum of the values for all\
      \ genes. Defaults to the value from\n--var_name_mitochondrial_genes.\n"
    info: null
    example:
    - "ercc,highly_variable"
    required: false
    direction: "input"
    multiple: true
    multiple_sep: ","
  - type: "integer"
    name: "--top_n_vars"
    description: "Number of top vars to be used to calculate cumulative proportions.\n\
      If not specified, proportions are not calculated. `--top_n_vars 20,50` finds\n\
      cumulative proportion to the 20th and 50th most expressed vars.\n"
    info: null
    default:
    - 50
    - 100
    - 200
    - 500
    required: false
    direction: "input"
    multiple: true
    multiple_sep: ","
  - type: "string"
    name: "--output_obs_num_nonzero_vars"
    description: "Name of column in .obs describing, for each observation, the number\
      \ of stored values\n(including explicit zeroes). In other words, the name of\
      \ the column that counts\nfor each row the number of columns that contain data.\n"
    info: null
    default:
    - "num_nonzero_vars"
    required: false
    direction: "input"
    multiple: false
    multiple_sep: ";"
  - type: "string"
    name: "--output_obs_total_counts_vars"
    description: "Name of the column for .obs describing, for each observation (row),\n\
      the sum of the stored values in the columns.\n"
    info: null
    default:
    - "total_counts"
    required: false
    direction: "input"
    multiple: false
    multiple_sep: ";"
  - type: "string"
    name: "--output_var_num_nonzero_obs"
    description: "Name of column describing, for each feature, the number of stored\
      \ values\n(including explicit zeroes). In other words, the name of the column\
      \ that counts\nfor each column the number of rows that contain data.\n"
    info: null
    default:
    - "num_nonzero_obs"
    required: false
    direction: "input"
    multiple: false
    multiple_sep: ";"
  - type: "string"
    name: "--output_var_total_counts_obs"
    description: "Name of the column in .var describing, for each feature (column),\n\
      the sum of the stored values in the rows.\n"
    info: null
    default:
    - "total_counts"
    required: false
    direction: "input"
    multiple: false
    multiple_sep: ";"
  - type: "string"
    name: "--output_var_obs_mean"
    description: "Name of the column in .obs providing the mean of the values in each\
      \ row.\n"
    info: null
    default:
    - "obs_mean"
    required: false
    direction: "input"
    multiple: false
    multiple_sep: ";"
  - type: "string"
    name: "--output_var_pct_dropout"
    description: "Name of the column in .obs providing for each feature the percentage\
      \ of\nobservations the feature does not appear on (i.e. is missing). Same as\
      \ `--output_var_num_nonzero_obs`\nbut percentage based.\n"
    info: null
    default:
    - "pct_dropout"
    required: false
    direction: "input"
    multiple: false
    multiple_sep: ";"
- name: "CLR arguments"
  arguments:
  - type: "integer"
    name: "--clr_axis"
    description: "Axis across which CLR is performed."
    info: null
    default:
    - 0
    required: false
    direction: "input"
    multiple: false
    multiple_sep: ";"
resources:
- type: "nextflow_script"
  path: "main.nf"
  is_executable: true
  entrypoint: "run_wf"
- type: "file"
  path: "utils"
- type: "file"
  path: "nextflow_labels.config"
  dest: "nextflow_labels.config"
description: "Processing unimodal multi-sample ADT data."
