CI
93ac6aad2e
Build pipeline: viash-hub.rnaseq.main-nn8dl
Source commit: 0c8a7eb648
Source message: remove citation
424 lines
16 KiB
JSON
424 lines
16 KiB
JSON
{
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"$schema": "http://json-schema.org/draft-07/schema",
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"title": "prepare_genome",
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"description": "A subworkflow for preparing all the required genome references\n",
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"type": "object",
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"definitions": {
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"input" : {
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"title": "Input",
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"type": "object",
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"description": "No description",
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"properties": {
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"fasta": {
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"type":
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"string",
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"description": "Type: `file`, required. Path to FASTA genome file",
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"help_text": "Type: `file`, required. Path to FASTA genome file."
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}
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,
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"gtf": {
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"type":
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"string",
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"description": "Type: `file`. Path to GTF annotation file",
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"help_text": "Type: `file`. Path to GTF annotation file. This parameter is *mandatory* if --genome is not specified."
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}
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,
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"gff": {
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"type":
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"string",
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"description": "Type: `file`. Path to GFF3 annotation file",
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"help_text": "Type: `file`. Path to GFF3 annotation file. Required if \"--gtf\" is not specified."
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}
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,
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"additional_fasta": {
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"type":
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"string",
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"description": "Type: `file`. FASTA file to concatenate to genome FASTA file e",
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"help_text": "Type: `file`. FASTA file to concatenate to genome FASTA file e.g. containing spike-in sequences."
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}
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,
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"transcript_fasta": {
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"type":
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"string",
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"description": "Type: `file`. Path to FASTA transcriptome file",
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"help_text": "Type: `file`. Path to FASTA transcriptome file."
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}
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,
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"gene_bed": {
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"type":
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"string",
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"description": "Type: `file`. Path to BED file containing gene intervals",
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"help_text": "Type: `file`. Path to BED file containing gene intervals. This will be created from the GTF file if not specified."
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}
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,
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"splicesites": {
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"type":
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"string",
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"description": "Type: `file`. Splice sites file required for HISAT2",
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"help_text": "Type: `file`. Splice sites file required for HISAT2."
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}
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,
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"skip_bbsplit": {
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"type":
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"boolean",
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"description": "Type: `boolean`. Skip BBSplit for removal of non-reference genome reads",
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"help_text": "Type: `boolean`. Skip BBSplit for removal of non-reference genome reads."
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}
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,
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"bbsplit_fasta_list": {
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"type":
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"string",
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"description": "Type: List of `file`, multiple_sep: `\";\"`. List of reference genomes (separated by \";\") to filter reads against with BBSplit",
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"help_text": "Type: List of `file`, multiple_sep: `\";\"`. List of reference genomes (separated by \";\") to filter reads against with BBSplit."
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}
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,
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"star_index": {
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"type":
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"string",
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"description": "Type: `file`. Path to directory or tar",
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"help_text": "Type: `file`. Path to directory or tar.gz archive for pre-built STAR index."
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}
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,
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"star_sjdb_gtf_feature_exon": {
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"type":
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"string",
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"description": "Type: `string`. Feature type in GTF file to be used as exons for building transcripts",
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"help_text": "Type: `string`. Feature type in GTF file to be used as exons for building transcripts"
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}
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,
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"rsem_index": {
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"type":
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"string",
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"description": "Type: `file`. Path to directory or tar",
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"help_text": "Type: `file`. Path to directory or tar.gz archive for pre-built RSEM index."
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}
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,
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"salmon_index": {
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"type":
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"string",
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"description": "Type: `file`. Path to directory or tar",
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"help_text": "Type: `file`. Path to directory or tar.gz archive for pre-built Salmon index."
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}
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,
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"kallisto_index": {
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"type":
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"string",
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"description": "Type: `file`. Path to directory or tar",
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"help_text": "Type: `file`. Path to directory or tar.gz archive for pre-built Kallisto index."
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}
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,
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"bbsplit_index": {
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"type":
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"string",
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"description": "Type: `file`. Path to directory or tar",
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"help_text": "Type: `file`. Path to directory or tar.gz archive for pre-built BBSplit index."
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}
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,
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"pseudo_aligner_kmer_size": {
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"type":
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"integer",
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"description": "Type: `integer`, default: `31`. Kmer length passed to indexing step of pseudoaligners",
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"help_text": "Type: `integer`, default: `31`. Kmer length passed to indexing step of pseudoaligners."
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,
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"default":31
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}
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,
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"gencode": {
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"type":
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"boolean",
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"description": "Type: `boolean`. Specify if the GTF annotation is in GENCODE format",
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"help_text": "Type: `boolean`. Specify if the GTF annotation is in GENCODE format."
