name: "rseqc_junctionannotation" namespace: "rseqc" version: "main" argument_groups: - name: "Input" arguments: - type: "file" name: "--input" description: "input alignment file in BAM or SAM format" info: null must_exist: true create_parent: true required: true direction: "input" multiple: false multiple_sep: ";" - type: "file" name: "--refgene" description: "Reference gene model in bed format" info: null must_exist: true create_parent: true required: true direction: "input" multiple: false multiple_sep: ";" - type: "integer" name: "--map_qual" description: "Minimum mapping quality (phred scaled) to determine uniquely mapped\ \ reads, default=30." info: null default: - 30 required: false min: 0 direction: "input" multiple: false multiple_sep: ";" - type: "integer" name: "--min_intron" description: "Minimum intron length (bp), default = 50." info: null default: - 50 required: false min: 1 direction: "input" multiple: false multiple_sep: ";" - name: "Output" arguments: - type: "file" name: "--output_log" description: "output log of junction annotation script" info: null default: - "$id.junction_annotation.log" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" - type: "file" name: "--output_plot_r" description: "r script to generate splice_junction and splice_events plot" info: null default: - "$id.junction_annotation_plot.r" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" - type: "file" name: "--output_junction_bed" description: "junction annotation file (bed format)" info: null default: - "$id.junction_annotation.bed" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" - type: "file" name: "--output_junction_interact" description: "interact file (bed format) of junctions. Can be uploaded to UCSC\ \ genome browser or converted to bigInteract (using bedToBigBed program) for\ \ visualization." info: null default: - "$id.junction_annotation.Interact.bed" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" - type: "file" name: "--output_junction_sheet" description: "junction annotation file (xls format)" info: null default: - "$id.junction_annotation.xls" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" - type: "file" name: "--output_splice_events_plot" description: "plot of splice events (pdf)" info: null default: - "$id.splice_events.pdf" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" - type: "file" name: "--output_splice_junctions_plot" description: "plot of junctions (pdf)" info: null default: - "$id.splice_junctions_plot.pdf" must_exist: true create_parent: true required: false direction: "output" multiple: false multiple_sep: ";" resources: - type: "bash_script" path: "script.sh" is_executable: true description: "Compare detected splice junctions to reference gene model.\n" test_resources: - type: "bash_script" path: "test.sh" is_executable: true - type: "file" path: "test.paired_end.sorted.bam" - type: "file" path: "test.bed12" info: migration_info: git_repo: "https://github.com/nf-core/rnaseq.git" paths: - "modules/nf-core/rseqc/junctionannotation/main.nf" status: "enabled" requirements: commands: - "ps" repositories: - type: "vsh" name: "biobox" repo: "biobox" tag: "main" - type: "vsh" name: "craftbox" repo: "craftbox" tag: "v0.1.0" runners: - type: "executable" id: "executable" docker_setup_strategy: "ifneedbepullelsecachedbuild" - type: "nextflow" id: "nextflow" directives: tag: "$id" auto: simplifyInput: true simplifyOutput: false transcript: false publish: false config: labels: mem1gb: "memory = 1000000000.B" mem2gb: "memory = 2000000000.B" mem5gb: "memory = 5000000000.B" mem10gb: "memory = 10000000000.B" mem20gb: "memory = 20000000000.B" mem50gb: "memory = 50000000000.B" mem100gb: "memory = 100000000000.B" mem200gb: "memory = 200000000000.B" mem500gb: "memory = 500000000000.B" mem1tb: "memory = 1000000000000.B" mem2tb: "memory = 2000000000000.B" mem5tb: "memory = 5000000000000.B" mem10tb: "memory = 10000000000000.B" mem20tb: "memory = 20000000000000.B" mem50tb: "memory = 50000000000000.B" mem100tb: "memory = 100000000000000.B" mem200tb: "memory = 200000000000000.B" mem500tb: "memory = 500000000000000.B" mem1gib: "memory = 1073741824.B" mem2gib: "memory = 2147483648.B" mem4gib: "memory = 4294967296.B" mem8gib: "memory = 8589934592.B" mem16gib: "memory = 17179869184.B" mem32gib: "memory = 34359738368.B" mem64gib: "memory = 68719476736.B" mem128gib: "memory = 137438953472.B" mem256gib: "memory = 274877906944.B" mem512gib: "memory = 549755813888.B" mem1tib: "memory = 1099511627776.B" mem2tib: "memory = 2199023255552.B" mem4tib: "memory = 4398046511104.B" mem8tib: "memory = 8796093022208.B" mem16tib: "memory = 17592186044416.B" mem32tib: "memory = 35184372088832.B" mem64tib: "memory = 70368744177664.B" mem128tib: "memory = 140737488355328.B" mem256tib: "memory = 281474976710656.B" mem512tib: "memory = 562949953421312.B" cpu1: "cpus = 1" cpu2: "cpus = 2" cpu5: "cpus = 5" cpu10: "cpus = 10" cpu20: "cpus = 20" cpu50: "cpus = 50" cpu100: "cpus = 100" cpu200: "cpus = 200" cpu500: "cpus = 500" cpu1000: "cpus = 1000" debug: false container: "docker" engines: - type: "docker" id: "docker" image: "ubuntu:22.04" target_registry: "images.viash-hub.com" target_tag: "main" namespace_separator: "/" setup: - type: "apt" packages: - "python3-pip" - "r-base" interactive: false - type: "python" user: false packages: - "RSeQC" upgrade: true entrypoint: [] cmd: null - type: "native" id: "native" build_info: config: "src/rseqc/rseqc_junctionannotation/config.vsh.yaml" runner: "executable" engine: "docker|native" output: "target/executable/rseqc/rseqc_junctionannotation" executable: "target/executable/rseqc/rseqc_junctionannotation/rseqc_junctionannotation" viash_version: "0.9.0" git_commit: "d63250ae704b6bd9a11792c4f979be2309999a2e" git_remote: "https://x-access-token:ghs_ri9q8QB7jMaviilgc8bYrtSqMrabrg3q8JZY@github.com/viash-hub/rnaseq" package_config: name: "rnaseq" version: "main" info: test_resources: - path: "gs://viash-hub-test-data/rnaseq/v1" dest: "testData" repositories: - type: "vsh" name: "biobox" repo: "biobox" tag: "main" - type: "vsh" name: "craftbox" repo: "craftbox" tag: "v0.1.0" viash_version: "0.9.0" source: "src" target: "target" config_mods: - ".requirements.commands := ['ps']\n.runners[.type == 'nextflow'].directives.tag\ \ := '$id'\n" - ".engines += { type: \"native\" }" - ".engines[.type == 'docker'].target_registry := 'images.viash-hub.com'" - ".engines[.type == 'docker'].target_tag := 'main'" organization: "vsh"