#!/bin/bash set -eo pipefail # get the root of the directory REPO_ROOT=$(git rev-parse --show-toplevel) # ensure that the command below is run from the root of the repository cd "$REPO_ROOT" DIR="resources_test/cosmx" ID="Lung5_Rep2" OUT="$DIR/$ID/" # create tempdir MY_TEMP="${VIASH_TEMP:-/tmp}" TMPDIR=$(mktemp -d "$MY_TEMP/$ID-XXXXXX") function clean_up { [[ -d "$TMPDIR" ]] && rm -r "$TMPDIR" } trap clean_up EXIT if [ ! -d "$OUT" ]; then flat_dataset="https://nanostring-public-share.s3.us-west-2.amazonaws.com/SMI-Compressed/Lung5_Rep2/Lung5_Rep2+SMI+Flat+data.tar.gz" wget "$flat_dataset" -O "$TMPDIR/Lung5_Rep2.tar.gz" mkdir -p "$TMPDIR/Lung5_Rep2" tar -xzf "$TMPDIR/Lung5_Rep2.tar.gz" -C "$TMPDIR/Lung5_Rep2" mkdir -p "$OUT" mv "$TMPDIR/Lung5_Rep2/Lung5_Rep2/Lung5_Rep2-Flat_files_and_images/"* "$OUT/" fi viash run src/filter/subset_cosmx/config.vsh.yaml -- \ --input "$OUT" \ --num_fovs 3 \ --subset_transcripts_file True \ --subset_polygons_file False \ --output "${DIR}/${ID}_tiny" viash run src/convert/from_cosmx_to_h5mu/config.vsh.yaml -- \ --input ${DIR}/${ID}_tiny \ --output "$DIR/${ID}_tiny.h5mu" \ --output_compression "gzip" rm -rf "$OUT" # Sync to S3 aws s3 sync \ --profile di \ "$DIR" \ s3://openpipelines-bio/openpipeline_spatial/resources_test/cosmx \ --delete \ --dryrun