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}
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,
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"biotype": {
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"type":
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"string",
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"description": "Type: `string`. Biotype value to use while appending entries to GTF file when additional fasta file is provided",
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"help_text": "Type: `string`. Biotype value to use while appending entries to GTF file when additional fasta file is provided."
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}
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,
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"filter_gtf": {
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"type":
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"boolean",
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"description": "Type: `boolean`. Whether to filter the GTF or not?",
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"help_text": "Type: `boolean`. Whether to filter the GTF or not?"
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}
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,
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"aligner": {
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"type":
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"string",
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"description": "Type: `string`, default: `star_salmon`, choices: ``star_salmon`, `star_rsem`, `hisat2``. Specifies the alignment algorithm to use - available options are \u0027star_salmon\u0027, \u0027star_rsem\u0027 and \u0027hisat2\u0027",
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"help_text": "Type: `string`, default: `star_salmon`, choices: ``star_salmon`, `star_rsem`, `hisat2``. Specifies the alignment algorithm to use - available options are \u0027star_salmon\u0027, \u0027star_rsem\u0027 and \u0027hisat2\u0027.",
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"enum": ["star_salmon", "star_rsem", "hisat2"]
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,
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"default":"star_salmon"
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}
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,
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"pseudo_aligner": {
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"type":
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"string",
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"description": "Type: `string`, default: `salmon`, choices: ``salmon`, `kallisto``. Specifies the pseudo aligner to use - available options are \u0027salmon\u0027",
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"help_text": "Type: `string`, default: `salmon`, choices: ``salmon`, `kallisto``. Specifies the pseudo aligner to use - available options are \u0027salmon\u0027. Runs in addition to \u0027--aligner\u0027.",
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"enum": ["salmon", "kallisto"]
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,
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"default":"salmon"
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}
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,
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"skip_alignment": {
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"type":
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"boolean",
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"description": "Type: `boolean_true`, default: `false`. Skip all of the alignment-based processes within the pipeline",
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"help_text": "Type: `boolean_true`, default: `false`. Skip all of the alignment-based processes within the pipeline."
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,
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"default":false
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}
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}
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},
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"output" : {
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"title": "Output",
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"type": "object",
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"description": "No description",
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"properties": {
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"fasta_uncompressed": {
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"type":
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"string",
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"description": "Type: `file`, default: `$id.$key.fasta_uncompressed.fasta`. ",
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"help_text": "Type: `file`, default: `$id.$key.fasta_uncompressed.fasta`. "
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,
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"default":"$id.$key.fasta_uncompressed.fasta"
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}
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,
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"gtf_uncompressed": {
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"type":
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"string",
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"description": "Type: `file`, default: `$id.$key.gtf_uncompressed.gtf`. ",
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"help_text": "Type: `file`, default: `$id.$key.gtf_uncompressed.gtf`. "
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,
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"default":"$id.$key.gtf_uncompressed.gtf"
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}
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,
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"transcript_fasta_uncompressed": {
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"type":
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"string",
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"description": "Type: `file`, default: `$id.$key.transcript_fasta_uncompressed.fasta`. ",
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"help_text": "Type: `file`, default: `$id.$key.transcript_fasta_uncompressed.fasta`. "
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,
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"default":"$id.$key.transcript_fasta_uncompressed.fasta"
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}
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,
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"gene_bed_uncompressed": {
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"type":
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"string",
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"description": "Type: `file`, default: `$id.$key.gene_bed_uncompressed.bed`. ",
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"help_text": "Type: `file`, default: `$id.$key.gene_bed_uncompressed.bed`. "
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,
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"default":"$id.$key.gene_bed_uncompressed.bed"
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}
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,
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"star_index_uncompressed": {
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"type":
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"string",
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"description": "Type: `file`, default: `$id.$key.star_index_uncompressed.star_index_uncompressed`. Path to STAR index",
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"help_text": "Type: `file`, default: `$id.$key.star_index_uncompressed.star_index_uncompressed`. Path to STAR index."
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,
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"default":"$id.$key.star_index_uncompressed.star_index_uncompressed"
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}
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,
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"rsem_index_uncompressed": {
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"type":
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"string",
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"description": "Type: `file`, default: `$id.$key.rsem_index_uncompressed.rsem_index_uncompressed`. Path to directory or tar",
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"help_text": "Type: `file`, default: `$id.$key.rsem_index_uncompressed.rsem_index_uncompressed`. Path to directory or tar.gz archive for pre-built RSEM index."