test_resources:
- type: "nextflow_script"
  path: "test.nf"
  is_executable: true
  entrypoint: "test_wf"
- type: "file"
  path: "pbmc_1k_protein_v3"
info:
  image: "/images/concepts/fig_workflow_multiomics_adt_multisample.svg"
status: "enabled"
scope:
  image: "public"
  target: "public"
dependencies:
- name: "transform/clr"
  repository:
    type: "local"
- name: "workflows/qc/qc"
  alias: "prot_qc"
  repository:
    type: "local"
license: "MIT"
links:
  repository: "https://github.com/openpipelines-bio/openpipeline"
  docker_registry: "ghcr.io"
runners:
- type: "nextflow"
  id: "nextflow"
  directives:
    tag: "$id"
  auto:
    simplifyInput: true
    simplifyOutput: false
    transcript: false
    publish: false
  config:
    labels:
      mem1gb: "memory = 1000000000.B"
      mem2gb: "memory = 2000000000.B"
      mem5gb: "memory = 5000000000.B"
      mem10gb: "memory = 10000000000.B"
      mem20gb: "memory = 20000000000.B"
      mem50gb: "memory = 50000000000.B"
      mem100gb: "memory = 100000000000.B"
      mem200gb: "memory = 200000000000.B"
      mem500gb: "memory = 500000000000.B"
      mem1tb: "memory = 1000000000000.B"
      mem2tb: "memory = 2000000000000.B"
      mem5tb: "memory = 5000000000000.B"
      mem10tb: "memory = 10000000000000.B"
      mem20tb: "memory = 20000000000000.B"
      mem50tb: "memory = 50000000000000.B"
      mem100tb: "memory = 100000000000000.B"
      mem200tb: "memory = 200000000000000.B"
      mem500tb: "memory = 500000000000000.B"
      mem1gib: "memory = 1073741824.B"
      mem2gib: "memory = 2147483648.B"
      mem4gib: "memory = 4294967296.B"
      mem8gib: "memory = 8589934592.B"
      mem16gib: "memory = 17179869184.B"
      mem32gib: "memory = 34359738368.B"
      mem64gib: "memory = 68719476736.B"
      mem128gib: "memory = 137438953472.B"
      mem256gib: "memory = 274877906944.B"
      mem512gib: "memory = 549755813888.B"
      mem1tib: "memory = 1099511627776.B"
      mem2tib: "memory = 2199023255552.B"
      mem4tib: "memory = 4398046511104.B"
      mem8tib: "memory = 8796093022208.B"
      mem16tib: "memory = 17592186044416.B"
      mem32tib: "memory = 35184372088832.B"
      mem64tib: "memory = 70368744177664.B"
      mem128tib: "memory = 140737488355328.B"
      mem256tib: "memory = 281474976710656.B"
      mem512tib: "memory = 562949953421312.B"
      cpu1: "cpus = 1"
      cpu2: "cpus = 2"
      cpu5: "cpus = 5"
      cpu10: "cpus = 10"
      cpu20: "cpus = 20"
      cpu50: "cpus = 50"
      cpu100: "cpus = 100"
      cpu200: "cpus = 200"
      cpu500: "cpus = 500"
      cpu1000: "cpus = 1000"
    script:
    - "includeConfig(\"nextflow_labels.config\")"
  debug: false
  container: "docker"
build_info:
  config: "src/workflows/prot/prot_multisample/config.vsh.yaml"
  runner: "nextflow"
  engine: "native"
  output: "target/nextflow/workflows/prot/prot_multisample"
  executable: "target/nextflow/workflows/prot/prot_multisample/main.nf"
  viash_version: "0.9.4"
  git_commit: "706b5ce24d313dcf947b7d9fe929630f1ad204e7"
  git_remote: "https://github.com/openpipelines-bio/openpipeline"
  git_tag: "2.1.0-2-g706b5ce24d3"
  dependencies:
  - "target/nextflow/transform/clr"
  - "target/nextflow/workflows/qc/qc"
package_config:
  name: "openpipeline"
  summary: "Best-practice workflows for single-cell multi-omics analyses.\n"
  description: "OpenPipelines are extensible single cell analysis pipelines for reproducible\
    \ and large-scale single cell processing using [Viash](https://viash.io) and [Nextflow](https://www.nextflow.io/).\n\
    \nIn terms of workflows, the following has been made available, but keep in mind\
    \ that\nindividual tools and functionality can be executed as standalone components\
    \ as well.\n\n  * Demultiplexing: conversion of raw sequencing data to FASTQ objects.\n\
    \  * Ingestion: Read mapping and generating a count matrix.\n  * Single sample\
    \ processing: cell filtering and doublet detection.\n  * Multisample processing:\
    \ Count transformation, normalization, QC metric calulations.\n  * Integration:\
    \ Clustering, integration and batch correction using single and multimodal methods.\n\
    \  * Downstream analysis workflows\n"
  info:
    test_resources:
    - type: "s3"
      path: "s3://openpipelines-data"
      dest: "resources_test"
    nextflow_labels_ci:
    - path: "src/workflows/utils/labels_ci.config"
      description: "Adds the correct memory and CPU labels when running on the Viash\
        \ Hub CI."
  viash_version: "0.9.4"
  source: "src"
  target: "target"
  config_mods:
  - ".resources += {path: '/src/workflows/utils/labels.config', dest: 'nextflow_labels.config'}\n\
    .runners[.type == 'nextflow'].config.script := 'includeConfig(\"nextflow_labels.config\"\
    )'"
  - ".version := \"3.0.0\""
  keywords:
  - "single-cell"
  - "multimodal"
  license: "MIT"
  organization: "openpipelines-bio"
  links:
    repository: "https://github.com/openpipelines-bio/openpipeline"
    docker_registry: "ghcr.io"
    homepage: "https://openpipelines.bio"
    documentation: "https://openpipelines.bio/fundamentals"
    issue_tracker: "https://github.com/openpipelines-bio/openpipeline/issues"
