2024-08-29 08:10:20 +00:00
|
|
|
name: htrnaseq
|
2025-05-07 10:40:18 +00:00
|
|
|
summary: |
|
|
|
|
|
A workflow for high-throughput RNA-seq data analyses.
|
2024-08-29 08:10:20 +00:00
|
|
|
description: |
|
2025-05-07 10:40:18 +00:00
|
|
|
This workflow is designed to process high-throughput RNA-seq data, where every
|
|
|
|
|
well of a microarray plate is a sample. A fasta file provided as input
|
|
|
|
|
defines the mapping between sample barcodes and wells.
|
|
|
|
|
|
|
|
|
|
The workflow is built in a modular fashion, where most of the base functionality
|
|
|
|
|
is provided by components from [`biobox`](https://www.viash-hub.com/packages/biobox/latest)
|
|
|
|
|
supplemented by custom base components and workflow components in this package.
|
|
|
|
|
|
|
|
|
|
The full workflow is split in two major subworkflows that can be run independently:
|
|
|
|
|
|
|
|
|
|
* **Well-demultiplexing:** Split the input (plate/pool level) fastq files per well.
|
|
|
|
|
* **Mapping, counting and QC:** Run per-well mapping, counting and generate QC reports.
|
|
|
|
|
|
|
|
|
|
Each of those can be started individually, or the full workflow can be run in two ways:
|
|
|
|
|
|
|
|
|
|
1. Run the [main workflow](https://www.viash-hub.com/packages/htrnaseq/v0.3.0/components/workflows/htrnaseq)
|
|
|
|
|
containing the main functionality.
|
|
|
|
|
2. Run the [(opinionated) `runner`](https://www.viash-hub.com/packages/htrnaseq/v0.3.0/components/workflows/runner) where a
|
|
|
|
|
number of choices (input/output structure and location) have been made.
|
|
|
|
|
|
|
|
|
|
Input for the workflow has to be `fastq` files (zipped or not). For bcl or other formats, please consider running
|
|
|
|
|
[demultiplex](https://www.viash-hub.com/packages/demultiplex) first.
|
|
|
|
|
|
2024-08-29 08:10:20 +00:00
|
|
|
license: MIT
|
2025-05-07 10:40:18 +00:00
|
|
|
keywords:
|
|
|
|
|
[
|
|
|
|
|
bioinformatics,
|
|
|
|
|
sequencing,
|
|
|
|
|
high-throughput,
|
|
|
|
|
RNAseq,
|
|
|
|
|
mapping,
|
|
|
|
|
counting,
|
|
|
|
|
pipeline,
|
|
|
|
|
workflow,
|
|
|
|
|
]
|
2024-08-29 08:10:20 +00:00
|
|
|
links:
|
|
|
|
|
issue_tracker: https://github.com/viash-hub/htrnaseq/issues
|
|
|
|
|
repository: https://github.com/viash-hub/htrnaseq
|
|
|
|
|
|
2025-04-29 13:35:02 +00:00
|
|
|
viash_version: 0.9.4
|
2024-11-05 17:26:35 +00:00
|
|
|
|
|
|
|
|
info:
|
|
|
|
|
test_resources:
|
2025-05-14 08:50:22 +00:00
|
|
|
- path: gs://viash-hub-resources/htrnaseq/v1
|
2024-11-05 17:26:35 +00:00
|
|
|
dest: resources_test
|
2024-08-29 08:10:20 +00:00
|
|
|
|
|
|
|
|
config_mods: |
|
|
|
|
|
.requirements.commands := ['ps']
|
|
|
|
|
.runners[.type == 'nextflow'].config.script := 'includeConfig("nextflow_labels.config")'
|
|
|
|
|
.resources += {path: '/src/config/labels.config', dest: 'nextflow_labels.config'}
|
2025-07-29 11:27:25 +00:00
|
|
|
.resources += {path: '/_viash.yaml', dest: '_viash.yaml'}
|
|
|
|
|
|