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,
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"default":"$id.$key.rsem_index_uncompressed.rsem_index_uncompressed"
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}
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,
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"salmon_index_uncompressed": {
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"type":
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"string",
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"description": "Type: `file`, default: `$id.$key.salmon_index_uncompressed.salmon_index_uncompressed`. Path to Salmon index",
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"help_text": "Type: `file`, default: `$id.$key.salmon_index_uncompressed.salmon_index_uncompressed`. Path to Salmon index."
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,
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"default":"$id.$key.salmon_index_uncompressed.salmon_index_uncompressed"
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}
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,
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"kallisto_index_uncompressed": {
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"type":
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"string",
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"description": "Type: `file`, default: `$id.$key.kallisto_index_uncompressed.kallisto_index_uncompressed`. Path to Kallisto index",
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"help_text": "Type: `file`, default: `$id.$key.kallisto_index_uncompressed.kallisto_index_uncompressed`. Path to Kallisto index."
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,
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"default":"$id.$key.kallisto_index_uncompressed.kallisto_index_uncompressed"
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}
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,
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"bbsplit_index_uncompressed": {
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"type":
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"string",
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"description": "Type: `file`, default: `$id.$key.bbsplit_index_uncompressed.bbsplit_index_uncompressed`. Path to BBSplit index",
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"help_text": "Type: `file`, default: `$id.$key.bbsplit_index_uncompressed.bbsplit_index_uncompressed`. Path to BBSplit index."
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,
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"default":"$id.$key.bbsplit_index_uncompressed.bbsplit_index_uncompressed"
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}
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,
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"chrom_sizes": {
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"type":
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"string",
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"description": "Type: `file`, default: `$id.$key.chrom_sizes.sizes`. File containing chromosome lengths",
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"help_text": "Type: `file`, default: `$id.$key.chrom_sizes.sizes`. File containing chromosome lengths"
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,
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"default":"$id.$key.chrom_sizes.sizes"
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}
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,
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"fai": {
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"type":
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"string",
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"description": "Type: `file`, default: `$id.$key.fai.fai`. FASTA index file",
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"help_text": "Type: `file`, default: `$id.$key.fai.fai`. FASTA index file"
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,
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"default":"$id.$key.fai.fai"
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}
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}
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},
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"nextflow input-output arguments" : {
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"title": "Nextflow input-output arguments",
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"type": "object",
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"description": "Input/output parameters for Nextflow itself. Please note that both publishDir and publish_dir are supported but at least one has to be configured.",
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"properties": {
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"publish_dir": {
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"type":
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"string",
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"description": "Type: `string`, required, example: `output/`. Path to an output directory",
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"help_text": "Type: `string`, required, example: `output/`. Path to an output directory."
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}
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,
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"param_list": {
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"type":
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"string",
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"description": "Type: `string`, example: `my_params.yaml`. Allows inputting multiple parameter sets to initialise a Nextflow channel",
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"help_text": "Type: `string`, example: `my_params.yaml`. Allows inputting multiple parameter sets to initialise a Nextflow channel. A `param_list` can either be a list of maps, a csv file, a json file, a yaml file, or simply a yaml blob.\n\n* A list of maps (as-is) where the keys of each map corresponds to the arguments of the pipeline. Example: in a `nextflow.config` file: `param_list: [ [\u0027id\u0027: \u0027foo\u0027, \u0027input\u0027: \u0027foo.txt\u0027], [\u0027id\u0027: \u0027bar\u0027, \u0027input\u0027: \u0027bar.txt\u0027] ]`.\n* A csv file should have column names which correspond to the different arguments of this pipeline. Example: `--param_list data.csv` with columns `id,input`.\n* A json or a yaml file should be a list of maps, each of which has keys corresponding to the arguments of the pipeline. Example: `--param_list data.json` with contents `[ {\u0027id\u0027: \u0027foo\u0027, \u0027input\u0027: \u0027foo.txt\u0027}, {\u0027id\u0027: \u0027bar\u0027, \u0027input\u0027: \u0027bar.txt\u0027} ]`.\n* A yaml blob can also be passed directly as a string. Example: `--param_list \"[ {\u0027id\u0027: \u0027foo\u0027, \u0027input\u0027: \u0027foo.txt\u0027}, {\u0027id\u0027: \u0027bar\u0027, \u0027input\u0027: \u0027bar.txt\u0027} ]\"`.\n\nWhen passing a csv, json or yaml file, relative path names are relativized to the location of the parameter file. No relativation is performed when `param_list` is a list of maps (as-is) or a yaml blob.",
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"hidden": true
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}
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}
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}
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},
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"allOf": [
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{
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"$ref": "#/definitions/input"
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},
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{
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"$ref": "#/definitions/output"
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},
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{
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"$ref": "#/definitions/nextflow input-output arguments"
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}
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]
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}
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