name: "from_cosmx_to_spatialexperiment" namespace: "convert" version: "build_main" authors: - name: "Dorien Roosen" roles: - "author" - "maintainer" info: role: "Core Team Member" links: email: "dorien@data-intuitive.com" github: "dorien-er" linkedin: "dorien-roosen" organizations: - name: "Data Intuitive" href: "https://www.data-intuitive.com" role: "Data Scientist" argument_groups: - name: "Arguments" arguments: - type: "file" name: "--input" alternatives: - "-i" description: "Input CosMx directory" info: null example: - "path/to/cosmx_bundle" must_exist: true create_parent: true required: true direction: "input" multiple: false multiple_sep: ";" - type: "boolean" name: "--add_tx_path" description: "Whether to add parquet paths to the metadata. \nIf True, `*_tx_file.csv`\ \ file will be converted to .parquet and added to the metadata. \n" info: null default: - true required: false direction: "input" multiple: false multiple_sep: ";" - type: "boolean" name: "--add_polygon_path" description: "Whether to add polygon path to the metadata.\nIf True, `*_polygons.csv`\ \ file will be converted to .parquet and be added to the metadata.\n" info: null default: - true required: false direction: "input" multiple: false multiple_sep: ";" - type: "boolean" name: "--add_fov_positions" description: "Whether to add fov positions to the metadata.\nIf True, `fov_positions_file.csv`\ \ will be added to the metadata.\n" info: null default: - true required: false direction: "input" multiple: false multiple_sep: ";" - type: "string" name: "--alternative_experiment_features" description: "Feature names containing these strings will be moved to altExps(sxe)\ \ slots as separate SpatialExperiment objects." info: null default: - "NegPrb" - "Negative" - "SystemControl" - "FalseCode" required: false direction: "input" multiple: true multiple_sep: ";" - type: "file" name: "--output" alternatives: - "-o" description: "Output SpatialExperiment file" info: null example: - "output.rds" must_exist: true create_parent: true required: true direction: "output" multiple: false multiple_sep: ";" resources: - type: "r_script" path: "script.R" is_executable: true - type: "file" path: "nextflow_labels.config" dest: "nextflow_labels.config" description: "Creates a SpatialExperiment object from the downloaded unzipped CosMx\ \ directory for Nanostring\nCosMx spatial gene expression data, and saves it as\ \ a SpatialExperiment object.\nThe constructor assumes the downloaded unzipped CosMx\ \ Folder has the following structure:\n\nMandatory files\n· | — *_exprMat_file.csv\n\ · | — *_metadata_file.csv\nOptional files, by default added to the metadata() as\ \ a list of paths (will be converted to parquet):\n· | —*_fov_positions_file.csv\n\ · | — *_tx_file.csv\n· | — *_polygons.csv\n" test_resources: - type: "r_script" path: "test.R" is_executable: true - type: "file" path: "Lung5_Rep2_tiny" info: null status: "enabled" scope: image: "public" target: "public" repositories: - type: "github" name: "openpipeline" repo: "openpipelines-bio/openpipeline" tag: "2.1.2" links: repository: "https://github.com/openpipelines-bio/openpipeline_spatial" docker_registry: "ghcr.io" runners: - type: "executable" id: "executable" docker_setup_strategy: "ifneedbepullelsecachedbuild" - type: "nextflow" id: "nextflow" directives: label: - "lowmem" - "singlecpu" tag: "$id" auto: simplifyInput: true simplifyOutput: false transcript: false publish: false config: labels: mem1gb: "memory = 1000000000.B" mem2gb: "memory = 2000000000.B" mem5gb: "memory = 5000000000.B" mem10gb: "memory = 10000000000.B" mem20gb: "memory = 20000000000.B" mem50gb: "memory = 50000000000.B" mem100gb: "memory = 100000000000.B" mem200gb: "memory = 200000000000.B" mem500gb: "memory = 500000000000.B" mem1tb: "memory = 1000000000000.B" mem2tb: "memory = 2000000000000.B" mem5tb: "memory = 5000000000000.B" mem10tb: "memory = 10000000000000.B" mem20tb: "memory = 20000000000000.B" mem50tb: "memory = 50000000000000.B" mem100tb: "memory = 100000000000000.B" mem200tb: "memory = 200000000000000.B" mem500tb: "memory = 500000000000000.B" mem1gib: "memory = 1073741824.B" mem2gib: "memory = 2147483648.B" mem4gib: "memory = 4294967296.B" mem8gib: "memory = 8589934592.B" mem16gib: "memory = 17179869184.B" mem32gib: "memory = 34359738368.B" mem64gib: "memory = 68719476736.B" mem128gib: "memory = 137438953472.B" mem256gib: "memory = 274877906944.B" mem512gib: "memory = 549755813888.B" mem1tib: "memory = 1099511627776.B" mem2tib: "memory = 2199023255552.B" mem4tib: "memory = 4398046511104.B" mem8tib: "memory = 8796093022208.B" mem16tib: "memory = 17592186044416.B" mem32tib: "memory = 35184372088832.B" mem64tib: "memory = 70368744177664.B" mem128tib: "memory = 140737488355328.B" mem256tib: "memory = 281474976710656.B" mem512tib: "memory = 562949953421312.B" cpu1: "cpus = 1" cpu2: "cpus = 2" cpu5: "cpus = 5" cpu10: "cpus = 10" cpu20: "cpus = 20" cpu50: "cpus = 50" cpu100: "cpus = 100" cpu200: "cpus = 200" cpu500: "cpus = 500" cpu1000: "cpus = 1000" script: - "includeConfig(\"nextflow_labels.config\")" debug: false container: "docker" engines: - type: "docker" id: "docker" image: "rocker/r2u:24.04" target_registry: "images.viash-hub.com" target_tag: "build_main" namespace_separator: "/" setup: - type: "apt" packages: - "libhdf5-dev" - "libgeos-dev" interactive: false - type: "r" bioc: - "SpatialExperimentIO" bioc_force_install: false warnings_as_errors: true test_setup: - type: "r" cran: - "testthat" bioc_force_install: false warnings_as_errors: true entrypoint: [] cmd: null - type: "native" id: "native" build_info: config: "src/convert/from_cosmx_to_spatialexperiment/config.vsh.yaml" runner: "nextflow" engine: "docker|native" output: "target/nextflow/convert/from_cosmx_to_spatialexperiment" executable: "target/nextflow/convert/from_cosmx_to_spatialexperiment/main.nf" viash_version: "0.9.4" git_commit: "6f308e6a3cca52f1283fbba734a3cdc858e18e1b" git_remote: "https://github.com/openpipelines-bio/openpipeline_spatial" package_config: name: "openpipeline_spatial" version: "build_main" info: test_resources: - type: "s3" path: "s3://openpipelines-bio/openpipeline_spatial/resources_test" dest: "resources_test" repositories: - type: "github" name: "openpipeline" repo: "openpipelines-bio/openpipeline" tag: "2.1.2" viash_version: "0.9.4" source: "src" target: "target" config_mods: - ".resources += {path: '/src/workflows/utils/labels.config', dest: 'nextflow_labels.config'}\n\ .runners[.type == 'nextflow'].config.script := 'includeConfig(\"nextflow_labels.config\"\ )'" - ".engines += { type: \"native\" }" - ".engines[.type == 'docker'].target_registry := 'images.viash-hub.com'" - ".engines[.type == 'docker'].target_tag := 'build_main'" organization: "vsh" links: repository: "https://github.com/openpipelines-bio/openpipeline_spatial" docker_registry: "ghcr.io"